Full
Outline
Objective: We optimized the sulfation modification process of Moutan cortex polysaccharide (MCP) and systematically evaluated the structural characteristics, antioxidant activity, and hypoglycemic potency of sulfated MCP (S-MCP). Methods: MCP were sulfated using concentrated sulfuric acid method. The process parameters were optimized using single-factor experiments, response surface methodology, and using degree of substitution (DS) as an index. S-MCP structure was characterized by FTIR, DLS, and SEM. Its antioxidant and hypoglycemic activities were evaluated in vitro and in vivo. Results: Optimal sulfation conditions for MCP included 39 min esterification time, 1.5 mL concentrated sulfuric acid, and 30 mg of ammonium sulfate. Under these conditions, the DS of S-MCP was 0.07391±0.0020. Structural characterization confirmed successful introduction of sulfate groups onto S-MCP. The S-MCP particle size (294.44±40.62 nm) was smaller than that of MCP (356.32±26.07 nm), and its microscopic characteristics changed from loose and porous to fragmented and layered. In vitro activity experiments showed respective scavenging rates of S-MCP against DPPH, ABTS+, and hydroxyl radicals of 64.85%, 82.42%, and 71.34% at 3.0 mg/mL significantly higher than those of MCP. Scavenging was also shown to be concentration-dependent. S-MCP displayed more potent inhibition of α-glucosidase (43.92% at 1.0 mg/mL) than MCP (31.31%). The mouse glucose tolerance test results showed that S-MCP significantly reduced the blood glucose levels after starch and sucrose loading, and decreased the area under the curve. Conclusion: Sulfation can effectively enhance MCP antioxidant and hypoglycemic activities, providing a scientific basis for their development and application in functional foods and pharmaceuticals.
123
61
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
