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Outline
White-leg shrimp (Litopenaeus vannamei) is prone to spoilage and deterioration during storage due to microbial contamination and the action of endogenous enzymes. This study focused on white-leg shrimp, Stenotrophomonas acidaminiphila (S-1), and myofibrillar protein, the key quality component of shrimp, with the aim of multidimensionally exploring the inhibitory effect of the metabolites of Paenibacillus provencensis (PP-2) on S-1 and the effect on myofibrillar protein properties. By using techniques such as non-targeted metabolomics, it aimed to explore the preservative effects of the metabolites of Paenibacillus provencensis (PP-2) on white-leg shrimp from multiple dimensions. Using non-targeted metabolomics and other techniques, it was found that there were 20 antibacterial active substances in the metabolites of PP-2. When these metabolites acted on S-1, they could change the cell membrane permeability, resulting in the leakage of intracellular proteins and ultimately the death of bacteria, thus achieving antibacterial effects. Compared with the metabolites of Nisin, Kazachstania servazzii (3-D), Saccharomyces cerevisiae (7-C), and Lactobacillus plantarum, at the end of storage, the protein concentration in the group treated with PP-2 metabolites was relatively high, the sulfhydryl group content and Ca2+-ATPase activity were relatively high, and it had the best effect in maintaining the secondary structure of proteins. This study clarifies the antibacterial mechanism of PP-2 metabolites and their effects on the protein characteristics of white-leg shrimp, opening up a new path for the biological preservation technology of white-leg shrimp and providing a theoretical basis for the application of PP-2 metabolites in the field of aquatic product preservation.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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