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Preparation of Zearalenone Metal Phenolic Network-coated Immunomagnetic Beads and Their Application in Pre-treatment of the Assay
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Jiawen CHEN1, 2, Jiaxin CHEN1, 2, Yingjie DU3, Shan JIAN2, 4, Lingbo ZHAO2, 4, Baoqing ZHOU2, 3, Wei ZHANG2, 4, Yonghua XIONG3, Ping GUO2, 4, *
Science and Technology of Food Industry | 2026, 47(1) : 348 - 356
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Science and Technology of Food Industry | 2026, 47(1): 348-356
• Analysis and Determination •
Preparation of Zearalenone Metal Phenolic Network-coated Immunomagnetic Beads and Their Application in Pre-treatment of the Assay
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Jiawen CHEN1, 2, Jiaxin CHEN1, 2, Yingjie DU3, Shan JIAN2, 4, Lingbo ZHAO2, 4, Baoqing ZHOU2, 3, Wei ZHANG2, 4, Yonghua XIONG3, Ping GUO2, 4, *
Affiliations
  • 1College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330000, China
  • 2Jiangxi Provincial General Institute of Testing and Certification Food Inspection and Testing Research Institute, Nanchang 330200, China
  • 3College Food Science & Technology, Nanchang University, Nanchang 330000, China
  • 4Nanchang Key Laboratory of Food Rapid Testing, Nanchang 330200, China
Published: 2026-01-01 doi: 10.13386/j.issn1002-0306.2025010234
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The present study aimed to establish a pretreatment method for specific and rapid enrichment of zearalenone (ZEN) using metal-phenolic network (MPN)-modified immunomagnetic beads (IMBs) for efficient detection of ZEN in grains and feed. Surfaces of Fe3O4@SiO2-NH2 magnetic microspheres were coated with MPNs to enable directional immobilization of anti-ZEN antibodies via metal-phenolic hydroxyl coordination and hydrogen bonding. The results revealed that at pH5.5, 80 μg antibody immobilized per mg magnetic beads, and 10 min incubation duration, the IMBs had a saturated adsorption capacity of 35 ng/mg, with the Langmuir adsorption equilibrium constant KL of 595.1 L/mg. Spiked recovery tests performed in corn, wheat, and feed matrices, with recoveries >90% and coefficients of variation (CVs) within 1.3%~4.5% at spiking levels of 30~60 µg/kg. At the elevated spiking level of 120 µg/kg, consistent recoveries of 81.0%, 83.7%, and 77.6% were maintained with CVs≤6.5% across the three matrices. This method demonstrated excellent anti-interference performance towards interfering substances such as starch and pigments in complex substrates, including corn, wheat, and feed. When this method and the immunoaffinity column method were separately applied for sample pretreatment, the results showed a relative standard deviation of ≤7.30%, indicating good consistency between the two methods. By achieving synergy between specific recognition and controllable dissociation, our strategy provides a reliable solution for efficient ZEN enrichment in grains and feed.

zearalenone  /  immunomagnetic beads  /  magnetic enrichment  /  cereal  /  feed
陈佳文, 陈嘉欣, 杜英杰, 简姗, 赵令波, 周宝青, 张威, 熊勇华, 郭平. 玉米赤霉烯酮金属酚醛网络涂层免疫磁珠的制备及在检测前处理中的应用. 食品工业科技, 2026 , 47 (1) : 348 -356 . DOI: 10.13386/j.issn1002-0306.2025010234
Jiawen CHEN, Jiaxin CHEN, Yingjie DU, Shan JIAN, Lingbo ZHAO, Baoqing ZHOU, Wei ZHANG, Yonghua XIONG, Ping GUO. Preparation of Zearalenone Metal Phenolic Network-coated Immunomagnetic Beads and Their Application in Pre-treatment of the Assay[J]. Science and Technology of Food Industry, 2026 , 47 (1) : 348 -356 . DOI: 10.13386/j.issn1002-0306.2025010234
Appendix
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Year 2026 volume 47 Issue 1
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doi: 10.13386/j.issn1002-0306.2025010234
  • Receive Date：2025-01-23
  • Online Date：2026-10-24
  • Published：2026-01-01
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  • Received：2025-01-23
Affiliations
    1College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330000, China
    2Jiangxi Provincial General Institute of Testing and Certification Food Inspection and Testing Research Institute, Nanchang 330200, China
    3College Food Science & Technology, Nanchang University, Nanchang 330000, China
    4Nanchang Key Laboratory of Food Rapid Testing, Nanchang 330200, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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