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Optimization of the Rice Wine Fermentation Process for Beef Jerky and Changes in Myofibrillar Proteins
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Jia MIAO, Ziyuan ZHANG, Wenhui LIU, Baohua KONG, Qian LIU, Fangda SUN*
Science and Technology of Food Industry | 2026, 47(1) : 250 - 261
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Science and Technology of Food Industry | 2026, 47(1): 250-261
• Processing Technology •
Optimization of the Rice Wine Fermentation Process for Beef Jerky and Changes in Myofibrillar Proteins
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Jia MIAO, Ziyuan ZHANG, Wenhui LIU, Baohua KONG, Qian LIU, Fangda SUN*
Affiliations
  • College of Food Science, Northeast Agricultural University, Harbin 150030, China
Published: 2026-01-01 doi: 10.13386/j.issn1002-0306.2024110303
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The objective of this study was to investigate the effect of rice wine on the quality of beef jerky. The beef jerky fermentation process was optimized by determining the moisture content, color difference, pH, shear force, texture, and water activity, with sensory evaluation scores serving as the response variables in a response surface methodology experiment. Additionally, changes in myofibrillar proteins during fermentation were thoroughly examined using solubility measurement, surface hydrophobicity assessment, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The optimal processing conditions were rice wine addition 6%, fermentation time 24 h, and baking time 2.5 h. The beef jerky produced under these conditions exhibited physicochemical properties with moisture content 42.39%, pH 5.61, water activity 0.9298 and shear force 28.97 N. The fermented beef jerky prepared using this process had excellent color, moderate texture, unique flavor, and high overall quality. During the fermentation process, the solubility of myofibrillar proteins in beef gradually decreased with increasing fermentation time, whereas its surface hydrophobicity continuously increased. This phenomenon indicated that the fermentation promoted the gradual unfolding of the highly aggregated structure of myofibrillar proteins, resulting in the exposure of internal hydrophobic groups. The SDS-PAGE results demonstrated that the addition of rice wine was more conducive to the degradation of myofibrillar proteins. The findings of this study provide important theoretical support for the development of new fermented beef jerky products, and offer new insights for research in related fields.

fermented beef jerky  /  rice wine  /  sensory quality  /  response surface optimization  /  myofibrillar protein
苗嘉, 张紫媛, 刘文惠, 孔保华, 刘骞, 孙方达. 醪糟发酵牛肉干工艺优化及肌原纤维蛋白变化研究. 食品工业科技, 2026 , 47 (1) : 250 -261 . DOI: 10.13386/j.issn1002-0306.2024110303
Jia MIAO, Ziyuan ZHANG, Wenhui LIU, Baohua KONG, Qian LIU, Fangda SUN. Optimization of the Rice Wine Fermentation Process for Beef Jerky and Changes in Myofibrillar Proteins[J]. Science and Technology of Food Industry, 2026 , 47 (1) : 250 -261 . DOI: 10.13386/j.issn1002-0306.2024110303
Year 2026 volume 47 Issue 1
130
58
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doi: 10.13386/j.issn1002-0306.2024110303
  • Receive Date:2024-11-25
  • Online Date:2026-10-24
  • Published:2026-01-01
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  • Received:2024-11-25
Affiliations
    College of Food Science, Northeast Agricultural University, Harbin 150030, China
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表12种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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