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This study employed response surface methodology to optimize the preparation process of phosphorylated chicory polysaccharide iron. It characterized the structure of the composite using Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM). Through simulated artificial gastrointestinal fluid digestion experiments, sucrase hydrolysis experiments, and mucin-binding capacity assays, the in vitro digestive characteristics of phosphorylated chicory polysaccharide iron were systematically analyzed. The results indicated that the optimal reaction conditions were a reaction time of 1.1 h, a reaction temperature of 64 ℃, and a pH of 10. Under these conditions, the iron content of the prepared phosphorylated chicory polysaccharide iron reached 26.97%±0.25%. Infrared spectroscopy of phosphorized chicory polysaccharide iron showed that the O-H and C=O groups in the phosphorized chicory polysaccharide coordinate with Fe3+. XPS confirmed that iron was introduced into the phosphorized polysaccharide in the trivalent state (Fe3+). SEM observation revealed that the composite morphology was a dense layered structure. In vitro gastrointestinal digestion results showed that the iron retention rate in gastric fluid was >97% after 24 hours, while in the simulated intestinal environment containing mucin and sucrase, 59% was released after 7 hours, with a cumulative release rate of 74.5%±0.5% after 24 hours. This study provides a theoretical basis for the high-value utilization of chicory polysaccharide resources and the development of polysaccharide iron supplements.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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