Full
Outline
Objective: This study aimed to investigate the efficacy and mechanisms by which Chuju flavonoids ameliorated lipid metabolism disorders induced by a high-fat diet in mice, thereby providing a theoretical foundation for dietary interventions in nonalcoholic fatty liver disease (NAFLD). Methods: Seventy male C57BL/6 mice were acclimatized for one week and subsequently assigned to the following groups: Normal diet (ND), high-fat diet with low-dose Chuju flavonoid-rich extracts (CJEL), positive control (CJE), model group (HFD), and high-fat diet with high-dose CJE (CJEH). Modeling and administration occurred concurrently over a period of 14 weeks. Measurements included body weight, organ indices, and serum and hepatic lipid parameters. The effects of CJE on lipid metabolism disorders induced by a high-fat diet were evaluated through histopathological staining, gut microbiota 16S rRNA sequencing, and Western blot analysis. Results: High-dose Chuju flavonoids significantly decreased total cholesterol (39%±3.8%), triglycerides (41.85%±3.75%), and non-esterified free fatty acids (31.1%±2.4%) in the livers of mice subjected to a high-fat diet. Additionally, these flavonoids inhibited the activity of alanine aminotransferase (32.4%) and aspartate aminotransferase (29.8%). Histopathological analysis demonstrated that CJE markedly reduced hepatic lipid accumulation, as evidenced by a 62.5%±6.2% decrease in lipid droplet area in NAFLD mice. Furthermore, 16S rRNA sequencing confirmed that CJE altered the gut microbiota and activated the AMPK/PPAR-α signaling pathway, which inhibited lipid synthesis and enhanced fatty acid β-oxidation. Conclusion: CJE significantly ameliorates lipid metabolism abnormalities in NAFLD mice by restoring gut microbiota balance, activating the AMPK/PPAR-α pathway to suppress hepatic lipid synthesis, and promoting hepatic fatty acid oxidation. This approach offers a viable dietary intervention strategy for the prevention of NAFLD.
121
52
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
