Full
Outline
To promote the development and application of Sibiraea angustata polysaccharides (SAPs), this study optimized the cellulase-ultrasonic synergistic extraction process of SAPs using response surface methodology (RSM), and further analyzed their structural characteristics and lipid-lowering activity. The results of process optimization indicated that the order of factors influencing the yield of SAPs was: solid-to-liquid ratio>enzyme addition amount >ultrasonic time. The optimal parameters for SAPs extraction were determined as follows: Liquid-to-solid ratio of 20 mL/g, enzyme addition of 3.5%, and ultrasonic time of 70 min, achieving a yield of 6.98% with extract rate of 65.23%. SAPs was an acidic heteropolysaccharide composed of 9 monosaccharide residues (Mw = 87.72 kDa), with highest galactose (32.9%), followed by rhamnose (25.7%), glucose (15%), and arabinose (12.6%). In addition, the IC50 values of SAPs for scavenging ability DPPH and ABTS+ radicals were 99.53 mg/L and 94.75 mg/L respectively, indicating their potential antioxidant activity. In terms of the lipid-lowering mechanism, SAPs exhibit a positive linear dependence on bile salt binding capacity. The binding rate of bile salt exceeds 60% at 5.0 mg/mL SAPs, with the strongest binding ability observed with taurocholate. With increasing concentrations of SAPs, the levels of triglycerides (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) in zebrafish exhibited a dose-dependent decrease. Oil Red O staining further confirmed that SAPs significantly reduced lipid droplet deposition in the zebrafish tail, with the effect demonstrating a concentration-dependent manner. The lipid-lowering effect of SAPs was significantly concentration-dependent (P<0.05). The lipid-lowering rate reached 9.85% at 5.0 mg/mL SAPs, demonstrating that SAPs could effectively ameliorate hyperlipidemia through dose regulation. These results will lay a theoretical and technical foundation for the development of functional lipid-lowering products using SAPs.
105
41
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
