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To optimize the extraction of Ganoderma lucidum mycelium polysaccharides (GLP) derived from the mycelium of Ganoderma lucidum CZDG10 and to explore its antioxidant properties and protective effect on skin cell damage. The extraction of GLP was optimized using a combination of single factor experiments and the response surface method, with high-pressure crushing assistance and compound enzyme treatment. The antioxidant activity of GLP was analyzed, and the injury model of human skin fibroblasts (HSF) induced by H2O2 was evaluated to assess the efficacy of GLP in preventing cell damage. The optimal extraction conditions of GLP included a crushing pressure of 100 MPa, solid-liquid ratio of 1:40 g/mL, compound enzyme content of 2.40%, enzymatic temperature of 50.0 ℃, enzymatic pH of 5.5, and enzymatic reaction time of 2.25 h. Under these conditions, GLP was obtained in 9.69%±0.32% yield. GLP primarily comprised mannose, glucose, galactose, and fucose and effectively scavenged DPPH, OH, and ABTS＋ free radicals, with IC50 values of 0.30, 0.36, and 1.59 mg/mL, respectively, demonstrating its antioxidant capacity. Models of H2O2-induced HSF oxidative stress showed that GLP could improve the cell survival rate in a concentration-dependent manner and increase cell viability by 22.33% at a concentration of 1.0 mg/mL. GLP also up-regulated the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in HSF cells under oxidative stress induced by H2O2 and reduced the content of reactive oxygen species (ROS) and malondialdehyde (MDA), thereby reducing oxidative stress damage. In addition, GLP increased the content of collagen in HSF cells by reducing the matrix metalloproteinase-1 (MMP-1) content and promoting the secretion of collagen I in HSF cells, thereby maintaining the healthy state of the skin cells. Therefore, GLP exhibited a significant protective effect against H2O2-induced HSF cell damage. This study provides a theoretical basis for the development and application of polysaccharides from Ganoderma lucidum mycelium.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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