Full
Outline
To investigate the effects of different Ca2+ concentrations (control group, Ca1 0.86 mmol/L, Ca2 2.58 mmol/L, Ca3 17.2 mmol/L, Ca4 34.4 mmol/L) on the structure and physicochemical properties of catechin-ovalbumin amyloid fibril hydrogels (C-OVAF), conducted a systematic analysis using techniques such as thioflavin T fluorescence spectroscopy, circular dichroism spectroscopy, rheology, low-field NMR, particle size analysis, Zeta potential measurement, surface hydrophobicity assessment, antioxidant experiments, scanning electron microscopy, and molecular docking. The results showed that, compared with the control group, Ca2+ cross-linking significantly altered the secondary structure (α-helix increased from 6% in the control to 12% in Ca4, β-turns increased from 16% (control) to 29% (Ca4), β-sheets decreased from 50% (control) to 43% (Ca4), and random coils decreased from 28% (control) to 15% (Ca3). As Ca2+ concentration increased, the storage modulus (G') first rose and then fell, with the Ca2+ group exhibiting the highest G' (indicating the highest network density and elasticity). Low-field nuclear magnetic resonance confirmed an increase in the proportion at this concentration, with significantly optimized water retention, the lowest surface hydrophobicity, and DPPH, ABTS+ radicals reached 61.9% and 53.4%, respectively, with optimal antioxidant activity, while the system's particle size and Zeta potential stabilized. Scanning electron microscopy revealed that the Ca2 group formed a regular, continuous network structure, and molecular docking confirmed that C-OVAF forms complexes via hydrophobic interactions and hydrogen bonding, with Ca2+ in the Ca2 group enhancing intermolecular interactions through coordination. In summary, Ca2+ crosslinking significantly improves the structure and properties of C-OVAF hydrogels. A Ca2+ concentration of 2.58 mmol/L balances molecular intermolecular forces to simultaneously optimize water retention and antioxidant activity. This study provides theoretical guidance for the performance regulation, preparation optimization, and food processing applications of metal ion-crosslinked polyphenol-protein hydrogels.
122
46
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
