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Effect of Additional Amino Acids on the Cysteine-Xylose Maillard Reaction to Form Volatile Flavor Compounds
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Jinxin HE, Kexin LI, Yu LIANG, Qingqing HU, Jianchun XIE*
Science and Technology of Food Industry | 2026, 47(13) : 262 - 271
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Science and Technology of Food Industry | 2026, 47(13): 262-271
• Analysis and Determination •
Effect of Additional Amino Acids on the Cysteine-Xylose Maillard Reaction to Form Volatile Flavor Compounds
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Jinxin HE, Kexin LI, Yu LIANG, Qingqing HU, Jianchun XIE*
Affiliations
  • School of Food and Health, Beijing Technology and Business University (BTBU), Key Laboratory of Geriatric Nutrition and Health, Ministry of Education, Beijing 100048, China
Published: 2026-07-01 doi: 10.13386/j.issn1002-0306.2025040257
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This study investigated effect of adding serine, glutamic acid, or glycine to the "cysteine-xylose" system on formation of volatile flavor compounds via the Maillard reaction, aiming to provide guidance for meat animals breeding, meat food processing, and meat flavorings preparation. For the different Maillard reaction systems, their reaction extents were assessed by measurement of UV absorption at 294 and 420 nm, color difference values, pH, and the remaining xylose in the reaction solutions. Volatile flavor compounds generated were comparatively analyzed by solid-phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) and aroma extract dilution analysis in gas chromatography-olfactometry (AEDA/GC-O) in combination with partial least squares regression (PLSR) analysis and odor activity value (OAV) calculation. The results showed that addition of each of the three amino acids in four times the molar concentration of cysteine, the reaction extents of the Maillard reaction systems were enhanced. Addition of glycine caused the most browning of the reaction solution, followed by that of serine and glutamic acid. On the other hand, formation of volatile flavor compounds also increased as well with addition of the three amino acids. Especially, addition of glutamic acid produced the highest amount of sulfur-containing meaty compounds, where the odor-active compounds of them had greater dilution factors and the key aroma compounds (OAV≥1) of them exhibited larger OAVs. As the three amino acids were increasingly added to "cysteine-xylose" system, amounts of sulfur-containing key aroma compounds generated in the reaction systems first rose then fell, whereas amounts of the pyrazine key aroma compounds ascended all the way. As serine and glutamic acid each were in four times cysteine concentration, and glycine was in two times cysteine concentration, the sulfur-containing key aroma compounds were maximally yielded in the reaction systems, respectively. In conclusion, among the three amino acids, glutamic acid caused the weakest browning reaction and benefitted generation of sulfur-containing meaty compounds.

cysteine  /  glycine  /  serine  /  glutamic acid  /  meat flavor  /  Maillard reaction
何金鑫, 李珂欣, 梁雨, 胡晴晴, 谢建春. 添加氨基酸对“半胱氨酸-木糖”美拉德反应体系产生挥发性风味化合物的影响. 食品工业科技, 2026 , 47 (13) : 262 -271 . DOI: 10.13386/j.issn1002-0306.2025040257
Jinxin HE, Kexin LI, Yu LIANG, Qingqing HU, Jianchun XIE. Effect of Additional Amino Acids on the Cysteine-Xylose Maillard Reaction to Form Volatile Flavor Compounds[J]. Science and Technology of Food Industry, 2026 , 47 (13) : 262 -271 . DOI: 10.13386/j.issn1002-0306.2025040257
Year 2026 volume 47 Issue 13
180
77
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doi: 10.13386/j.issn1002-0306.2025040257
  • Receive Date:2025-04-20
  • Online Date:2026-10-24
  • Published:2026-07-01
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  • Received:2025-04-20
Affiliations
    School of Food and Health, Beijing Technology and Business University (BTBU), Key Laboratory of Geriatric Nutrition and Health, Ministry of Education, Beijing 100048, China
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表12种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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