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Screening, Identification, Whole Genome Analysis of Four Agarase-producing Vibrio Strains and Their Enzymatic Properties
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Liqun HUANG1, 2, 3, Lianping ZHANG1, 2, 3, Shuyi FAN1, 2, 3, Liling YAO1, 2, 3, Chentao YAN1, 2, 3, Xulei ZHENG1, 2, 3, Bin LIU1, 2, 3, 4, *, Ying HUANG1, 2, 3, 4, *
Science and Technology of Food Industry | 2026, 47(11) : 213 - 225
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Science and Technology of Food Industry | 2026, 47(11): 213-225
• Bioengineering •
Screening, Identification, Whole Genome Analysis of Four Agarase-producing Vibrio Strains and Their Enzymatic Properties
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Liqun HUANG1, 2, 3, Lianping ZHANG1, 2, 3, Shuyi FAN1, 2, 3, Liling YAO1, 2, 3, Chentao YAN1, 2, 3, Xulei ZHENG1, 2, 3, Bin LIU1, 2, 3, 4, *, Ying HUANG1, 2, 3, 4, *
Affiliations
  • 1College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • 2Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou 350002, China
  • 3Fujian Agricultural Products Preservation Technology Development Base, Fuzhou 350002, China
  • 4Fuzhou Institute of Oceanography, Fuzhou 350108, China
Published: 2026-06-01 doi: 10.13386/j.issn1002-0306.2025060176
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In order to expand the reservoir of highly active agarase-producing strains and to mine agarase-related gene pools, this study isolated strains from abalone viscera. Primary screening was conducted using a single carbon source medium combined with Lugo's iodine staining, followed by rescreening via the 3,5-dinitrosalicylic acid (DNS) method to determine enzyme activity. Agarase-producing strains were identified by 16S rDNA sequencing, and their genomic sequences and enzymatic properties were compared. Four strains of Vibrio mediterranei (2DB7, 2DB15, 2DBB20 and 2DBB51) producing highly active agarases were screened. The activities of the crude enzyme solutions from these strains were 315.17, 340.99, 293.88, and 324.37 U/mL, respectively. Whole genome sequencing revealed that 2DB15, 2DBB20, and 2DBB51 encode relatively abundant agarase genes and related enzyme genes, including β-agarases (GH16 and GH50 families), β-galactosidase, and α-neoagarobiose hydrolase. The agarases produced by these four strains (2DB7, 2DB15, 2DBB20 and 2DBB51) reached their peak relative activity at 45, 40, 45, and 45 ℃, and at pH 8.0, 7.0, 8.0, and 7.0, respectively. Furthermore, all enzymes maintained good stability below 40 ℃ and within a pH range of 5.0 to 9.0. The research results could enrich the theoretical foundation for agarase production by Vibrio sp. and provide high-quality enzyme resources for the eco-friendly production of agar oligosaccharides.

agarase  /  Vibrio mediterranei  /  whole genome sequencing  /  enzymatic properties
黄立群, 张联平, 范舒怡, 姚丽玲, 燕辰涛, 郑旭蕾, 刘斌, 黄莹. 四株产琼胶酶弧菌的筛选鉴定、全基因组分析及其产酶酶学性质研究. 食品工业科技, 2026 , 47 (11) : 213 -225 . DOI: 10.13386/j.issn1002-0306.2025060176
Liqun HUANG, Lianping ZHANG, Shuyi FAN, Liling YAO, Chentao YAN, Xulei ZHENG, Bin LIU, Ying HUANG. Screening, Identification, Whole Genome Analysis of Four Agarase-producing Vibrio Strains and Their Enzymatic Properties[J]. Science and Technology of Food Industry, 2026 , 47 (11) : 213 -225 . DOI: 10.13386/j.issn1002-0306.2025060176
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Year 2026 volume 47 Issue 11
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doi: 10.13386/j.issn1002-0306.2025060176
  • Receive Date：2025-06-16
  • Online Date：2026-10-22
  • Published：2026-06-01
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  • Received：2025-06-16
Affiliations
    1College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
    2Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou 350002, China
    3Fujian Agricultural Products Preservation Technology Development Base, Fuzhou 350002, China
    4Fuzhou Institute of Oceanography, Fuzhou 350108, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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