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Antibacterial Effects of Nisin and Chive Seed Extract on Staphylococcus aureus
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Zihao TIAN, Jinqi GUO, Xiangru WANG, Lili WEI, Tongjiao WANG, Ziyi ZHANG, Wei YU*, Jiage MA*
Science and Technology of Food Industry | 2026, 47(10) : 185 - 194
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Science and Technology of Food Industry | 2026, 47(10): 185-194
• Bioengineering •
Antibacterial Effects of Nisin and Chive Seed Extract on Staphylococcus aureus
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Zihao TIAN, Jinqi GUO, Xiangru WANG, Lili WEI, Tongjiao WANG, Ziyi ZHANG, Wei YU*, Jiage MA*
Affiliations
  • Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China
Published: 2026-05-15 doi: 10.13386/j.issn1002-0306.2025040375
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This study aimed to investigate the antibacterial effects of Nisin combined with chive seed extract (CSE) against Staphylococcus aureus and their effect on the quality attributes of strawberries and pasteurized milk during storage. The agar well diffusion assay was employed to assess the antibacterial activity of Nisin and CSE against Staphylococcus aureus ATCC 29213. Additionally, the antibacterial efficacy of Nisin and CSE against S. aureus was further investigated by measuring reactive oxygen species (ROS) levels and extracellular polymeric substance (EPS) content. Meanwhile, the effects of Nisin and CSE on the quality of the two food products were further investigated by determining the titratable acidity of pasteurized milk and strawberries during storage, as well as the weight loss and DPPH radical scavenging activity of strawberries and the protein content of pasteurized milk. The results demonstrated that the combination of Nisin and CSE exhibited synergistic antibacterial effect against S. aureus. The ROS levels of S. aureus were significantly reduced by 18.64% and 39.70% in Nisin+CSE group compared to Nisin and CSE groups, respectively (P<0.05). EPS content in S. aureus was remarkably decreased by 17.87% and 32.57% in Nisin+CSE group compared to Nisin and CSE groups, respectively (P<0.05). Furthermore, Nisin combined with CSE effectively delayed spoilage in both pasteurized milk and strawberries during 8-day storage at 25 ℃ and 4 ℃. The combination of Nisin and CSE exhibits a strong inhibitory effect against Staphylococcus aureus, offering a new strategy for the development of natural compound antibacterial agents.

Nisin  /  chive seed extract (CSE)  /  Staphylococcus aureus  /  synergistic antibacterial effect  /  pasteurized milk  /  strawberry
田子豪, 郭进琪, 王相茹, 魏丽郦, 王彤娇, 张子怡, 于微, 马佳歌. Nisin和韭菜籽提取物对金黄色葡萄球菌抑菌作用的研究. 食品工业科技, 2026 , 47 (10) : 185 -194 . DOI: 10.13386/j.issn1002-0306.2025040375
Zihao TIAN, Jinqi GUO, Xiangru WANG, Lili WEI, Tongjiao WANG, Ziyi ZHANG, Wei YU, Jiage MA. Antibacterial Effects of Nisin and Chive Seed Extract on Staphylococcus aureus[J]. Science and Technology of Food Industry, 2026 , 47 (10) : 185 -194 . DOI: 10.13386/j.issn1002-0306.2025040375
Year 2026 volume 47 Issue 10
140
64
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doi: 10.13386/j.issn1002-0306.2025040375
  • Receive Date:2025-04-30
  • Online Date:2026-10-24
  • Published:2026-05-15
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  • Received:2025-04-30
Affiliations
    Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China
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表12种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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