Full
Outline
To enhance the solubility of rice bran protein (RBP) and thereby improve its application performance in food processing, the pH-driven method was employed in this study, under which RBP was treated at pH conditions of 1.5, 2.5, 3.5, 10, 11, and 12, and structural and functional properties before and after modification were compared and analyzed. The results showed that the solubility of RBP was observed to be gradually increased with rising pH, with the optimal effect achieved at pH12, where a solubility of 70.57% was reached, representing a significant improvement of 63.85% compared to the untreated group (6.72%). The average particle size, absolute ζ-potential value, and surface hydrophobicity of RBP were found to be significantly altered as the pH increased; After treatment at pH12, the average particle size was reduced to the minimum (140.47 nm), the absolute ζ-potential value was increased to the maximum (14.8 mV), and the surface hydrophobicity was elevated to the highest value (2289), along with a marked enhancement in intrinsic fluorescence intensity and a red shift in the maximum emission wavelength. Following pH12 treatment, the relative content of β-sheets in RBP was increased by 10.43%, while the relative contents of α-helices and β-turns were reduced by 3.29% and 9.13%, respectively. These findings indicate that pH-driven method can induce depolymerization of RBP molecules and strengthen intermolecular electrostatic repulsion, thereby significantly improving its solubility. This study offers a new approach to promote the widespread application of RBP.
101
48
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
