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Heterologous Expression of a Thermostable Arabinoxylan Specific Xylanase from Paecilomyces thermophila and Its Application in Malt Mashing
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Hang YANG1, Wenjiao ZHANG2, Xiemin YAN1, Wei ZHAO3, Zhengqiang JIANG2, Qiaojuan YAN1, *, Shaoqing YANG2, *
Science and Technology of Food Industry | 2026, 47(1) : 189 - 196
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Science and Technology of Food Industry | 2026, 47(1): 189-196
• Bioengineering •
Heterologous Expression of a Thermostable Arabinoxylan Specific Xylanase from Paecilomyces thermophila and Its Application in Malt Mashing
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Hang YANG1, Wenjiao ZHANG2, Xiemin YAN1, Wei ZHAO3, Zhengqiang JIANG2, Qiaojuan YAN1, *, Shaoqing YANG2, *
Affiliations
  • 1College of Engineering, China Agricultural University, Beijing 100083, China
  • 2College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • 3Shandong Fuyang Bio-tech. Co., Ltd., Dezhou 253199, China‌
Published: 2026-01-01 doi: 10.13386/j.issn1002-0306.2024100013
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Objective: This study aimed to identify thermostable xylanase suitable for mash malting. Methods: A xylanase encoding gene (PtXynA) from Paecilomyces thermophila J18 was cloned and heterologously expressed in Aspergillus niger. A submerged fermentation in a 5 L fermentor was performed for the production of the recombinant enzyme (PtXynA). The enzymatic properties of PtXynA were characterized and its application potential in mash malting was further evaluated. Results: A maximum extracellular xylanase activity of 904.0 U/mL with a protein content of 1.4 mg/mL was obtained after 96 h of fermentation. PyXynA was most active at pH7.0 and 70 ℃, respectively, and it was stable within the pH range of 5.5~9.0 and at temperatures below 75 ℃. The enzyme displayed strict substrate specificity towards various xylans, with the highest specific activity towards wheat arabinoxylan (1993.2 U/mg), followed by oat spelt xylan (1782.5 U/mg), beechwood xylan (1552.0 U/mg) and birchwood xylan (1238.7 U/mg). The addition of PtXynA in mash (200 U/g malt) reduced the filtration time and viscosity of malt by 43.9% and 10.8%, respectively. Conclusion: The excellent properties may enable PyXynA great application potential in beer industry.

Paecilomyces thermophila  /  heterologous expression  /  thermophilic xylanase  /  arabinoxylanase  /  mash malting
杨行, 张文姣, 闫协民, 赵伟, 江正强, 闫巧娟, 杨绍青. 嗜热拟青霉耐热阿拉伯木聚糖酶的异源表达及其对麦芽糖化的影响. 食品工业科技, 2026 , 47 (1) : 189 -196 . DOI: 10.13386/j.issn1002-0306.2024100013
Hang YANG, Wenjiao ZHANG, Xiemin YAN, Wei ZHAO, Zhengqiang JIANG, Qiaojuan YAN, Shaoqing YANG. Heterologous Expression of a Thermostable Arabinoxylan Specific Xylanase from Paecilomyces thermophila and Its Application in Malt Mashing[J]. Science and Technology of Food Industry, 2026 , 47 (1) : 189 -196 . DOI: 10.13386/j.issn1002-0306.2024100013
Appendix
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Year 2026 volume 47 Issue 1
119
45
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Article Info
doi: 10.13386/j.issn1002-0306.2024100013
  • Receive Date：2024-10-08
  • Online Date：2026-10-24
  • Published：2026-01-01
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  • Received：2024-10-08
Affiliations
    1College of Engineering, China Agricultural University, Beijing 100083, China
    2College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
    3Shandong Fuyang Bio-tech. Co., Ltd., Dezhou 253199, China‌
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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