Home Journals Articles Updates About
CN
image
收藏切换
Enzymatic Preparation and Characterization of DPP-IV Inhibitory Peptides Derived from Corn Protein
收藏切换
Li TAO1, Juntong WANG1, 2, Guanlong LI3, Caixia JIANG4, Xiqun ZHENG1, 2, 3, *, Xiaolan LIU3, *
Science and Technology of Food Industry | 2026, 47(1) : 289 - 299
Less
收藏切换
Science and Technology of Food Industry | 2026, 47(1): 289-299
• Processing Technology •
Enzymatic Preparation and Characterization of DPP-IV Inhibitory Peptides Derived from Corn Protein
Full
Li TAO1, Juntong WANG1, 2, Guanlong LI3, Caixia JIANG4, Xiqun ZHENG1, 2, 3, *, Xiaolan LIU3, *
Affiliations
  • 1College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China
  • 2Engineering Research Center of Processing and Utilization of Grain By-products and Utilizationof Ministry of Education, Daqing 163319, China
  • 3College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, China
  • 4National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Published: 2026-01-01 doi: 10.13386/j.issn1002-0306.2025010123
Outline
收藏切换

In this work, bioactive peptides with inhibitory effect on dipeptidyl peptidase-IV (DPP-IV) were prepared from corn protein meal. The protease with the best inhibitory effect on DPP-IV was selected by enzymatic preparation technique, and the optimal enzymatic hydrolysis conditions were determined by single factor and response surface optimization experiments. The molecular weight distribution, ultrafiltration classification and amino acid composition of the obtained inhibitory peptides were further analyzed. The results showed that the neutral protease had the best effect on the preparation of DPP-IV inhibitory peptide from corn protein source. The optimal enzymolysis conditions were substrate concentration of 10% (w/v), enzyme dosage of 640 U/g, enzymolysis time of 157 min, enzymolysis temperature of 45 ℃ and enzymolysis pH of 7.0. The half-inhibitory concentration (IC50) value of DPP-IV for the inhibitory peptide obtained under this condition was 0.53±0.06 mg/mL. After ultrafiltration, the molecular weight of components less than 1 kDa accounted for 58.9% of the original inhibitory peptide, and the inhibitory activity against DPP-IV was higher than that of the original inhibitory peptide, which was related to its amino acid composition. The results of enzymatic reaction kinetics showed that the inhibition type of DPP-IV was competitive and reversible. The results of this study provide a theoretical basis for the development and utilization of DPP-IV inhibitory peptide from corn protein source, and suggest a new idea for the high-value utilization of corn protein.

corn-protein-derived inhibitory peptides  /  neutral protease  /  dipeptidyl peptidase-IV (DPP-IV)  /  type 2 diabetes (T2DM)
陶莉, 王俊彤, 李冠龙, 姜彩霞, 郑喜群, 刘晓兰. 玉米蛋白源DPP-IV抑制肽的酶法制备及其性质研究. 食品工业科技, 2026 , 47 (1) : 289 -299 . DOI: 10.13386/j.issn1002-0306.2025010123
Li TAO, Juntong WANG, Guanlong LI, Caixia JIANG, Xiqun ZHENG, Xiaolan LIU. Enzymatic Preparation and Characterization of DPP-IV Inhibitory Peptides Derived from Corn Protein[J]. Science and Technology of Food Industry, 2026 , 47 (1) : 289 -299 . DOI: 10.13386/j.issn1002-0306.2025010123
Appendix
Less
Year 2026 volume 47 Issue 1
110
45
Cite this Article
BibTeX
Article Info
doi: 10.13386/j.issn1002-0306.2025010123
  • Receive Date：2025-01-14
  • Online Date：2026-10-24
  • Published：2026-01-01
Article Data
Affiliations
History
  • Received：2025-01-14
Affiliations
    1College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China
    2Engineering Research Center of Processing and Utilization of Grain By-products and Utilizationof Ministry of Education, Daqing 163319, China
    3College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, China
    4National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China
References
Share
https://castjournals.cast.org.cn/joweb/spgykj/EN/10.13386/j.issn1002-0306.2025010123
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House