Home Journals Articles Updates About
CN
image
收藏切换
Investigating the Digestive and Absorptive Mechanisms of Polysaccharides from Huaishan Based on Intestinal Organoids and Their Effects on Intestinal Cell Proliferation and Differentiation
收藏切换
Mengshi SONG1, Xiaoling ZENG2, Jing LI2, Jiaqi WANG2, Wei LI2, Yi XIAO1, Chuxin LIU1, Qiuyi LI1, Chaoyue WEN1, Qingwu SHEN1, Yanyang WU1, 3, 4, *
Science and Technology of Food Industry | 2026, 47(10) : 438 - 446
Less
收藏切换
Science and Technology of Food Industry | 2026, 47(10): 438-446
• Nutrition and Healthcare •
Investigating the Digestive and Absorptive Mechanisms of Polysaccharides from Huaishan Based on Intestinal Organoids and Their Effects on Intestinal Cell Proliferation and Differentiation
Full
Mengshi SONG1, Xiaoling ZENG2, Jing LI2, Jiaqi WANG2, Wei LI2, Yi XIAO1, Chuxin LIU1, Qiuyi LI1, Chaoyue WEN1, Qingwu SHEN1, Yanyang WU1, 3, 4, *
Affiliations
  • 1Key Laboratory for Food Science and Biotechnology of Hunan Province, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
  • 2Ausnutria Dairy (China) Co., Ltd., Changsha 410000, China
  • 3Horticulture and Landscape College, Hunan Agricultural University, Changsha 410128, China
  • 4State Key Laboratory of Subhealth Intervention Technology, Changsha 410128, China
Published: 2026-05-15 doi: 10.13386/j.issn1002-0306.2025050111
Outline
收藏切换

To contribute to better understanding of the structural composition, bioactivity, and intestinal health effects of Huaishan polysaccharides (HSP), analyzed the changes in the molecular weight and monosaccharide composition of HSP after digestion with simulated gastric juice, and evaluated its impact on intestinal cell proliferation and differentiation using a three-dimensional (3D) organoid model. The study findings showed that Huaishan polysaccharide molecular weights averaged 139.173 kDa prior to source plant digestion, and 1.676 kDa after digestion. Prior to digestion, D-glucose (89.72%) was the dominant monosaccharide, but its levels decline significantly after digestion, while levels of other monosaccharides (e.g., D-mannose, D-galactose) tend to rise. Immunofluorescence analyses confirmed key intestinal cell markers, including Ki67 (proliferation), Chromogranin A (secretory), Mucin2 (goblet cells), Lysozyme (Paneth cells), Villim (absorptive), and Olfm4 (ISCs), were present in the 3D organoids. Chinese yam polysaccharide-treated organoids exhibited significantly enhanced mRNA levels and immunofluorescence signals for Ki67 and Muc2. Huangshan polysaccharides also upregulated respective Lgr5, Olfm4, Wnt3, and Hes1 mRNA levels 1.32, 1.15, 1.1, and 1.12 fold (P<0.05). These data suggested that Huaishan polysaccharides enhance ISC proliferation via Wnt-mediated Lgr5/Olfm4 activation while promoting goblet cell differentiation via Notch inhibition. These data revealed the structure of Huaishan polysaccharides at stages during digestion, and its intestinal proliferation and differentiation promoting effects, laying a foundation for Huaishan polysaccharide application in functional foods.

Huaishan polysaccharides  /  intestinal organoids  /  gastrointestinal simulation digestion  /  monosaccharide composition  /  intestinal stem cells  /  Lgr5  /  Wnt3
宋梦诗, 曾小玲, 李净, 汪家琦, 李威, 肖毅, 刘楚鑫, 李秋怡, 文超越, 沈清武, 吴艳阳. 基于肠类器官探究淮山多糖的消化吸收机制及其对肠细胞增殖分化的影响. 食品工业科技, 2026 , 47 (10) : 438 -446 . DOI: 10.13386/j.issn1002-0306.2025050111
Mengshi SONG, Xiaoling ZENG, Jing LI, Jiaqi WANG, Wei LI, Yi XIAO, Chuxin LIU, Qiuyi LI, Chaoyue WEN, Qingwu SHEN, Yanyang WU. Investigating the Digestive and Absorptive Mechanisms of Polysaccharides from Huaishan Based on Intestinal Organoids and Their Effects on Intestinal Cell Proliferation and Differentiation[J]. Science and Technology of Food Industry, 2026 , 47 (10) : 438 -446 . DOI: 10.13386/j.issn1002-0306.2025050111
Appendix
Less
Year 2026 volume 47 Issue 10
114
44
Cite this Article
BibTeX
Article Info
doi: 10.13386/j.issn1002-0306.2025050111
  • Receive Date：2025-05-14
  • Online Date：2026-10-24
  • Published：2026-05-15
Article Data
Affiliations
History
  • Received：2025-05-14
Affiliations
    1Key Laboratory for Food Science and Biotechnology of Hunan Province, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
    2Ausnutria Dairy (China) Co., Ltd., Changsha 410000, China
    3Horticulture and Landscape College, Hunan Agricultural University, Changsha 410128, China
    4State Key Laboratory of Subhealth Intervention Technology, Changsha 410128, China
References
Share
https://castjournals.cast.org.cn/joweb/spgykj/EN/10.13386/j.issn1002-0306.2025050111
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House