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Shrimp shells are currently the primary source of chitin, and demineralization of shrimp shells is a key step in the preparation of chitin. In this study, two acid-producing strains, GXUN-XS3 and GXUN-XS8, were isolated from abandoned shrimp ponds. They were identified through morphological and molecular methods. The optimal fermentation conditions were optimized using single-factor and response surface experiments. SEM analysis was used to characterize the fermentation products. The results showed that the isolated strains GXUN-XS3 and GXUN-XS8 were identified as Enterococcus faecalis and Exiguobacterium aestuarii, respectively. The optimized GXUN-XS3 was fermented in a medium containing 2% shrimp shell powder, 3% glucose, and 1% sodium chloride at a temperature of 30.5 ℃, an initial pH of 5.6, and an inoculum size of 3.3% for 3 days. The demineralization of shrimp shell reached 96.09%, which was an increase of 6.29% compared to before optimization. The optimized GXUN-XS8 fermented in a medium containing 2% shrimp shell powder, 2% maltose, and 1% sodium chloride at a temperature of 30.7 ℃, an initial pH of 5.7, and an inoculum size of 3.9% for 4 days. The demineralization of shrimp shell reached 95.73%, which was an increase of 6.17% compared to before optimization. SEM analysis revealed that the products had a fibrous structure with pores, more intact than industrial chitin produced by acid-alkali methods. This study provides microbial resources and references for the microbial fermentation degradation of shrimp shell waste.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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