Article(id=1320881272558306118, tenantId=1146029695717560320, journalId=1260987677001138203, issueId=1320880625310085394, articleNumber=null, orderNo=null, doi=10.13386/j.issn1002-0306.2025090242, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1758470400000, receivedDateStr=2025-09-22, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1792852236165, onlineDateStr=2026-10-24, pubDate=1788192000000, pubDateStr=2026-09-01, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1792852236165, onlineIssueDateStr=2026-10-24, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1792852236165, creator=13701087609, updateTime=1792852236165, updator=13701087609, issue=Issue{id=1320880625310085394, tenantId=1146029695717560320, journalId=1260987677001138203, year='2026', volume='47', issue='17', pageStart='1', pageEnd='596', issueExtLink='null', onlineDate='null', pubDate='1788192000000', pubDateStr='2026-09-01', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1792852081848, creator='13701087609', updateTime=1792852081848, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=233, endPage=243, ext={EN=ArticleExt(id=1320881272755438407, articleId=1320881272558306118, tenantId=1146029695717560320, journalId=1260987677001138203, language=EN, title=Growth Kinetics and Organoleptic Characterization of Bacillus cereus in Three Different Dairy Products, columnId=1261336275769016441, journalTitle=Science and Technology of Food Industry, columnName=Bioengineering, runingTitle=null, highlight=null, articleAbstract=

The purpose of this study was to investigate the growth kinetics of Bacillus cereus in different milk products and its impact on milk quality. Fifty dairy products (including 25 raw milk from factory pre-treatment tanks and 25 commercial milk packaged in boxes) were collected, and suspected strains were isolated using Mannitol-Egg-Yolk-Polymyxin Agar (MYP). Morphological observation, protein toxin crystallization experiment, Vitek-2 biochemical identification, and 16S rRNA sequencing were used to isolate and identify B. cereus. Further, one strain with the fastest growth rate was selected as the experimental object, and the strain was inoculated into three different milk products (pure milk, lactose-decomposed milk, and grain milk) at initial concentrations of 101, 102, and 103 CFU/mL. The growth kinetics of B. cereus in three different products were studied at two temperatures (25 ℃ and 35 ℃), and a Logistic equation was used to fit the growth model. The pH and sensory changes of the three milk products were simultaneously monitored. The results showed that the three strains (B1, B2, and B3) of B. cereus originated from raw milk were isolated and identified and they were not detected in commercial milk products. By measuring the growth curve, B1 strain with a faster growth rate was selected as the experimental object to study its effects on three different milk products. The results showed that temperature and initial inoculation concentration had a more significant impact on the growth of B. cereus. At 35 ℃ and high initial inoculation concentration, B. cereus in dairy products entered the logarithmic and stable phases earlier than at 25 ℃, with a shorter lag period. Among the three types of products, lactose-decomposed milk was more conducive to the growth of B. cereus. The fitting degree of the Logistic model was R2>0.9857. It was found that the pH of milk changed significantly as the bacterial concentration increased. Different dairy products had their own characteristics in terms of sensory degradation. Among them, the odor deterioration of lactose-decomposed milk was the most significant. This study provides a scientific basis for the early warning and safety control of B. cereus contamination in dairy products.

, authors=Qiangqiang CHEN1, Xiaodi KOU1, Haijun XU1, Yu HU2, Junni TANG1, *, authorsList=Qiangqiang CHEN, Xiaodi KOU, Haijun XU, Yu HU, Junni TANG, authorCompany=null, correspAuthors=Junni TANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=Copyright © 2026 Science and Technology of Food Industry. All rights reserved., copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1320881276291236696, articleId=1320881272558306118, tenantId=1146029695717560320, journalId=1260987677001138203, language=CN, title=蜡样芽孢杆菌在三种不同乳产品中的生长动力学特性以及引起乳制品感官变化特征, columnId=1261336277547401341, journalTitle=食品工业科技, columnName=生物工程, runingTitle=null, highlight=null, articleAbstract=

为探究蜡样芽孢杆菌在不同牛奶产品中的生长动力学特性及对牛奶品质影响,本研究采集了50份乳制品(包括25份工厂前处理罐原料乳和25份市售纸盒包装商品乳),通过甘露醇卵黄多粘菌素琼脂培养基(MYP)分离疑似菌株,结合形态学观察、蛋白质毒素结晶实验、Vitek-2生化鉴定及16S rRNA测序分离鉴定蜡样芽孢杆菌;以筛选生长速率最快的菌株作为实验对象,按101、102、103 CFU/mL初始浓度回接至三种不同牛奶产品(纯牛奶、舒化牛奶和谷粒牛奶)中,研究在两种温度(25 ℃和35 ℃)下,蜡样芽孢杆菌在三种不同产品中的生长动力学,采用Logistic方程拟合生长模型,同步监测三种牛奶产品的pH变化和感官影响。结果显示:分离鉴定的3株蜡样芽孢杆菌(B1、B2和B3)均源自原料乳,市售乳中未检出;通过生长曲线测定,研究B1菌株对三种不同牛奶产品的影响,结果表明温度和初始接种浓度对蜡样芽孢杆菌的生长影响较为明显,在35 ℃条件下和高初始接种浓度下,乳制品中的蜡样芽孢杆菌较25 ℃更早进入对数期和稳定期,迟滞期更短,三种类型的产品中,舒化牛奶更有利于蜡样芽孢杆菌的生长;Logistic模型拟合度R2>0.9857;牛奶的pH随菌浓度增长变化明显,不同乳制品感官劣变具有各自的特异性,其中舒化牛奶气味劣变最为显著。本研究为乳制品蜡样芽孢杆菌污染预警及安全控制提供科学依据

, authors=陈强强1, 寇肖迪1, 徐海俊1, 胡瑜2, 唐俊妮1, *, authorsList=陈强强, 寇肖迪, 徐海俊, 胡瑜, 唐俊妮, authorCompany=null, correspAuthors=唐俊妮, authorNote=

陈强强(1999−),女,硕士研究生,研究方向:食品加工与安全,E-mail:

, correspAuthorsNote=
唐俊妮(1971−),女,博士,教授,研究方向:食品微生物与食品安全,E-mail:。
, copyrightStatement=版权所有 © 2026《食品工业科技》编辑部, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=XaxV9f3spPp02ZBIaFTEQw==, magXml=sxT9sddLgueCBKpf/TFpqw==, pdfUrl=null, pdf=yifPX55AUFTbiKYC6fi5Cw==, pdfFileSize=8604060, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=NG7dVFvA8uxHYYlNhewcbg==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=7kHwPgQ5q1mkCRxSovoBzw==, mapNumber=null, fund=null)}, authors=[Author(id=1320881579589750798, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=2338659742@qq.com, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320881579661053968, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881579589750798, language=EN, stringName=Qiangqiang CHEN, firstName=Qiangqiang, middleName=null, lastName=CHEN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320881579723968529, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881579589750798, language=CN, stringName=陈强强, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1西南民族大学药学与食品学院,四川成都 610225, bio={"content":"

陈强强(1999−),女,硕士研究生,研究方向:食品加工与安全,E-mail:

"}, bioImg=null, bioContent=

陈强强(1999−),女,硕士研究生,研究方向:食品加工与安全,E-mail:

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320881579409395719, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, xref=1, ext=[AuthorCompanyExt(id=1320881579417784328, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China), AuthorCompanyExt(id=1320881579421978633, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1西南民族大学药学与食品学院,四川成都 610225)])]), Author(id=1320881579803660307, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320881579874963477, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881579803660307, language=EN, stringName=Xiaodi KOU, firstName=Xiaodi, middleName=null, lastName=KOU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320881579946266646, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881579803660307, language=CN, stringName=寇肖迪, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1西南民族大学药学与食品学院,四川成都 610225, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320881579409395719, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, xref=1, ext=[AuthorCompanyExt(id=1320881579417784328, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China), AuthorCompanyExt(id=1320881579421978633, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1西南民族大学药学与食品学院,四川成都 610225)])]), Author(id=1320881580004986904, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320881580072095770, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881580004986904, language=EN, stringName=Haijun XU, firstName=Haijun, middleName=null, lastName=XU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320881580151787547, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881580004986904, language=CN, stringName=徐海俊, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1西南民族大学药学与食品学院,四川成都 610225, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320881579409395719, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, xref=1, ext=[AuthorCompanyExt(id=1320881579417784328, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China), AuthorCompanyExt(id=1320881579421978633, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1西南民族大学药学与食品学院,四川成都 610225)])]), Author(id=1320881580340531229, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320881580453777439, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881580340531229, language=EN, stringName=Yu HU, firstName=Yu, middleName=null, lastName=HU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2SIG Combibloc (Suzhou) Co., Ltd., Suzhou 215021, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320881580533469216, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881580340531229, language=CN, stringName=胡瑜, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2康美包(苏州)有限公司,江苏苏州 215021, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320881579493281802, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, xref=2, ext=[AuthorCompanyExt(id=1320881579501670411, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579493281802, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2SIG Combibloc (Suzhou) Co., Ltd., Suzhou 215021, China), AuthorCompanyExt(id=1320881579510059020, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579493281802, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2康美包(苏州)有限公司,江苏苏州 215021)])]), Author(id=1320881580617355298, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=392783291@qq.com, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1320881580688658468, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881580617355298, language=EN, stringName=Junni TANG, firstName=Junni, middleName=null, lastName=TANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, *, address=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320881580764155941, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881580617355298, language=CN, stringName=唐俊妮, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, *, address=1西南民族大学药学与食品学院,四川成都 610225, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320881579409395719, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, xref=1, ext=[AuthorCompanyExt(id=1320881579417784328, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China), AuthorCompanyExt(id=1320881579421978633, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1西南民族大学药学与食品学院,四川成都 610225)])])], keywords=[Keyword(id=1320881580906762278, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, orderNo=1, keyword=Bacillus cereus), Keyword(id=1320881580999036967, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, orderNo=2, keyword=dairy products), Keyword(id=1320881581061951528, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, orderNo=3, keyword=growth kinetics), Keyword(id=1320881584614527017, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, orderNo=4, keyword=pH changes), Keyword(id=1320881584677441578, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, orderNo=5, keyword=sensory quality), Keyword(id=1320881584740356139, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, orderNo=1, keyword=蜡样芽孢杆菌), Keyword(id=1320881584790687788, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, orderNo=2, keyword=乳品), Keyword(id=1320881584874573869, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, orderNo=3, keyword=生长动力学), Keyword(id=1320881584950071342, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, orderNo=4, keyword=pH变化), Keyword(id=1320881585046540335, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, orderNo=5, keyword=感官品质)], refs=[Reference(id=1320881588683001928, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=1, rfOrder=0, authorNames=null, journalName=null, refType=null, unstructuredReference=OECD-FAO agricultural outlook 2022-2031[M]. Paris: OECD Publishing, 2022., articleTitle=null, refAbstract=null), Reference(id=1320881588745916489, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=2, rfOrder=1, authorNames=null, journalName=null, refType=null, unstructuredReference=中国乳制品工业协会. 2023中国乳制品行业质量安全报告[R]. 北京: 中国乳制品工业协会, 2024. [China Dairy Industry Association. 2023 China dairy industry quality and safety report[R]. Beijing: China Dairy Industry Association, 2024.], articleTitle=null, refAbstract=null), Reference(id=1320881588863357002, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=2, rfOrder=2, authorNames=null, journalName=null, refType=null, unstructuredReference=China Dairy Industry Association. 2023 China dairy industry quality and safety report[R]. Beijing: China Dairy Industry Association, 2024., articleTitle=null, refAbstract=null), Reference(id=1320881589148569675, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=3, rfOrder=3, authorNames=null, journalName=null, refType=null, unstructuredReference=TIRLONI E, STELLA S, CELANDRONI F, et al. Bacillus cereus in dairy products and production plants[J]. Foods, 2022, 11(17): 1−12., articleTitle=null, refAbstract=null), Reference(id=1320881589215678540, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=4, rfOrder=4, authorNames=null, journalName=null, refType=null, unstructuredReference=郭佳, 王娉, 周继福, 等. 乳源蜡样芽孢杆菌耐药性、毒力因子检测及分子特征研究[J]. 中国农业科技导报, 2021, 23(11): 131−138. [GUO J, WANG P, ZHOU J F, et al. Study on drug resistance and molecular typing of Bacillus cereus from dairy products[J]. China Agricultural Science and Technology Herald, 2021, 23(11): 131−138.], articleTitle=null, refAbstract=null), Reference(id=1320881589278593101, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=4, rfOrder=5, authorNames=null, journalName=null, refType=null, unstructuredReference=GUO J, WANG P, ZHOU J F, et al. Study on drug resistance and molecular typing of Bacillus cereus from dairy products[J]. China Agricultural Science and Technology Herald, 2021, 23(11): 131−138., articleTitle=null, refAbstract=null), Reference(id=1320881589345701966, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=5, rfOrder=6, authorNames=null, journalName=null, refType=null, unstructuredReference=LOGAN N A V P D. Bergey's manual of systematics of archaea and bacteria[M]. Hoboken, New Jersey: John Wiley & Sons, 2015: 1-163., articleTitle=null, refAbstract=null), Reference(id=1320881589408616527, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=6, rfOrder=7, authorNames=null, journalName=null, refType=null, unstructuredReference=CEUPPENS S, BOON N, UYTTENDAELE M. Diversity of Bacillus cereus group strains is reflected in their broad range of pathogenicity and diverse ecological lifestyles[J]. Fems Microbiology Ecology, 2013, 84(3): 433−450., articleTitle=null, refAbstract=null), Reference(id=1320881589484114000, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=7, rfOrder=8, authorNames=null, journalName=null, refType=null, unstructuredReference=AUTHORITY E E F S. Scientific opinion on the public health risks of bacterial toxins in dairy products[J]. EFSA Journal, 2016, 14(1): 4362., articleTitle=null, refAbstract=null), Reference(id=1320881589551222865, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=8, rfOrder=9, authorNames=null, journalName=null, refType=null, unstructuredReference=LE MARC Y, BUSS DA SILVA N, POSTOLLEC F, et al. A stochastic approach for modelling the effects of temperature on the growth rate of Bacillus cereus sensu lato[J]. International Journal of Food Microbiology, 2021, 349: 109241., articleTitle=null, refAbstract=null), Reference(id=1320881589626720338, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=9, rfOrder=10, authorNames=null, journalName=null, refType=null, unstructuredReference=郑跃, 赵鹏, 谢翔燕, 等. 国内外食品中蜡样芽孢杆菌污染情况及控制研究[J]. 食品与发酵工业, 2026, 52(9): 402−410. [ZHENG Yue, ZHAO Peng, XIE Xiangyan, et al. Progress of the contamination and control of Bacillus cereus in foods at home andabroad[J]. Food and Fermentation Industries, 2026, 52(9): 402−410.], articleTitle=null, refAbstract=null), Reference(id=1320881589689634899, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=9, rfOrder=11, authorNames=null, journalName=null, refType=null, unstructuredReference=ZHENG Yue, ZHAO Peng, XIE Xiangyan, et al. Progress of the contamination and control of Bacillus cereus in foods at home andabroad[J]. Food and Fermentation Industries, 2026, 52(9): 402−410., articleTitle=null, refAbstract=null), Reference(id=1320881589739966548, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=10, rfOrder=12, authorNames=null, journalName=null, refType=null, unstructuredReference=ZHAO S, CHEN J, FEI P, et al. Prevalence, molecular characterization, and antibiotic susceptibility of Bacillus cereus isolated from dairy products in China[J]. Journal of Dairy Science, 2020, 103(5): 3994−4001., articleTitle=null, refAbstract=null), Reference(id=1320881589815464021, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=11, rfOrder=13, authorNames=null, journalName=null, refType=null, unstructuredReference=MAKTABDAR M, HOUMANN R H, SCHEEL N H, et al. Evaluation and validation of extensive growth and growth boundary models for mesophilic and psychrotolerant Bacillus cereus in dairy products (Part 2)[J]. Frontiers in Microbiology, 2025, 16: 1553903., articleTitle=null, refAbstract=null), Reference(id=1320881589895155798, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=12, rfOrder=14, authorNames=null, journalName=null, refType=null, unstructuredReference=MEHTA D S, METZGER L E, HASSAN A N, et al. The ability of spore formers to degrade milk proteins, fat, phospholipids, common stabilizers, and exopolysaccharides[J]. Journal of Dairy Science, 2019, 102(12): 10799−10813., articleTitle=null, refAbstract=null), Reference(id=1320881589983236183, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=13, rfOrder=15, authorNames=null, journalName=null, refType=null, unstructuredReference=CARRASCOSA C, RAHEEM D, RAMOS F, et al. Microbial biofilms in the food industry-A comprehensive review[J]. International Journal of Environmental Research and Public Health, 2021, 18(4): 2014., articleTitle=null, refAbstract=null), Reference(id=1320881590092288088, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=14, rfOrder=16, authorNames=null, journalName=null, refType=null, unstructuredReference=仪淑敏, 张培正, 李远钊, 等. 蜡样芽孢杆菌在营养肉汤中的生长模型[J]. 食品研究与开发, 2009, 30(9): 69−72. [YI S M, ZHANG P Z, LI Y Z, et al. Growth of Bacillus cereus in nutritional broth[J]. Food Research and Development, 2009, 30(9): 69−72.], articleTitle=null, refAbstract=null), Reference(id=1320881590163591257, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=14, rfOrder=17, authorNames=null, journalName=null, refType=null, unstructuredReference=YI S M, ZHANG P Z, LI Y Z, et al. Growth of Bacillus cereus in nutritional broth[J]. Food Research and Development, 2009, 30(9): 69−72., articleTitle=null, refAbstract=null), Reference(id=1320881590239088730, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=15, rfOrder=18, authorNames=null, journalName=null, refType=null, unstructuredReference=RAUH V M, JOHANSEN L B, IPSEN R, et al. Plasmin activity in UHT milk: Relationship between proteolysis, age gelation, and bitterness[J]. Journal of Agricultural and Food Chemistry, 2014, 62(28): 6852−6860., articleTitle=null, refAbstract=null), Reference(id=1320881590310391899, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=16, rfOrder=19, authorNames=null, journalName=null, refType=null, unstructuredReference=王慧莹. 基于计算机视觉技术快速检测食品中蜡样芽孢杆菌的研究[D]. 长春: 吉林大学, 2011. [WANG H Y. Research on rapid detection of Bacillus cereus in food by computer vision[D]. Changchun: Jilin University, 2011.], articleTitle=null, refAbstract=null), Reference(id=1320881590369112156, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=16, rfOrder=20, authorNames=null, journalName=null, refType=null, unstructuredReference=WANG H Y. Research on rapid detection of Bacillus cereus in food by computer vision[D]. Changchun: Jilin University, 2011., articleTitle=null, refAbstract=null), Reference(id=1320881590440415325, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=17, rfOrder=21, authorNames=null, journalName=null, refType=null, unstructuredReference=YANG Y, WEI S, ZHONG J, et al. Exposure assessment of Bacillus cereus introduced into reconstituted milk production in China[J]. Food Bioscience, 2024, 59: 104024., articleTitle=null, refAbstract=null), Reference(id=1320881590520107102, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=18, rfOrder=22, authorNames=null, journalName=null, refType=null, unstructuredReference=牛犇, 洪斌, 穆丽丽, 等. 荧光定量PCR在预测微生物学中的应用[J]. 食品工业科技, 2019, 40(5): 275−282. [NIU B, HONG B, MU L L, et al. Application of fluorescent quantitative PCR in predictive microbiology[J]. Science and Technology of Food Industry, 2019, 40(5): 275−282.], articleTitle=null, refAbstract=null), Reference(id=1320881590645936223, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=18, rfOrder=23, authorNames=null, journalName=null, refType=null, unstructuredReference=NIU B, HONG B, MU L L, et al. Application of fluorescent quantitative PCR in predictive microbiology[J]. Science and Technology of Food Industry, 2019, 40(5): 275−282., articleTitle=null, refAbstract=null), Reference(id=1320881590700462176, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=19, rfOrder=24, authorNames=null, journalName=null, refType=null, unstructuredReference=RAUH V M I R D E. Plasmin and bacterial protease interactions in UHT milk during storage[J]. Journal of Agricultural and Food Chemistry, 2020, 68(12): 3564−3572., articleTitle=null, refAbstract=null), Reference(id=1320881590788542561, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=20, rfOrder=25, authorNames=null, journalName=null, refType=null, unstructuredReference=屈雅莉, 张彦辉, 夏兆田, 等. 分离自生牛乳的一株高产蛋白酶蜡样芽孢杆菌特性[J]. 乳业科学与技术, 2025, 48(3): 55−62. [QU Y L, ZHANG Y H, XIA Z T, et al. Characteristics of a protease-producing strain of Bacillus cereus isolated from raw milk[J]. Journal of Dairy Science and Technology, 2025, 48(3): 55−62.], articleTitle=null, refAbstract=null), Reference(id=1320881590893400162, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=20, rfOrder=26, authorNames=null, journalName=null, refType=null, unstructuredReference=QU Y L, ZHANG Y H, XIA Z T, et al. Characteristics of a protease-producing strain of Bacillus cereus isolated from raw milk[J]. Journal of Dairy Science and Technology, 2025, 48(3): 55−62., articleTitle=null, refAbstract=null), Reference(id=1320881590968897635, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=21, rfOrder=27, authorNames=null, journalName=null, refType=null, unstructuredReference=黄立波. VITEK-2全自动微生物鉴定仪在蜡样芽孢杆菌检测中应用分析[J]. 中国医疗器械信息, 2021, 27(6): 42−43. [HUANG L B. VITEK-2 automatic microbial identification instrument was applied in the detection of Bacillus cereus[J]. Information on Chinese medical devices, 2021, 27(6): 42−43.], articleTitle=null, refAbstract=null), Reference(id=1320881591044395108, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=21, rfOrder=28, authorNames=null, journalName=null, refType=null, unstructuredReference=HUANG L B. VITEK-2 automatic microbial identification instrument was applied in the detection of Bacillus cereus[J]. Information on Chinese medical devices, 2021, 27(6): 42−43., articleTitle=null, refAbstract=null), Reference(id=1320881592722116709, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=22, rfOrder=29, authorNames=null, journalName=null, refType=null, unstructuredReference=中华人民共和国国家卫生和计划生育委员会. 食品安全国家标准 食品微生物学检验 蜡样芽孢杆菌检验: GB 4789.14-2014[S]. 北京: 中国标准出版社, 2014. [National Health and Family Planning Commission of the People's Republic of China. National food safety standard: Microbiological examination of food-Bacillus cereus test: GB 4789.14-2014 [S]. Beijing: China Standards Press, 2014.], articleTitle=null, refAbstract=null), Reference(id=1320881592797614182, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=22, rfOrder=30, authorNames=null, journalName=null, refType=null, unstructuredReference=National Health and Family Planning Commission of the People's Republic of China. National food safety standard: Microbiological examination of food-Bacillus cereus test: GB 4789.14-2014 [S]. Beijing: China Standards Press, 2014., articleTitle=null, refAbstract=null), Reference(id=1320881592868917351, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=23, rfOrder=31, authorNames=null, journalName=null, refType=null, unstructuredReference=廖丽辉, 侯艳飞, 秦捷, 等. 蜡样芽孢杆菌根状生长实验方法探讨[J]. 现代食品, 2023, 29(13): 144−147. [LIAO L H, HOU Y F, QIN J, et al. Discussion on the experimental method of Bacillus cereus rhizoid growth[J]. Modern Food, 2023, 29(13): 144−147.], articleTitle=null, refAbstract=null), Reference(id=1320881592931831912, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=23, rfOrder=32, authorNames=null, journalName=null, refType=null, unstructuredReference=LIAO L H, HOU Y F, QIN J, et al. Discussion on the experimental method of Bacillus cereus rhizoid growth[J]. Modern Food, 2023, 29(13): 144−147., articleTitle=null, refAbstract=null), Reference(id=1320881592998940777, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=24, rfOrder=33, authorNames=null, journalName=null, refType=null, unstructuredReference=凌云, 王晶, 王越菲, 等. 不同裂解方式对微生物DNA提取的影响[J]. 江苏农业科学, 2016, 44(12): 61−64. [LING Y, WANG J, WANG Y F, et al. The impact of different lysis methods on microbial DNA extraction[J]. Jiangsu Agricultural Science, 2016, 44(12): 61−64.], articleTitle=null, refAbstract=null), Reference(id=1320881593066049642, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=24, rfOrder=34, authorNames=null, journalName=null, refType=null, unstructuredReference=LING Y, WANG J, WANG Y F, et al. The impact of different lysis methods on microbial DNA extraction[J]. Jiangsu Agricultural Science, 2016, 44(12): 61−64., articleTitle=null, refAbstract=null), Reference(id=1320881593158324331, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=25, rfOrder=35, authorNames=null, journalName=null, refType=null, unstructuredReference=张鑫, 罗雨帆, 许庆方. 一株芽孢杆菌的筛选及其降解木质纤维素性能[J]. 微生物学报, 2025, 65(4): 1512−1528. [ZHANG X, LUO Y F, XU Q F. A Bacillus strain capable of degrading lignocellulose[J]. Acta Microbiologica Sinica, 2025, 65(4): 1512−1528.], articleTitle=null, refAbstract=null), Reference(id=1320881593242210412, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=25, rfOrder=36, authorNames=null, journalName=null, refType=null, unstructuredReference=ZHANG X, LUO Y F, XU Q F. A Bacillus strain capable of degrading lignocellulose[J]. Acta Microbiologica Sinica, 2025, 65(4): 1512−1528., articleTitle=null, refAbstract=null), Reference(id=1320881593317707885, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=26, rfOrder=37, authorNames=null, journalName=null, refType=null, unstructuredReference=ELAHI S, FUJIKAWA H. Comprehensive study of the boundaries of enterotoxin A production and growth of Staphylococcus aureus at various temperatures and salt concentrations[J]. Journal of Food Science, 2019, 84(1): 121−126., articleTitle=null, refAbstract=null), Reference(id=1320881593384816750, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=27, rfOrder=38, authorNames=null, journalName=null, refType=null, unstructuredReference=陈琛, 李学英, 杨宪时, 等. 环境因子交互作用下蜡样芽孢杆菌生长/非生长界面模型的建立与评价[J]. 现代食品科技, 2015, 31(12): 205−213. [CHEN C, LI X Y, YANG X S, et al. Modeling and evaluating the growth/no growth boundaries of Bacillus cereus: Effect of temperature, pH, and water activity[J]. Modern Food Science & Technology, 2015, 31(12): 205−213.], articleTitle=null, refAbstract=null), Reference(id=1320881593451925615, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=27, rfOrder=39, authorNames=null, journalName=null, refType=null, unstructuredReference=CHEN C, LI X Y, YANG X S, et al. Modeling and evaluating the growth/no growth boundaries of Bacillus cereus: Effect of temperature, pH, and water activity[J]. Modern Food Science & Technology, 2015, 31(12): 205−213., articleTitle=null, refAbstract=null), Reference(id=1320881593523228784, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=28, rfOrder=40, authorNames=null, journalName=null, refType=null, unstructuredReference=MEMBRE J, LAMBERT R J W. Application of predictive modelling techniques in industry: from food design up to risk assessment[J]. International Journal of Food Microbiology, 2008, 128(1): 10−15., articleTitle=null, refAbstract=null), Reference(id=1320881593581949041, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=29, rfOrder=41, authorNames=null, journalName=null, refType=null, unstructuredReference=中华人民共和国卫生部. 食品安全国家标准 生乳: GB 19301-2010[S]. 北京: 中国标准出版社, 2010. [Ministry of Health of the People's Republic of China. National food safety standards for raw milk: GB 19301-2010[S]. Beijing: China Standards Press, 2010.], articleTitle=null, refAbstract=null), Reference(id=1320881593644863602, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=29, rfOrder=42, authorNames=null, journalName=null, refType=null, unstructuredReference=Ministry of Health of the People's Republic of China. National food safety standards for raw milk: GB 19301-2010[S]. Beijing: China Standards Press, 2010., articleTitle=null, refAbstract=null), Reference(id=1320881593707778163, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=30, rfOrder=43, authorNames=null, journalName=null, refType=null, unstructuredReference=邵景海, 王占东, 王青云, 等. 双蛋白有机酸奶产品的开发[J]. 食品安全导刊, 2021(22): 93−95. [SHAO J H, WANG Z D, WANG Q Y, et al. Development of double-protein organic yogurt products[J]. China Food Safety Magazine, 2021(22): 93−95.], articleTitle=null, refAbstract=null), Reference(id=1320881593766498420, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=30, rfOrder=44, authorNames=null, journalName=null, refType=null, unstructuredReference=SHAO J H, WANG Z D, WANG Q Y, et al. Development of double-protein organic yogurt products[J]. China Food Safety Magazine, 2021(22): 93−95., articleTitle=null, refAbstract=null), Reference(id=1320881593829412981, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=31, rfOrder=45, authorNames=null, journalName=null, refType=null, unstructuredReference=乔霖楠, 刘阳泰, 王晔茹, 等. 中国食品中蜡样芽孢杆菌污染状况荟萃分析[J]. 工业微生物, 2025, 55(1): 155−160. [QIAO L N, LIU Y T, WANG Y R, et al. A meta-analysis of Bacillus cereus prevalence in Chinese foods[J]. Industrial Microbiology, 2025, 55(1): 155−160.], articleTitle=null, refAbstract=null), Reference(id=1320881593896521846, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=31, rfOrder=46, authorNames=null, journalName=null, refType=null, unstructuredReference=QIAO L N, LIU Y T, WANG Y R, et al. A meta-analysis of Bacillus cereus prevalence in Chinese foods[J]. Industrial Microbiology, 2025, 55(1): 155−160., articleTitle=null, refAbstract=null), Reference(id=1320881593967825015, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=32, rfOrder=47, authorNames=null, journalName=null, refType=null, unstructuredReference=BARANYI J, ROBERTS T A. Mathematics of predictive food microbiology[J]. International Journal of Food Microbiology, 1995, 26(2): 199−218., articleTitle=null, refAbstract=null), Reference(id=1320881594043322488, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=33, rfOrder=48, authorNames=null, journalName=null, refType=null, unstructuredReference=KYRITSI M, TSIOLAS G, TSOUREKI A, et al. Genomic and transcriptomic profiling of Bacillus cereus in milk: Insights into the sweet curdling defect[J]. Foods, 2025, 14(5): 780., articleTitle=null, refAbstract=null), Reference(id=1320881594127208569, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=34, rfOrder=49, authorNames=null, journalName=null, refType=null, unstructuredReference=AGUILAR C E G, ROSSI G A M, SILVA H O, et al. Gene detection and enzymatic activity of psychrotrophic Bacillus cereus s. isolated from milking environments, dairies, milk, and dairy products[J]. Microorganisms, 2025, 13(4): 889., articleTitle=null, refAbstract=null)], funds=null, companyList=[AuthorCompany(id=1320881579409395719, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, xref=1, ext=[AuthorCompanyExt(id=1320881579417784328, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China), AuthorCompanyExt(id=1320881579421978633, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1西南民族大学药学与食品学院,四川成都 610225)]), AuthorCompany(id=1320881579493281802, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, xref=2, ext=[AuthorCompanyExt(id=1320881579501670411, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579493281802, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2SIG Combibloc (Suzhou) Co., Ltd., Suzhou 215021, China), AuthorCompanyExt(id=1320881579510059020, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579493281802, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2康美包(苏州)有限公司,江苏苏州 215021)])], figs=[ArticleFig(id=1320881585231089712, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, label=Fig.1, caption=Morphology of three isolated strains on MYP medium and Gram staining microscopy results (B1, B2, and B3), figureFileSmall=B7khhhEuytMn76TzzllT/Q==, figureFileBig=NG7dVFvA8uxHYYlNhewcbg==, tableContent=null), ArticleFig(id=1320881585298198577, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, label=图1, caption=三株分离菌株在MYP培养基上的形态特征和革兰染色镜检结果(B1、B2和B3)

注:油镜观察,放大倍数1000×。

, figureFileSmall=B7khhhEuytMn76TzzllT/Q==, figureFileBig=NG7dVFvA8uxHYYlNhewcbg==, tableContent=null), ArticleFig(id=1320881585407250482, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, label=Fig.2, caption=Results for B. cereus rhizoid growth experiment, figureFileSmall=EJbxnNuDTb5ubRpgBg25fA==, figureFileBig=wKwAojtRKYGGIc31srT5Nw==, tableContent=null), ArticleFig(id=1320881585482747955, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, label=图2, caption=蜡样芽孢杆菌根状生长实验

注:左边为蕈状芽孢杆菌;B1、B2、B3分别为筛选出的三株蜡样芽孢杆菌菌落生长形态。

, figureFileSmall=EJbxnNuDTb5ubRpgBg25fA==, figureFileBig=wKwAojtRKYGGIc31srT5Nw==, tableContent=null), ArticleFig(id=1320881585558245428, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, label=Fig.3, caption=Protein toxin crystallization experiment, figureFileSmall=U9F0QlL6Xk+SbEqC1keJ2A==, figureFileBig=aAlgSKo4Yh8WhP/wtuNN6w==, tableContent=null), ArticleFig(id=1320881585612771381, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, label=图3, caption=蛋白质毒素结晶实验

注:从左到右分别为苏云金芽孢杆菌、B1、B2和B3三株筛选的菌株,油镜观察,放大倍数1000×。

, figureFileSmall=U9F0QlL6Xk+SbEqC1keJ2A==, figureFileBig=aAlgSKo4Yh8WhP/wtuNN6w==, tableContent=null), ArticleFig(id=1320881585675685942, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, label=Fig.4, caption=Phylogenetic tree of 16S rRNA for three isolated strains, figureFileSmall=Fm/7XNSwepz3xxrnJs+Rag==, figureFileBig=fk5EmU/NoFIHyeNO/cLhAw==, tableContent=null), ArticleFig(id=1320881585751183415, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, label=图4, caption=三株分离菌株的16S rRNA系统发育进化树, figureFileSmall=Fm/7XNSwepz3xxrnJs+Rag==, figureFileBig=fk5EmU/NoFIHyeNO/cLhAw==, tableContent=null), ArticleFig(id=1320881585835069496, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, label=Fig.5, caption=Growth curves of three isolated B. cereus strains in pure milk at 25 ℃, figureFileSmall=/05inFaHlAq1BEu2B4GSgg==, figureFileBig=2m9BgoMcDt6o9Yle3AtckA==, tableContent=null), ArticleFig(id=1320881585897984057, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, label=图5, caption=25 ℃下三株分离的蜡样芽孢杆菌在纯牛奶中的生长曲线, figureFileSmall=/05inFaHlAq1BEu2B4GSgg==, figureFileBig=2m9BgoMcDt6o9Yle3AtckA==, tableContent=null), ArticleFig(id=1320881585969287226, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, label=Fig.6, caption=Growth curves of B. cereus B1 in pure milk (A), lactose-decomposed milk (B) and grain milk (C) at 25 ℃ and 35 ℃, figureFileSmall=LDexWIDpopPoNqxDofUOhw==, figureFileBig=WvI6z0vCu5ma7vZA5jHDZQ==, tableContent=null), ArticleFig(id=1320881586036396091, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, label=图6, caption=25 ℃和35 ℃下蜡样芽孢杆菌B1在纯牛奶(A)、舒化牛奶(B)和谷粒牛奶(C)中的生长曲线, figureFileSmall=LDexWIDpopPoNqxDofUOhw==, figureFileBig=WvI6z0vCu5ma7vZA5jHDZQ==, tableContent=null), ArticleFig(id=1320881586111893564, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, label=Fig.7, caption=Changes of pH due to the growth of B. cereus B1 in pure milk (A), lactose-decomposed milk (B) and grain milk (C) at 25 ℃ and 35 ℃, figureFileSmall=fI4CIU0Ak5SOf8F1lEgm1g==, figureFileBig=nw0SeH/ISMeQt2CsPXBaXA==, tableContent=null), ArticleFig(id=1320881586179002429, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, label=图7, caption=25 ℃和35 ℃下蜡样芽孢杆菌B1在纯牛奶(A)、舒化牛奶(B)和谷粒牛奶(C)中生长引起牛奶pH变化, figureFileSmall=fI4CIU0Ak5SOf8F1lEgm1g==, figureFileBig=nw0SeH/ISMeQt2CsPXBaXA==, tableContent=null), ArticleFig(id=1320881586237722686, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, label=Fig.8, caption=Sensory scores of tissue morphology, odor and color of B. cereus B1 in pure milk (A, B, C), lactose-hydrolyzed milk (D, E, F) and grain-containing milk (G, H, I), and images of morphological and color changes in three dairy products (J, K, L) at 25 ℃ and 35 ℃, figureFileSmall=lhQolggrcFyYibDjRbJZTQ==, figureFileBig=j/MCHXPcBALP4lQ/5W+Tpg==, tableContent=null), ArticleFig(id=1320881586321608767, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, label=图8, caption=25 ℃和35 ℃下蜡样芽孢杆菌B1在纯牛奶中(A、B、C)、舒化牛奶中(D、E、F)和谷粒牛奶中(G、H、I)的组织形态、气味和色泽的评分以及在三种不同类型奶制品中的形态和色泽实际变化图片(J、K、L), figureFileSmall=lhQolggrcFyYibDjRbJZTQ==, figureFileBig=j/MCHXPcBALP4lQ/5W+Tpg==, tableContent=null), ArticleFig(id=1320881586392911936, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, label=Table 1, caption=

Samples collection information

, figureFileSmall=null, figureFileBig=null, tableContent=
样品编号采样时间样品来源样品名称
1号2022.04.22前处理罐原料乳
2号2022.04.22前处理罐原料乳
3号2022.04.22前处理罐原料乳
4号2022.04.22前处理罐原料乳
5号2022.04.22前处理罐原料乳
6号2022.04.22前处理罐原料乳
7号2022.04.22前处理罐原料乳
8号2022.06.08前处理罐原料乳
9号2022.06.08前处理罐原料乳
10号2022.06.08前处理罐原料乳
11号2022.06.08前处理罐原料乳
12号2022.06.08前处理罐原料乳
13号2022.06.08前处理罐原料乳
14号2022.06.08前处理罐原料乳
15号2022.08.03前处理罐原料乳
16号2022.08.03前处理罐原料乳
17号2022.08.03前处理罐原料乳
18号2022.08.03前处理罐原料乳
19号2022.08.03前处理罐原料乳
20号2022.08.03前处理罐原料乳
21号2022.08.03前处理罐原料乳
22号2022.08.03前处理罐原料乳
23号2022.08.03前处理罐原料乳
24号2022.08.03前处理罐原料乳
25号2022.08.03前处理罐原料乳
26号2022.05.25淘宝电商商品乳
27号2022.05.25淘宝电商商品乳
28号2022.05.25淘宝电商商品乳
29号2022.05.25淘宝电商商品乳
30号2022.05.25淘宝电商商品乳
31号2022.05.25淘宝电商商品乳
32号2022.05.25淘宝电商商品乳
33号2022.05.25淘宝电商商品乳
34号2022.05.25淘宝电商商品乳
35号2022.05.25淘宝电商商品乳
36号2022.05.25永辉超市商品乳
37号2022.05.25永辉超市商品乳
38号2022.05.25永辉超市商品乳
39号2022.05.25永辉超市商品乳
40号2022.05.25永辉超市商品乳
41号2022.05.25永辉超市商品乳
42号2022.05.25永辉超市商品乳
43号2022.05.25永辉超市商品乳
44号2022.05.25华润万家超市商品乳
45号2022.05.25华润万家超市商品乳
46号2022.05.25华润万家超市商品乳
47号2022.05.25华润万家超市商品乳
48号2022.05.25华润万家超市商品乳
49号2022.05.25华润万家超市商品乳
50号2022.05.25华润万家超市商品乳
), ArticleFig(id=1320881586451632193, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, label=表1, caption=

样品采集信息

, figureFileSmall=null, figureFileBig=null, tableContent=
样品编号采样时间样品来源样品名称
1号2022.04.22前处理罐原料乳
2号2022.04.22前处理罐原料乳
3号2022.04.22前处理罐原料乳
4号2022.04.22前处理罐原料乳
5号2022.04.22前处理罐原料乳
6号2022.04.22前处理罐原料乳
7号2022.04.22前处理罐原料乳
8号2022.06.08前处理罐原料乳
9号2022.06.08前处理罐原料乳
10号2022.06.08前处理罐原料乳
11号2022.06.08前处理罐原料乳
12号2022.06.08前处理罐原料乳
13号2022.06.08前处理罐原料乳
14号2022.06.08前处理罐原料乳
15号2022.08.03前处理罐原料乳
16号2022.08.03前处理罐原料乳
17号2022.08.03前处理罐原料乳
18号2022.08.03前处理罐原料乳
19号2022.08.03前处理罐原料乳
20号2022.08.03前处理罐原料乳
21号2022.08.03前处理罐原料乳
22号2022.08.03前处理罐原料乳
23号2022.08.03前处理罐原料乳
24号2022.08.03前处理罐原料乳
25号2022.08.03前处理罐原料乳
26号2022.05.25淘宝电商商品乳
27号2022.05.25淘宝电商商品乳
28号2022.05.25淘宝电商商品乳
29号2022.05.25淘宝电商商品乳
30号2022.05.25淘宝电商商品乳
31号2022.05.25淘宝电商商品乳
32号2022.05.25淘宝电商商品乳
33号2022.05.25淘宝电商商品乳
34号2022.05.25淘宝电商商品乳
35号2022.05.25淘宝电商商品乳
36号2022.05.25永辉超市商品乳
37号2022.05.25永辉超市商品乳
38号2022.05.25永辉超市商品乳
39号2022.05.25永辉超市商品乳
40号2022.05.25永辉超市商品乳
41号2022.05.25永辉超市商品乳
42号2022.05.25永辉超市商品乳
43号2022.05.25永辉超市商品乳
44号2022.05.25华润万家超市商品乳
45号2022.05.25华润万家超市商品乳
46号2022.05.25华润万家超市商品乳
47号2022.05.25华润万家超市商品乳
48号2022.05.25华润万家超市商品乳
49号2022.05.25华润万家超市商品乳
50号2022.05.25华润万家超市商品乳
), ArticleFig(id=1320881586527129666, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, label=Table 2, caption=

Sensory evaluation scores

, figureFileSmall=null, figureFileBig=null, tableContent=
项目评分标准分值
组织状态组织均匀细腻,质地光滑,无分层31~40
质地较细腻,有少量脂肪膜及蛋白质凝结,无气泡16~30
质地较粗糙,有气泡产生,有乳清析出或出现明显分层0~15
气味产品风味和奶香协调,无异常或其他味道21~30
产品风味减弱,有轻微奶香11~20
产品风味及奶香味微弱,有酸味或臭味等异味0~10
色泽色泽均匀,有光泽,呈正常乳白色21~30
色泽较差,颜色稍加深11~20
色泽不均匀,颜色变暗变黄,无光泽0~10
), ArticleFig(id=1320881588209045571, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, label=表2, caption=

感官评价评分

, figureFileSmall=null, figureFileBig=null, tableContent=
项目评分标准分值
组织状态组织均匀细腻,质地光滑,无分层31~40
质地较细腻,有少量脂肪膜及蛋白质凝结,无气泡16~30
质地较粗糙,有气泡产生,有乳清析出或出现明显分层0~15
气味产品风味和奶香协调,无异常或其他味道21~30
产品风味减弱,有轻微奶香11~20
产品风味及奶香味微弱,有酸味或臭味等异味0~10
色泽色泽均匀,有光泽,呈正常乳白色21~30
色泽较差,颜色稍加深11~20
色泽不均匀,颜色变暗变黄,无光泽0~10
), ArticleFig(id=1320881588292931652, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, label=Table 3, caption=

Biochemical identification of three isolates

, figureFileSmall=null, figureFileBig=null, tableContent=
试验代码试验项目名称结果试验代码试验项目名称结果
BXYLβ-木糖苷酶−LeuA亮氨酸芳胺酶+
BGALβ-半乳糖苷酶−AlaA丙氨酸芳胺酶+
APPA丙氨酸-苯丙氨酸-脯氨酸芳胺酶+GLYG糖元+
ELLMELLMAN−MTE麦芽三糖+
dMNED-甘露糖−PLE古老糖−
BMANβ-甘露糖苷酶−AGLUα-葡萄糖苷酶−
INU菊粉−PSCNa腐胺同化−
OLD竹桃霉素耐受−POLYB_R多粘菌素B耐受−
LysAL-赖氨酸芳胺酶−PheA苯丙氨酸芳胺酶+
PyrA吡咯烷基芳胺酶+TyrA酪氨酸芳胺酶+
CDEX环糊精+INO肌醇−
MdX甲基-D-木糖苷−GlyA氨基酸芳胺酶−
PHC磷酰基维生素B−IRHAL-鼠李糖−
dGLUD-葡萄糖+dTAGD-塔格糖−
ESC七叶灵水解+NaCl 6.5%6.5%氯化钠生长+
AspAL-天冬氨酸芳胺酶−ProA脯氨酸芳胺酶−
AGALα-半乳糖苷酶−BNAGN-乙酰-β-D-葡萄糖氨酶+
dGALD-半乳糖−MdG甲基葡萄糖甙酸化−
AMANα-甘露糖苷酶−dMAND-甘露醇−
NAGN-乙酰-D-葡萄糖氨+BGLUβ-葡萄糖苷酶−
PVATE丙酮酸盐+dTRED-海藻糖+
dRIBD-核糖+KAN卡那霉素耐受−
TTZ多粘菌素B耐药−
), ArticleFig(id=1320881588376817733, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, label=表3, caption=

三种分离菌株的生化鉴定结果

, figureFileSmall=null, figureFileBig=null, tableContent=
试验代码试验项目名称结果试验代码试验项目名称结果
BXYLβ-木糖苷酶−LeuA亮氨酸芳胺酶+
BGALβ-半乳糖苷酶−AlaA丙氨酸芳胺酶+
APPA丙氨酸-苯丙氨酸-脯氨酸芳胺酶+GLYG糖元+
ELLMELLMAN−MTE麦芽三糖+
dMNED-甘露糖−PLE古老糖−
BMANβ-甘露糖苷酶−AGLUα-葡萄糖苷酶−
INU菊粉−PSCNa腐胺同化−
OLD竹桃霉素耐受−POLYB_R多粘菌素B耐受−
LysAL-赖氨酸芳胺酶−PheA苯丙氨酸芳胺酶+
PyrA吡咯烷基芳胺酶+TyrA酪氨酸芳胺酶+
CDEX环糊精+INO肌醇−
MdX甲基-D-木糖苷−GlyA氨基酸芳胺酶−
PHC磷酰基维生素B−IRHAL-鼠李糖−
dGLUD-葡萄糖+dTAGD-塔格糖−
ESC七叶灵水解+NaCl 6.5%6.5%氯化钠生长+
AspAL-天冬氨酸芳胺酶−ProA脯氨酸芳胺酶−
AGALα-半乳糖苷酶−BNAGN-乙酰-β-D-葡萄糖氨酶+
dGALD-半乳糖−MdG甲基葡萄糖甙酸化−
AMANα-甘露糖苷酶−dMAND-甘露醇−
NAGN-乙酰-D-葡萄糖氨+BGLUβ-葡萄糖苷酶−
PVATE丙酮酸盐+dTRED-海藻糖+
dRIBD-核糖+KAN卡那霉素耐受−
TTZ多粘菌素B耐药−
), ArticleFig(id=1320881588456509510, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=EN, label=Table 4, caption=

Growth kinetics model equation and fit of B. cereus B1

, figureFileSmall=null, figureFileBig=null, tableContent=
产品类型温度(℃)初始蜡样芽孢杆菌浓度(CFU/mL)生长拟合曲线拟合度
纯牛奶2510~100y=7.1978+(1.1929−7.1978)/[1+(x/13.8132)15.6296]0.9997
100~1000y=7.2336+(2.8102−7.2336)/[1+(x/12.0715)12.1038]0.9994
1000~10000y=7.2256+(3.8167−7.2256)/[1+(x/9.6202)7.5885]0.9993
3510~100y=7.3413+(1.9181−7.3413)/[1+(x/11.4453)8.8364]0.997
100~1000y=7.3105+(2.7023−7.3105)/[1+(x/8.8971)11.3715]0.9989
1000~10000y=7.4009+(3.7702−7.4009)/[1+(x/7.2534)12.1759]0.9993
舒化牛奶2510~100y=7.4012+(1.7672−7.4012)/[1+(x/10.8805)9.0652]0.9897
100~1000y=7.3425+(2.7649−7.3425)/[1+(x/9.0801)9.1756]0.9943
1000~10000y=7.3461+(3.6314−7.3461)/[1+(x/6.5288)7.8917]0.9956
3510~100y=7.3631+(1.6429−7.3631)/[1+(x/8.3205)7.2844]0.9997
100~1000y=7.4256+(2.6167−7.4256)/[1+(x/7.4798)5.3059]0.9865
1000~10000y=7.3201+(3.5480−7.3201)/[1+(x/4.8264)11.5486]0.9934
谷粒牛奶2510~100y=7.3744+(1.5744−7.3744)/[1+(x/16.0868)6.7643]0.9993
100~1000y=7.4122+(2.8088−7.4122)/[1+(x/13.0904)8.8140]0.9997
1000~10000y=7.3800+(3.7948−7.3800)/[1+(x/12.1737)10.4471]0.9944
3510~100y=7.2901+(1.6594−7.2901)/[1+(x/11.6924)5.2307]0.9857
100~1000y=7.3723+(2.5380−7.3723)/[1+(x/9.1851)9.4216]0.9993
1000~10000y=7.3641+(3.7412−7.3641)/[1+(x/7.6309)9.9539]0.9988
), ArticleFig(id=1320881588532006983, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, language=CN, label=表4, caption=

蜡样芽孢杆菌B1生长动力学模型方程及拟合度

, figureFileSmall=null, figureFileBig=null, tableContent=
产品类型温度(℃)初始蜡样芽孢杆菌浓度(CFU/mL)生长拟合曲线拟合度
纯牛奶2510~100y=7.1978+(1.1929−7.1978)/[1+(x/13.8132)15.6296]0.9997
100~1000y=7.2336+(2.8102−7.2336)/[1+(x/12.0715)12.1038]0.9994
1000~10000y=7.2256+(3.8167−7.2256)/[1+(x/9.6202)7.5885]0.9993
3510~100y=7.3413+(1.9181−7.3413)/[1+(x/11.4453)8.8364]0.997
100~1000y=7.3105+(2.7023−7.3105)/[1+(x/8.8971)11.3715]0.9989
1000~10000y=7.4009+(3.7702−7.4009)/[1+(x/7.2534)12.1759]0.9993
舒化牛奶2510~100y=7.4012+(1.7672−7.4012)/[1+(x/10.8805)9.0652]0.9897
100~1000y=7.3425+(2.7649−7.3425)/[1+(x/9.0801)9.1756]0.9943
1000~10000y=7.3461+(3.6314−7.3461)/[1+(x/6.5288)7.8917]0.9956
3510~100y=7.3631+(1.6429−7.3631)/[1+(x/8.3205)7.2844]0.9997
100~1000y=7.4256+(2.6167−7.4256)/[1+(x/7.4798)5.3059]0.9865
1000~10000y=7.3201+(3.5480−7.3201)/[1+(x/4.8264)11.5486]0.9934
谷粒牛奶2510~100y=7.3744+(1.5744−7.3744)/[1+(x/16.0868)6.7643]0.9993
100~1000y=7.4122+(2.8088−7.4122)/[1+(x/13.0904)8.8140]0.9997
1000~10000y=7.3800+(3.7948−7.3800)/[1+(x/12.1737)10.4471]0.9944
3510~100y=7.2901+(1.6594−7.2901)/[1+(x/11.6924)5.2307]0.9857
100~1000y=7.3723+(2.5380−7.3723)/[1+(x/9.1851)9.4216]0.9993
1000~10000y=7.3641+(3.7412−7.3641)/[1+(x/7.6309)9.9539]0.9988
)], attaches=null, journal=Journal(id=1260987317930967064, delFlag=0, nameCn=食品工业科技, nameEn=Science and Technology of Food Industry, nameHistory1=null, nameHistory2=null, issn=1002-0306, eissn=null, cn=11-1759/TS, coden=null, periodic=3, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=pHKBTDK6IiDT2yQZYevC/g==, journalPrice=null, startedYear=null, abbrevIsoEn=Science and Technology of Food Industry, journalRemark=null, publicationField=null, createdTime=1778572404609, updatedTime=1788949639446, createdBy=18614031015, updatedBy=13041195026, firstLetterCn=S, firstLetterEn=S, subjectCode=Agriculture, subjectName=null, subjectCodeEn=Agriculture, subjectNameEn=null, picCn=pHKBTDK6IiDT2yQZYevC/g==, picEn=k9cXIKW0r+dhvmYbf8WDQQ==, jcr=null, cjcr=null, exts=[JournalExt(id=1304512595617145106, language=CN, name=食品工业科技, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788949639467, updatedTime=1788949639467, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://spkj.cbpt.cnki.net/EditorB2N/index.aspx?t=1, submissionEditorUrl=https://spkj.cbpt.cnki.net/EditorB2N/index.aspx?t=3, submissionReviewUrl=https://spkj.cbpt.cnki.net/EditorB2N/index.aspx?t=2, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1304512595688448275, language=EN, name=Science and Technology of Food Industry, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788949639484, updatedTime=1788949639484, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://spkj.cbpt.cnki.net/EditorB2N/index.aspx?t=1, submissionEditorUrl=https://spkj.cbpt.cnki.net/EditorB2N/index.aspx?t=3, submissionReviewUrl=https://spkj.cbpt.cnki.net/EditorB2N/index.aspx?t=2, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1260987677001138203, websiteList=[Website(id=1260990269093196609, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1260987677001138203, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/spgykj/CN, language=CN, createTime=1778573108218, createBy=18614031015, updateTime=1778573937722, updateBy=18614031015, name=食品工业科技-中文, tplId=1146099689490845704, title=食品工业科技, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1260993882188333840, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269093196609, code=articleTextType, value=kx, createTime=1778573969647, updateTime=1778573969647, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993881970230029, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269093196609, code=banner, value=null, createTime=1778573969595, updateTime=1778573969595, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993882259637011, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269093196609, code=grayFlag, value=0, createTime=1778573969664, updateTime=1778573969664, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993881940869900, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269093196609, code=logo, value=https://castjournals.cast.org.cn/joweb/spgykj/CN/file/pic?fileId=m5/V/nQKn3zR0EIDYaiOEA==, createTime=1778573969588, updateTime=1778573969588, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993882284802837, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269093196609, code=minRunFlag, value=0, createTime=1778573969670, updateTime=1778573969670, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993882175750927, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269093196609, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/spgykj/CN/file/pic, createTime=1778573969644, updateTime=1778573969644, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993882276414228, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269093196609, code=silenceFlag, value=0, createTime=1778573969668, updateTime=1778573969668, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993882049921806, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269093196609, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1778573969614, updateTime=1778573969614, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993882196722449, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269093196609, code=themeColor, value=null, createTime=1778573969649, updateTime=1778573969649, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993882234471186, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269093196609, code=themeStyle, value=null, createTime=1778573969658, updateTime=1778573969658, creator=18614031015, updator=18614031015)]), Website(id=1260990269265163062, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1260987677001138203, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/spgykj/EN, language=EN, createTime=1778573108259, createBy=18614031015, updateTime=1778573934408, updateBy=18614031015, name=食品工业科技-英文, tplId=1146101810881728533, title=Science and Technology of Food Industry, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1260993842489246463, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269265163062, code=articleTextType, value=kx, createTime=1778573960182, updateTime=1778573960182, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993842438914812, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269265163062, code=banner, value=null, createTime=1778573960170, updateTime=1778573960170, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993842510217986, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269265163062, code=grayFlag, value=0, createTime=1778573960187, updateTime=1778573960187, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993842430526203, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269265163062, code=logo, value=https://castjournals.cast.org.cn/joweb/spgykj/EN/file/pic?fileId=m5/V/nQKn3zR0EIDYaiOEA==, createTime=1778573960168, updateTime=1778573960168, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993842531189508, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269265163062, code=minRunFlag, value=0, createTime=1778573960192, updateTime=1778573960192, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993842476663550, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269265163062, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/spgykj/EN/file/pic, createTime=1778573960179, updateTime=1778573960179, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993842522800899, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269265163062, code=silenceFlag, value=0, createTime=1778573960190, updateTime=1778573960190, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993842447303421, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269265163062, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1778573960172, updateTime=1778573960172, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993842497635072, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269265163062, code=themeColor, value=null, createTime=1778573960184, updateTime=1778573960184, creator=18614031015, updator=18614031015), WebsiteProps(id=1260993842506023681, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1260990269265163062, code=themeStyle, value=null, createTime=1778573960186, updateTime=1778573960186, creator=18614031015, updator=18614031015)])], journalTitle=食品工业科技, weixinUrl=null, journalUrl=https://www.spgykj.com/, iacademicId=null, status=1, seqNo=null, journalTitleEn=Science and Technology of Food Industry, journalPhotoCn=pHKBTDK6IiDT2yQZYevC/g==, journalPhotoEn=k9cXIKW0r+dhvmYbf8WDQQ==, journalFirstLetter=S, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/spgykj/CN/10.13386/j.issn1002-0306.2025090242, detailUrlEn=https://castjournals.cast.org.cn/joweb/spgykj/EN/10.13386/j.issn1002-0306.2025090242, pdfUrlCn=https://castjournals.cast.org.cn/joweb/spgykj/CN/PDF/10.13386/j.issn1002-0306.2025090242, pdfUrlEn=https://castjournals.cast.org.cn/joweb/spgykj/EN/PDF/10.13386/j.issn1002-0306.2025090242, aliStartDate=0, aliEndDate=0, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=1, orderTime=1788192000000, fullTextJson=null, articleText=null, reference=null)
收藏切换
蜡样芽孢杆菌在三种不同乳产品中的生长动力学特性以及引起乳制品感官变化特征
收藏切换
PDF下载
陈强强 1 , 寇肖迪 1 , 徐海俊 1 , 胡瑜 2 , 唐俊妮 1, *
食品工业科技 | 生物工程 2026,47(17): 233-243
收起
收藏切换
食品工业科技 |生物工程 2026 , 47 (17) : 233 -243
蜡样芽孢杆菌在三种不同乳产品中的生长动力学特性以及引起乳制品感官变化特征
全屏
[Author(id=1320881579589750798, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=2338659742@qq.com, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320881579661053968, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881579589750798, language=EN, stringName=Qiangqiang CHEN, firstName=Qiangqiang, middleName=null, lastName=CHEN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320881579723968529, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881579589750798, language=CN, stringName=陈强强, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1西南民族大学药学与食品学院,四川成都 610225, bio={"content":"

陈强强(1999−),女,硕士研究生,研究方向:食品加工与安全,E-mail:

"}, bioImg=null, bioContent=

陈强强(1999−),女,硕士研究生,研究方向:食品加工与安全,E-mail:

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320881579409395719, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, xref=1, ext=[AuthorCompanyExt(id=1320881579417784328, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China), AuthorCompanyExt(id=1320881579421978633, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1西南民族大学药学与食品学院,四川成都 610225)])]), Author(id=1320881579803660307, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320881579874963477, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881579803660307, language=EN, stringName=Xiaodi KOU, firstName=Xiaodi, middleName=null, lastName=KOU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320881579946266646, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881579803660307, language=CN, stringName=寇肖迪, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1西南民族大学药学与食品学院,四川成都 610225, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320881579409395719, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, xref=1, ext=[AuthorCompanyExt(id=1320881579417784328, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China), AuthorCompanyExt(id=1320881579421978633, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1西南民族大学药学与食品学院,四川成都 610225)])]), Author(id=1320881580004986904, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320881580072095770, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881580004986904, language=EN, stringName=Haijun XU, firstName=Haijun, middleName=null, lastName=XU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320881580151787547, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881580004986904, language=CN, stringName=徐海俊, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1西南民族大学药学与食品学院,四川成都 610225, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320881579409395719, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, xref=1, ext=[AuthorCompanyExt(id=1320881579417784328, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China), AuthorCompanyExt(id=1320881579421978633, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1西南民族大学药学与食品学院,四川成都 610225)])]), Author(id=1320881580340531229, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320881580453777439, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881580340531229, language=EN, stringName=Yu HU, firstName=Yu, middleName=null, lastName=HU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2SIG Combibloc (Suzhou) Co., Ltd., Suzhou 215021, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320881580533469216, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881580340531229, language=CN, stringName=胡瑜, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2康美包(苏州)有限公司,江苏苏州 215021, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320881579493281802, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, xref=2, ext=[AuthorCompanyExt(id=1320881579501670411, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579493281802, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2SIG Combibloc (Suzhou) Co., Ltd., Suzhou 215021, China), AuthorCompanyExt(id=1320881579510059020, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579493281802, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2康美包(苏州)有限公司,江苏苏州 215021)])]), Author(id=1320881580617355298, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=392783291@qq.com, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1320881580688658468, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881580617355298, language=EN, stringName=Junni TANG, firstName=Junni, middleName=null, lastName=TANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, *, address=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320881580764155941, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, authorId=1320881580617355298, language=CN, stringName=唐俊妮, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, *, address=1西南民族大学药学与食品学院,四川成都 610225, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320881579409395719, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, xref=1, ext=[AuthorCompanyExt(id=1320881579417784328, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China), AuthorCompanyExt(id=1320881579421978633, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320881272558306118, companyId=1320881579409395719, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1西南民族大学药学与食品学院,四川成都 610225)])])]
陈强强1 , 寇肖迪1, 徐海俊1, 胡瑜2, 唐俊妮1, *
作者信息
  • 1西南民族大学药学与食品学院,四川成都 610225
  • 2康美包(苏州)有限公司,江苏苏州 215021
通讯作者:
唐俊妮(1971−),女,博士,教授,研究方向:食品微生物与食品安全,E-mail:。
作者简介:

陈强强(1999−),女,硕士研究生,研究方向:食品加工与安全,E-mail:

Growth Kinetics and Organoleptic Characterization of Bacillus cereus in Three Different Dairy Products
Qiangqiang CHEN1 , Xiaodi KOU1, Haijun XU1, Yu HU2, Junni TANG1, *
Affiliations
  • 1College of Pharmacy and Food, Southwest Minzu University, Chengdu 610225, China
  • 2SIG Combibloc (Suzhou) Co., Ltd., Suzhou 215021, China
出版时间: 2026-09-01 doi: 10.13386/j.issn1002-0306.2025090242
文章导航
收藏切换

为探究蜡样芽孢杆菌在不同牛奶产品中的生长动力学特性及对牛奶品质影响,本研究采集了50份乳制品(包括25份工厂前处理罐原料乳和25份市售纸盒包装商品乳),通过甘露醇卵黄多粘菌素琼脂培养基(MYP)分离疑似菌株,结合形态学观察、蛋白质毒素结晶实验、Vitek-2生化鉴定及16S rRNA测序分离鉴定蜡样芽孢杆菌;以筛选生长速率最快的菌株作为实验对象,按101、102、103 CFU/mL初始浓度回接至三种不同牛奶产品(纯牛奶、舒化牛奶和谷粒牛奶)中,研究在两种温度(25 ℃和35 ℃)下,蜡样芽孢杆菌在三种不同产品中的生长动力学,采用Logistic方程拟合生长模型,同步监测三种牛奶产品的pH变化和感官影响。结果显示:分离鉴定的3株蜡样芽孢杆菌(B1、B2和B3)均源自原料乳,市售乳中未检出;通过生长曲线测定,研究B1菌株对三种不同牛奶产品的影响,结果表明温度和初始接种浓度对蜡样芽孢杆菌的生长影响较为明显,在35 ℃条件下和高初始接种浓度下,乳制品中的蜡样芽孢杆菌较25 ℃更早进入对数期和稳定期,迟滞期更短,三种类型的产品中,舒化牛奶更有利于蜡样芽孢杆菌的生长;Logistic模型拟合度R2>0.9857;牛奶的pH随菌浓度增长变化明显,不同乳制品感官劣变具有各自的特异性,其中舒化牛奶气味劣变最为显著。本研究为乳制品蜡样芽孢杆菌污染预警及安全控制提供科学依据

蜡样芽孢杆菌  /  乳品  /  生长动力学  /  pH变化  /  感官品质

The purpose of this study was to investigate the growth kinetics of Bacillus cereus in different milk products and its impact on milk quality. Fifty dairy products (including 25 raw milk from factory pre-treatment tanks and 25 commercial milk packaged in boxes) were collected, and suspected strains were isolated using Mannitol-Egg-Yolk-Polymyxin Agar (MYP). Morphological observation, protein toxin crystallization experiment, Vitek-2 biochemical identification, and 16S rRNA sequencing were used to isolate and identify B. cereus. Further, one strain with the fastest growth rate was selected as the experimental object, and the strain was inoculated into three different milk products (pure milk, lactose-decomposed milk, and grain milk) at initial concentrations of 101, 102, and 103 CFU/mL. The growth kinetics of B. cereus in three different products were studied at two temperatures (25 ℃ and 35 ℃), and a Logistic equation was used to fit the growth model. The pH and sensory changes of the three milk products were simultaneously monitored. The results showed that the three strains (B1, B2, and B3) of B. cereus originated from raw milk were isolated and identified and they were not detected in commercial milk products. By measuring the growth curve, B1 strain with a faster growth rate was selected as the experimental object to study its effects on three different milk products. The results showed that temperature and initial inoculation concentration had a more significant impact on the growth of B. cereus. At 35 ℃ and high initial inoculation concentration, B. cereus in dairy products entered the logarithmic and stable phases earlier than at 25 ℃, with a shorter lag period. Among the three types of products, lactose-decomposed milk was more conducive to the growth of B. cereus. The fitting degree of the Logistic model was R2>0.9857. It was found that the pH of milk changed significantly as the bacterial concentration increased. Different dairy products had their own characteristics in terms of sensory degradation. Among them, the odor deterioration of lactose-decomposed milk was the most significant. This study provides a scientific basis for the early warning and safety control of B. cereus contamination in dairy products.

Bacillus cereus  /  dairy products  /  growth kinetics  /  pH changes  /  sensory quality
陈强强, 寇肖迪, 徐海俊, 胡瑜, 唐俊妮. 蜡样芽孢杆菌在三种不同乳产品中的生长动力学特性以及引起乳制品感官变化特征. 食品工业科技, 2026 , 47 (17) : 233 -243 . DOI: 10.13386/j.issn1002-0306.2025090242
Qiangqiang CHEN, Xiaodi KOU, Haijun XU, Yu HU, Junni TANG. Growth Kinetics and Organoleptic Characterization of Bacillus cereus in Three Different Dairy Products[J]. Science and Technology of Food Industry, 2026 , 47 (17) : 233 -243 . DOI: 10.13386/j.issn1002-0306.2025090242
随着全球乳制品消费量的持续增长及消费者对营养健康需求的不断提升,乳制品质量安全已成为食品工业和公共卫生关切的核心。据OECD-FAO预测,2029年全球牛奶产量将达到9.97亿吨[1],中国作为全球最大的乳制品新兴市场,人均乳制品消费量逐年攀升[2]。然而,乳及乳制品富含优质蛋白、乳糖和脂肪,为微生物繁殖提供了理想基质,其从牧场到餐桌的复杂供应链极易遭受致病菌污染,引发腐败变质与食源性疾病[3]。近年来,由微生物污染导致的乳制品安全事件频发,不仅造成巨大经济损失,更严重威胁消费者健康,其中蜡样芽孢杆菌已成为全球乳制品召回的主要原因之一[4]。
蜡样芽孢杆菌是一种广泛分布于土壤、尘埃、水体及食品生产环境的革兰氏阳性产芽孢食源性致病菌[5]。其芽孢具有极强的耐热性,可耐受传统巴氏杀菌(72~85 ℃)和超高温瞬时灭菌(UHT),在成品乳中长期存活,于适宜条件下萌发并快速增殖[6]。该菌在乳制品中的污染率高达10%~40%,其致病因子主要包括致腹泻肠毒素和致呕吐毒素,摄入105~108 CFU/mL的活菌或1.5~12 μg/kg的呕吐毒素即可引发中毒症状[7]。蜡样芽孢杆菌可在4~8 ℃冷藏条件下缓慢生长,对低温长货架期乳制品构成持续威胁[8]。据估计,全球约1.4%~12%食源性疾病由蜡样芽孢杆菌引起[9]。
蜡样芽孢杆菌在乳制品中的生长动力学是预测其安全风险和货架期的核心参数,最大比生长速率等关键动力学参数决定产品不同贮藏条件下的安全时限,细菌生长受原料源头、温度、基质组成、初始菌浓度等多因子影响。Tirloni等[3]对意大利乳制品生产链的长期监测表明,该菌在原料乳中检出率为10.3%~35%,主要污染源为挤奶设备和青贮饲料,巴氏杀菌后成品乳中检出率显著降低。郭佳等[4]发现中国北方牧区原料乳污染率较高,粗饲料储存不当成为主要的风险因子。Zhao等[10]在全国10省份的调研发现夏季样品蜡样芽孢杆菌污染率(28.7%)显著高于冬季(8.5%)样品。Le Marc等[8]通过Stochastic模型证实,蜡样芽孢杆菌最适生长温度为30~37 ℃。而Maktabdar等[11]发现冷藏条件下菌株仍可缓慢生长,对低温长货架期乳制品构成威胁。基质组成也会显著影响生长,乳制品中乳糖、蛋白质及添加成分可改变细菌的营养利用效率[12−14];谷物中的膳食纤维可通过竞争营养延缓菌株增殖[12−13];此外,乳蛋白中的κ-酪蛋白可被蜡样芽孢杆菌蛋白酶水解,不仅为菌株提供氮源,还可改变乳体系稳定性,间接影响生长环境[13,15]。王慧莹[16]指出高碳水化合物和蛋白质含量可促进蜡样芽孢杆菌增殖。舒化奶乳糖分解或加速生长,谷粒奶添加成分可影响代谢[12,17]。Yang等[17]在对再制乳生产链的暴露评估中证实产品配方差异会显著改变菌体增殖速率。目前仍缺乏针对舒化奶、谷物奶等创新液态乳中蜡样芽孢杆菌生长的研究。Logistic模型因参数简洁、拟合度高,常被用于描述微生物“S”型生长过程[18]。但将其与乳品pH变化、感官劣变结合的多指标联合预警模型尚未见报道。蜡样芽孢杆菌导致乳制品感官劣变是其危害的重要表现形式,该菌分泌的热稳定蛋白酶与脂肪酶是主要致腐因子,蛋白酶特异性水解κ-酪蛋白,破坏酪蛋白胶束稳定性,导致蛋白质凝结、乳清析出及苦味肽产生[19];脂肪酶释放的中短链游离脂肪酸是酸败味的主要来源[12];在谷粒牛奶中,蜡样芽孢杆菌产生的淀粉酶可降解燕麦多糖,产生短链脂肪酸影响谷物原有风味[20]。基于上述研究现状,本研究以纯牛奶、舒化牛奶、谷粒牛奶三种典型乳制品为对象,探究蜡样芽孢杆菌在不同温度(25、35 ℃)和不同初始污染水平(101~103 CFU/mL)下的生长动力学规律及乳基质类型对菌株生长的交互影响,构建适用于三种乳制品的Logistic生长预测模型、同步监测菌株生长过程中乳制品pH变化与感官劣变特征,建立菌浓度-pH-感官评分的关联关系。旨在为乳制品蜡样芽孢杆菌污染的源头管控、生长风险预测及品质劣变预警提供科学依据,助力乳制品工业的安全高效发展。
实验所用牛奶样品系无菌采集于某工厂前处理罐中牛奶样品共25份,以及采集自电商和超市售卖的纸盒包装纯牛奶样品共25份(详见表1);实验菌株 采用从样品中分离并鉴定的菌株;蜡样芽孢杆菌(CICC21261)、苏云金芽孢杆菌(CICC22945)、蕈状芽孢杆菌(CICC21473) 三株菌株均购买于中国工业微生物菌种保藏管理中心;甘露醇卵黄多粘菌素琼脂培养基(MYP)、胰酪大豆胨琼脂培养基 广东环凯微生物科技有限公司;营养琼脂培养基、硫酸锰营养琼脂培养基 青岛海博生物技术有限公司;平板计数琼脂培养基、林格试液片剂 德国默克生命科学技术有限公司;Vitek-2鉴定所用试剂及配套测试卡片 生物梅里埃美国股份有限公司。
DW-HL550低温冰箱 中科美菱低温科技股份有限公司;Vitek 2 System全自动微生物鉴定仪 生物梅里埃美国股份有限公司;SN510C高压蒸汽灭菌锅 重庆雅马拓科技有限公司;1120 BBC 86生物安全柜 山东博科生物产业有限公司;DHP-9272电热恒温培养箱 上海一恒科学仪器有限公司;Ecotron振荡培养箱、TSGP10水浴锅 赛默飞世尔科技有限公司;H1650K台式微量高速离心机 湘仪仪器有限公司;MS 3B S25涡旋混匀仪 德国IKA公司;Bax Q7荧光实时定量PCR仪 美国Hygiena公司;BSH-2细胞破碎仪 杭州遂真生物技术有限公司;DRY BATH恒温干浴器 日本ASONE公司;移液器 德国Eppendorf公司;S600双目生物显微镜 德国LIOO公司。
样品采集如表1所示,在采集不同批次的前处理牛奶样品时,使用移液器和一次性无菌移液管,每次各准确移取15 mL样品至灭菌空试管内,将样品按照采集时间顺序按照1号至25号依次进行编号,随后置于4 ℃环境冷藏保存备用。对于成品盒装牛奶,采集前先用75%酒精对纸盒吸管口进行消毒,随后利用移液器和一次性无菌移液管,从每盒牛奶产品中精准采集15 mL乳样,转移至灭菌空试管,按采集时间顺序从26号至50号依次编号,并同样放置在4 ℃环境中冷藏保存,以待后续使用。参考郭佳等[4]方法,使用无菌接种环取试管内样品划线接种于MYP平板上,30 ℃条件下培养24 h;挑取分离得到的疑似菌株划线接种于营养琼脂培养基上,37 ℃条件下培养24 h;挑取单菌落再次划线接种于营养琼脂培养基,在37 ℃条件下培养24 h进行菌株纯化。
镜检:挑取营养琼脂平板上的纯培养物适量涂布于滴有无菌生理盐水的载玻片上,干燥后固定,滴加1~2滴草酸铵结晶紫染色1 min后水洗至无色,滴加适量碘液染色1 min后水洗至无色,滴加95%乙醇脱色约30 s后水洗,滴加1~2滴沙黄染液复染1 min后水洗并干燥,使用显微镜进行镜检。
生化鉴定:使用Vitek-2方法鉴定细菌,参考黄立波[21]的方法并稍作调整,取3 mL生理盐水于试管,用无菌拭子蘸取营养琼脂平板上的纯培养物,制备三株分离菌株和蜡样芽孢杆菌(CICC21261)的菌悬液,调整麦氏浊度至0.5 McF,将菌悬液放入载卡架槽,扫描条形码输入样品编号,载卡架放入装载仓,填空卡片后转移至孵育仓,仪器自动检测并显示结果。
根状生长实验:参照国标[22]和文献[23],挑取可疑菌落,间隔2~3 cm划平行直线于干燥1~2 d的营养琼脂平板,30±1 ℃培养24~48 h,以蕈状芽孢杆菌(CICC21473)作为对照。
蛋白质毒素结晶实验:参照国标[22],挑取可疑菌落接种于硫酸锰营养琼脂平板,30±1 ℃培养24±2 h,室温放置3~4 d;取培养物于载玻片,加蒸馏水涂膜,自然干燥后微火固定,加甲醇作用30 s,火焰干燥,滴0.5%碱性复红,加热1~2 min,更换染色液再加热30 s,清洗晾干后镜检;观察游离芽孢(浅红色)和深红色菱形蛋白结晶体;若游离芽孢不丰富,室温再培养2~3 d后检查,以苏云金芽孢杆菌(CICC22945)作为对照。
将分离筛选的菌株命名为B1、B2、B3,挑取纯化后的单个菌落打散于林格氏液中,涡流混匀(2500 r/min,30 s),取1 mL菌液离心(4 ℃,12000 r/min,2 min),弃上清,收集菌体用于DNA提取。采用蛋白酶K裂解法[24],加入50 μL蛋白酶K裂解液混匀,55 ℃水浴1 h,12000 r/min离心2 min,取上清液于95~97 ℃加热10 min保存备用。
PCR反应体系:BioLinker 2×Taq Mix 20 μL,使用16S rRNA基因通用引物27F(5′-AGAGTTTGATCCTGGCTCAG-3′)和1492R(5′-GGTTACCTTGTTACGACTT-3′)进行PCR扩增与测序鉴定[25],DNA模板1~3 μL,dd H2O补至40 μL。
扩增条件:94 ℃预变性3 min;94 ℃变性30 s,56 ℃退火30 s,72 ℃延伸90 s,循环30次。PCR产物经4%琼脂糖凝胶电泳,回收约1500 bp单一条带送到生工生物工程(上海)股份有限公司进行测序。
将灭菌融化的胰酪大豆胨琼脂培养基置于45 ℃水浴锅中保温备用,随后取经传代培养并完成活菌计数的菌悬液,用磷酸盐缓冲液(PBS)梯度稀释至3×104、3×103、3×102 CFU/mL浓度梯度。各取1 mL稀释菌液分别接种至9 mL不同牛奶产品(纯牛奶、舒化牛奶、谷粒牛奶)中,制备污染浓度为3×103、3×102、3×101 CFU/mL的样品,使用涡流混匀仪以2500 r/min转速振荡混匀30 s,确保菌液与样品充分结合。所有样品按培养条件(25 ℃或35 ℃)、基质类型及初始菌浓度进行系统编号:纯牛奶组中,25 ℃培养的101、102、103 CFU/mL样品分别标记为25-C-1、25-C-2、25-C-3,35 ℃培养的对应样品标记为35-C-1、35-C-2、35-C-3;舒化牛奶组和谷粒牛奶组依次类推,分别标记为:25-S-1、25-S-2、25-S-3;35-S-1、35-S-2、35-S-3;25-G-1、25-G-2、25-G-3;35-G-1、35-G-2、35-G-3。完成编号后,所有样品分别转移至25 ℃和35 ℃恒温培养箱中进行培养。
对分离鉴定出的三株蜡样芽孢杆菌(B1、B2和B3)进行回接筛选,以用于后续实验。在25 ℃下,以纯牛奶为培养基质,三株菌株起始浓度均为3×102 CFU/mL,分别在培养的第0~72 h取样,使用涡流混匀仪2500 r/min转速下30 s混匀,使用PBS进行适宜梯度的稀释后取1 mL稀释液于无菌培养皿内,随后倾倒约15 mL 45 ℃左右的平板计数琼脂培养基,每个样品3个平行,立即平移晃动摇匀培养皿中的混合物,待凝固后置于37 ℃培养18~24 h(使用甘露醇卵黄多粘菌素琼脂培养基鉴定是否有杂菌污染)。观察并统计适宜稀释度平板中菌落数量,计算每个时间段每份样品中的细菌数并绘制生长曲线,选取生长速率快的一株菌株作为后续研究对象,进一步研究其在三种不同乳制品中的生长动力学。
采用1.2.5中筛选的菌株进行实验,样品制备详见1.2.4,开展两种温度(25 ℃和35 ℃)和三种类型牛奶(纯牛奶、舒化牛奶、谷粒牛奶)中蜡样芽孢杆菌的生长曲线测定。在实验进程的第0~72 h取样测定细菌总数,每个样品设置3个平行。使用Logistic方程[26−28]模拟蜡样芽孢杆菌在不同温度和不同奶制品中的生长动力学模型。公式如下:
$\rm \text{N}(\text{t})=\frac{{\text{N}}_{\max }}{\left\{1+\exp \left[\dfrac{4{\text{μ}}_{\max }}{{\text{N}}_{\max }({\lambda-t})}+2\right]\right\}} $
式中,t代表培养时间(h),Nmax为蜡样芽孢杆菌的最大生长量(CFU/mL),μmax为蜡样芽孢杆菌的最大生长速率(h-1),λ为迟滞期时长(h)。
按照1.2.4的制备方法,分别在0~72 h内定时取样,涡旋混匀30 s后,用pH计测定各组样品pH。
灭菌干燥西林瓶,取三种产品各9 mL,加入1 mL 3×(104、103、102) CFU/mL菌液,制备3×(103、102、101) CFU/mL污染样品,密封后按1.2.4进行编号,25、35 ℃避光保存。参考国标[29]及邵景海等[30]方法,对污染产品进行一周内组织状态、气味、色泽的单盲法感官评价。评价小组由20名22~45岁食品相关专业人员组成,男女比例各一半,分析不同产品受污染后的感官变化差异。感官评价评分标准见表2。
用Excel 2020软件对数据进行统计和计算,利用Origin 2021对各项指标数据分析结果进行可视化制图。
经MYP培养基形态特征初步鉴别,在采集自前处理罐的25份原料乳样品中的第7号、12号和16号样品内发现有三株疑似蜡样芽孢杆菌,在25份市售纸盒包装商品乳样品内未发现蜡样芽孢杆菌疑似菌株。细菌在MYP培养基上的形态特征和镜检结果如图1所示,分离的三株菌株革兰染色均为阳性,细胞呈杆状,芽孢位于菌体中心或中心附近偏向一端,芽孢直径与菌体横径相近。
经Vitek-2系统鉴定,由表3可知,三株分离菌株和蜡样芽孢杆菌(CICC21261)均呈现以下生化特征:亮氨酸芳胺酶、苯丙氨酸芳胺酶、丙氨酸-苯丙氨酸-脯氨酸芳胺酶、丙氨酸芳胺酶、吡咯烷基芳胺酶、酪氨酸芳胺酶呈阳性;能水解七叶灵;可利用环糊精、D-葡萄糖、N-乙酰-D-葡萄糖氨、丙酮酸盐、D-核糖、糖元、麦芽三糖、N-乙酰-β-D-葡萄糖氨酶和D-海藻糖作为碳源;并能在6.5%氯化钠高盐环境中生长。符合蜡样芽孢杆菌的生理生化特征。
根状生长试验如图2所示,三株菌均形成典型粗糙山谷状菌落结构。
由图3可知蛋白质毒素结晶实验检测发现菌体可形成浅红色孢子,但未观察到深红色菱形毒素晶体。
综合生化特征分析、形态学观察、根状生长实验和蛋白质毒素结晶实验结果,三株分离菌株初步确定为蜡样芽孢杆菌。
进一步通过16S rRNA测序及系统发育树分析,如图4所示,通过MEGA 64软件对三株菌株构建系统发育树,菌株B1、B2、B3在同一分支上,为蜡样芽孢杆菌的近缘菌株,证实三株分离菌株为蜡样芽孢杆菌。
三株分离菌株B1、B2和B3在纯牛奶中的生长曲线如图5所示,可以看出在25 ℃条件下,以纯牛奶为培养基质,B1菌株的生长速率相对较快,先到达对数期及稳定期,后期选用B1菌株进行回接实验。
市售常温商品乳的日常储存温度在25 ℃左右,在夏季高温天气会达到35 ℃及以上,且由于原料乳、商品包材以及灌装管路等残留蜡样芽孢杆菌,经由灭菌程序后商品乳内蜡样芽孢杆菌含量多为低剂量存在,因而实验选取25 ℃及35 ℃作为实验温度、蜡样芽孢杆菌浓度为103、102、101 CFU/mL作为初始污染浓度,在最大程度上模拟蜡样芽孢杆菌污染的实际情况进行。
蜡样芽孢杆菌B1在纯牛奶中的生长曲线如图6 A所示:25 ℃下,103、102、101 CFU/mL菌液分别于9、11、13 h进入对数期,13、18、20 h达稳定期;35 ℃下则分别于8、9、11 h和12、14、18 h进入相应阶段。对比表明,25 ℃迟滞期均长于35 ℃,35 ℃温度下,对数期和稳定期的到来时间分别比25 ℃提前约4 h和6 h。
蜡样芽孢杆菌B1在舒化牛奶中的生长曲线如图6 B所示:25 ℃下,103、102、101 CFU/mL菌液分别于8、9、10 h进入对数期,18、14、16 h达稳定期;35 ℃下则分别于6、8、9 h和8、10、12 h进入相应阶段。对比表明,25 ℃迟滞期更长,35 ℃对数期和稳定期分别较25 ℃提前约3 h和5 h。
在谷粒牛奶中,蜡样芽孢杆菌B1的生长曲线如图6 C所示:25 ℃下,各浓度菌液进入对数期的时间为10~20 h,20~30 h逐步转入稳定期;而在35 ℃下,时间则提前至第8~10 h和第11~22 h。对比表明,35 ℃条件下的迟滞期、对数生长期均短于25 ℃,且稳定期到来时间平均提前约8 h。
可见,三种不同类型的牛奶中,舒化牛奶更有利于蜡样芽孢杆菌B1的生长,在25 ℃和35 ℃下蜡样芽孢杆菌B1在舒化牛奶中生长要比其他两种类型的牛奶先到达对数生长期。
使用Logistic函数模型建立三种乳产品中蜡样芽孢杆菌的生长动力学模型,详见表4,通过生长动力学方程,可以很好地预测蜡样芽孢杆菌在三种不同乳制品中的生长情况。在不同温度下蜡样芽孢杆菌生长延滞期不同,温度越高,蜡样芽孢杆菌生长延滞期越短,25 ℃时,纯牛奶、舒化牛奶、谷粒牛奶的不同初始接菌浓度的生长曲线中的延滞期均比35 ℃长,在相同温度下,蜡样芽孢杆菌的初始接菌浓度越高,体系中的蜡样芽孢杆菌数量增长越快,能够快速进入对数期并达到稳定期。纯牛奶、舒化牛奶和谷粒牛奶在相同温度下,蜡样芽孢杆菌的生长曲线趋势相似,但生长速率不同,最先达到稳定期的是舒化牛奶,其次是谷粒牛奶和纯牛奶,预测模型的R2˃0.98,表明所建立的生长动力学模型有效和可靠。
pH是监测乳制品变质的重要指标。蜡样芽孢杆菌污染乳制品,产品的pH变化受温度与初始接菌浓度双重影响。温度升高缩短延滞期,加速菌群对数期增殖及产酸代谢,高接菌浓度(103 CFU/mL)直接提升酸化效率。由图7A所示,纯牛奶在25 ℃时pH呈三阶段下降(10 h内pH6.7→pH6.55,10~24 h内→pH6.35,24~72 h内稳定在pH6.2~6.3),35 ℃时10 h内即达pH6.35;从图7B可看出,舒化牛奶的酸化更明显(25 ℃时,72 h至pH4.9,35 ℃,24 h至pH4.8);图7C显示了谷粒牛奶在25 ℃下,24 h分至pH5.5左右,35 ℃下24 h降至pH5.1左右。高温菌体代谢活性增强,促进酸性代谢物积累,细菌进入稳定期后因底物耗尽使pH趋稳,高接菌浓度组到达最低pH的时间可提前12 h。
可见,不同牛奶产品的pH随着蜡样芽孢杆菌浓度升高而降低。蜡样芽孢杆菌处于迟滞期及对数期初期时,其浓度较低,pH降低速率较缓慢;进入对数期中期至稳定期后由于菌的浓度较高,pH降低速率加快,代谢基本完成后pH变化趋于稳定。
纯牛奶受蜡样芽孢杆菌B1污染后的感官变化如图8(A、B、C和J)所示。25 ℃时,101 CFU/mL组第2 d出现组织劣化及异味,第5 d结块、第6 d异味加重,7 d内色泽乳白转乳黄;102~103 CFU/mL组次日即现组织状态、异味和色泽同步劣化且持续加剧;在35 ℃时,浓度为101 CFU/mL组第2 d出现异味,第4 d组织状态明显变化(脂肪上浮、蛋白质凝结),色泽变黄;浓度为102 CFU/mL 组第3 d组织状态改变、产生轻微异味,第4 d后色泽劣变加剧;浓度为103 CFU/mL 组第1 d出现明显异味,第2 d轻微蛋白质凝结,5~7 d内色泽变黄且有乳清析出,导致组织状态评分大幅下降。
舒化牛奶的感官变化如图8(D、E、F和K)所示。25 ℃时,101 CFU/mL组在2 d内出现酸味,后期发展为酸臭,伴有组织状态持续劣变和色泽微黄;102~103 CFU/mL组则在24 h内即出现酸味并持续劣变。35 ℃时,所有浓度组(101~103 CFU/mL)均在第1 d出现酸味,并在5 d内出现组织状态、气味和色泽的同步且显著的感官评分下降,5~7 d出现明显分层。
谷粒牛奶的感官变化如图8(G、H、I和L)所示。25 ℃时,所有组别在24 h内燕麦香气均减弱;101与102 CFU/mL组分别在第2、3 d出现异味和组织状态劣变;103 CFU/mL组则在24 h内即出现异味。35 ℃时,各浓度组在第2 d后均出现组织渣滓化与色泽持续变暗;101 CFU/mL组于第3 d出现明显异味,而102与103 CFU/mL组在第2 d出现酸味,并于第3 d发展为明显酸臭味,4~5 d内色泽明显变暗黄。
可见,蜡样芽孢杆菌B1的污染会显著加速三类乳制品的感官劣变,其影响程度与产品类型、储存温度和初始菌浓密切相关,温度越高、初始染菌浓度越大,感官劣变发生速度越快且程度越严重。三种产品中,舒化奶的感官品质下降最快、最严重,可能归因于牛奶中预分解的乳糖为细菌提供了快速增殖的基质,进而大量分泌蛋白酶和脂肪酶,这些酶类分别导致蛋白质凝结、产生苦味,以及脂肪上浮、产生不良风味。
本研究在原料乳中检出了三株蜡样芽孢杆菌(检出率12.0%),市售商品乳中未检出,明确了原料乳是蜡样芽孢杆菌主要污染源。该检出率虽然低于乔霖楠等[31]的乳制品蜡样芽孢杆菌总体污染率(约为25.50%),但吻合Tirloni等[3]报告的意大利原料乳中蜡样芽孢杆菌检出率范围为10.3%~35.0%。生长动力学结果表明,温度与初始浓度是关键控制因子。35 ℃较25 ℃使迟滞期缩短20%~33%,最大比生长速率提升约40%。在舒化牛奶中,103 CFU/mL组在35 ℃下6 h即进入对数期,较25 ℃(8 h)提前25%。稳定期到达时间从18 h缩短至8 h(降幅55%),该结果与Le Marc等[8]报道的最适生长温度(30~37 ℃)下代谢速率提升结论一致。初始浓度效应呈剂量依赖性,103 CFU/mL组进入稳定期较101 CFU/mL组平均提前4.8 h(纯牛奶)、5.2 h(舒化奶)和6.5 h(谷粒奶)。证实了初始细胞数与迟滞期负相关的理论模型[32]。产品基质也影响蜡样芽孢杆菌生长潜力,舒化牛奶因乳糖预水解,碳源利用效率提升,最大菌量(Nmax)达7.4 log CFU/mL,高于纯牛奶与谷粒牛奶。Logistic模型拟合度R2>0.9857,证实其可精准预测三类产品中菌体生长轨迹。
蜡样芽孢杆菌的生长直接导致产品品质劣变。其生长代谢引发的pH下降与乳品的感官劣变进程同步,且受温度与初始接菌浓度的共同影响。在纯牛奶中,25 ℃、初始接菌量为103 CFU/mL条件下,24 h内pH从6.7降至6.37,而35 ℃时24 h pH下降到6.28,感官劣变表现为异味、结块与色泽变黄。相比之下,舒化牛奶因乳糖被预先水解为葡萄糖和半乳糖,单糖更易于被蜡样芽孢杆菌发酵利用,因而酸化更为迅速和彻底,在25 ℃、103 CFU/mL条件下,24 h内pH从6.53大幅降至5.35,并在72 h降至pH4.9,35℃时更是在24 h内pH降至4.85,其酸化效率显著高于纯牛奶,与Kyritsi等[33]关于碳水化合物组成直接影响微生物产酸速率的结论相符,较低的pH环境加剧了蛋白质胶体稳定性丧失,感官劣变出现早,常伴有明显酸味与分层。对于谷粒牛奶,因含有燕麦淀粉等大分子碳源,需经细菌分泌的胞外酶降解后方可利用,故其酸化速率介于纯牛奶与舒化牛奶之间:在相同25 ℃、103 CFU/mL条件下,24 h内pH从6.61降至5.2,35 ℃下24 h降至pH5.05,这体现了底物可利用度对于细菌代谢速率的影响[34]。谷粒牛奶感官劣变则表现为组织渣滓化、色泽变暗及酸臭味,表明燕麦颗粒可能通过提供营养和影响菌-基质互作加速局部酸化。总体而言,较高的储存温度与初始污染浓度会显著加速感官劣变进程,从而大幅缩短产品货架期。
本研究存在的局限在于只采用了单一菌株,未覆盖蜡样芽孢杆菌群内遗传多样性;只采用了两种恒定的温度,未模拟供应链上温度波动,未来需进一步构建动态温度模型;感官评价缺乏客观仪器验证,未来的研究进一步引入电子鼻与电子舌技术[16]。
本研究以纯牛奶、舒化牛奶、谷粒牛奶为研究对象,系统探究了蜡样芽孢杆菌的污染源头、生长动力学特性及对乳制品品质的影响,得出以下主要结论:a.蜡样芽孢杆菌的污染源头主要为原料乳,市售商品乳经杀菌处理后未检出该菌,多方法联合鉴定筛选出三株蜡样芽孢杆菌;b.温度和初始接种浓度显著影响蜡样芽孢杆菌生长,35 ℃、高初始接种浓度(103 CFU/mL)可缩短迟滞期、加速生长,三种类型的牛奶中,舒化牛奶最适宜蜡样芽孢杆菌生长;c.Logistic模型可精准拟合不同条件下蜡样芽孢杆菌的生长过程,可用于预测菌量动态变化;d.乳制品pH随菌浓度增长呈阶段性下降,感官劣变具有基质特异性。
本研究的学术贡献在于构建了三种基质、两种温度条件下蜡样芽孢杆菌生长预测模型,揭示了乳品基质对菌株生长影响以及不同乳品品质劣变和pH变化之间的关联。研究结果为乳制品蜡样芽孢杆菌污染的源头控制、生长预测及品质劣变预警提供科学依据。未来研究方向将聚焦于:a.补充低温条件下的生长动力学数据,构建全温度范围的生长预测模型;b.开展毒力因子检测,建立“生长特性-毒力水平”的耦合预警体系;c.结合仪器分析技术量化感官劣变指标,提升评价客观性;d.模拟工业动态生产环境,探究多因子交互作用下的污染风险,进一步提升研究结果的实践转化价值。

参考文献 引证文献
排序方式:
1
OECD-FAO agricultural outlook 2022-2031[M]. Paris: OECD Publishing, 2022.
2
中国乳制品工业协会. 2023中国乳制品行业质量安全报告[R]. 北京: 中国乳制品工业协会, 2024. [China Dairy Industry Association. 2023 China dairy industry quality and safety report[R]. Beijing: China Dairy Industry Association, 2024.]
China Dairy Industry Association. 2023 China dairy industry quality and safety report[R]. Beijing: China Dairy Industry Association, 2024.
3
TIRLONI E, STELLA S, CELANDRONI F, et al. Bacillus cereus in dairy products and production plants[J]. Foods, 2022, 11(17): 1−12.
4
郭佳, 王娉, 周继福, 等. 乳源蜡样芽孢杆菌耐药性、毒力因子检测及分子特征研究[J]. 中国农业科技导报, 2021, 23(11): 131−138. [GUO J, WANG P, ZHOU J F, et al. Study on drug resistance and molecular typing of Bacillus cereus from dairy products[J]. China Agricultural Science and Technology Herald, 2021, 23(11): 131−138.]
GUO J, WANG P, ZHOU J F, et al. Study on drug resistance and molecular typing of Bacillus cereus from dairy products[J]. China Agricultural Science and Technology Herald, 2021, 23(11): 131−138.
5
LOGAN N A V P D. Bergey's manual of systematics of archaea and bacteria[M]. Hoboken, New Jersey: John Wiley & Sons, 2015: 1-163.
6
CEUPPENS S, BOON N, UYTTENDAELE M. Diversity of Bacillus cereus group strains is reflected in their broad range of pathogenicity and diverse ecological lifestyles[J]. Fems Microbiology Ecology, 2013, 84(3): 433−450.
7
AUTHORITY E E F S. Scientific opinion on the public health risks of bacterial toxins in dairy products[J]. EFSA Journal, 2016, 14(1): 4362.
8
LE MARC Y, BUSS DA SILVA N, POSTOLLEC F, et al. A stochastic approach for modelling the effects of temperature on the growth rate of Bacillus cereus sensu lato[J]. International Journal of Food Microbiology, 2021, 349: 109241.
9
郑跃, 赵鹏, 谢翔燕, 等. 国内外食品中蜡样芽孢杆菌污染情况及控制研究[J]. 食品与发酵工业, 2026, 52(9): 402−410. [ZHENG Yue, ZHAO Peng, XIE Xiangyan, et al. Progress of the contamination and control of Bacillus cereus in foods at home andabroad[J]. Food and Fermentation Industries, 2026, 52(9): 402−410.]
ZHENG Yue, ZHAO Peng, XIE Xiangyan, et al. Progress of the contamination and control of Bacillus cereus in foods at home andabroad[J]. Food and Fermentation Industries, 2026, 52(9): 402−410.
10
ZHAO S, CHEN J, FEI P, et al. Prevalence, molecular characterization, and antibiotic susceptibility of Bacillus cereus isolated from dairy products in China[J]. Journal of Dairy Science, 2020, 103(5): 3994−4001.
11
MAKTABDAR M, HOUMANN R H, SCHEEL N H, et al. Evaluation and validation of extensive growth and growth boundary models for mesophilic and psychrotolerant Bacillus cereus in dairy products (Part 2)[J]. Frontiers in Microbiology, 2025, 16: 1553903.
12
MEHTA D S, METZGER L E, HASSAN A N, et al. The ability of spore formers to degrade milk proteins, fat, phospholipids, common stabilizers, and exopolysaccharides[J]. Journal of Dairy Science, 2019, 102(12): 10799−10813.
13
CARRASCOSA C, RAHEEM D, RAMOS F, et al. Microbial biofilms in the food industry-A comprehensive review[J]. International Journal of Environmental Research and Public Health, 2021, 18(4): 2014.
14
仪淑敏, 张培正, 李远钊, 等. 蜡样芽孢杆菌在营养肉汤中的生长模型[J]. 食品研究与开发, 2009, 30(9): 69−72. [YI S M, ZHANG P Z, LI Y Z, et al. Growth of Bacillus cereus in nutritional broth[J]. Food Research and Development, 2009, 30(9): 69−72.]
YI S M, ZHANG P Z, LI Y Z, et al. Growth of Bacillus cereus in nutritional broth[J]. Food Research and Development, 2009, 30(9): 69−72.
15
RAUH V M, JOHANSEN L B, IPSEN R, et al. Plasmin activity in UHT milk: Relationship between proteolysis, age gelation, and bitterness[J]. Journal of Agricultural and Food Chemistry, 2014, 62(28): 6852−6860.
16
王慧莹. 基于计算机视觉技术快速检测食品中蜡样芽孢杆菌的研究[D]. 长春: 吉林大学, 2011. [WANG H Y. Research on rapid detection of Bacillus cereus in food by computer vision[D]. Changchun: Jilin University, 2011.]
WANG H Y. Research on rapid detection of Bacillus cereus in food by computer vision[D]. Changchun: Jilin University, 2011.
17
YANG Y, WEI S, ZHONG J, et al. Exposure assessment of Bacillus cereus introduced into reconstituted milk production in China[J]. Food Bioscience, 2024, 59: 104024.
18
牛犇, 洪斌, 穆丽丽, 等. 荧光定量PCR在预测微生物学中的应用[J]. 食品工业科技, 2019, 40(5): 275−282. [NIU B, HONG B, MU L L, et al. Application of fluorescent quantitative PCR in predictive microbiology[J]. Science and Technology of Food Industry, 2019, 40(5): 275−282.]
NIU B, HONG B, MU L L, et al. Application of fluorescent quantitative PCR in predictive microbiology[J]. Science and Technology of Food Industry, 2019, 40(5): 275−282.
19
RAUH V M I R D E. Plasmin and bacterial protease interactions in UHT milk during storage[J]. Journal of Agricultural and Food Chemistry, 2020, 68(12): 3564−3572.
20
屈雅莉, 张彦辉, 夏兆田, 等. 分离自生牛乳的一株高产蛋白酶蜡样芽孢杆菌特性[J]. 乳业科学与技术, 2025, 48(3): 55−62. [QU Y L, ZHANG Y H, XIA Z T, et al. Characteristics of a protease-producing strain of Bacillus cereus isolated from raw milk[J]. Journal of Dairy Science and Technology, 2025, 48(3): 55−62.]
QU Y L, ZHANG Y H, XIA Z T, et al. Characteristics of a protease-producing strain of Bacillus cereus isolated from raw milk[J]. Journal of Dairy Science and Technology, 2025, 48(3): 55−62.
21
黄立波. VITEK-2全自动微生物鉴定仪在蜡样芽孢杆菌检测中应用分析[J]. 中国医疗器械信息, 2021, 27(6): 42−43. [HUANG L B. VITEK-2 automatic microbial identification instrument was applied in the detection of Bacillus cereus[J]. Information on Chinese medical devices, 2021, 27(6): 42−43.]
HUANG L B. VITEK-2 automatic microbial identification instrument was applied in the detection of Bacillus cereus[J]. Information on Chinese medical devices, 2021, 27(6): 42−43.
22
中华人民共和国国家卫生和计划生育委员会. 食品安全国家标准 食品微生物学检验 蜡样芽孢杆菌检验: GB 4789.14-2014[S]. 北京: 中国标准出版社, 2014. [National Health and Family Planning Commission of the People's Republic of China. National food safety standard: Microbiological examination of food-Bacillus cereus test: GB 4789.14-2014 [S]. Beijing: China Standards Press, 2014.]
National Health and Family Planning Commission of the People's Republic of China. National food safety standard: Microbiological examination of food-Bacillus cereus test: GB 4789.14-2014 [S]. Beijing: China Standards Press, 2014.
23
廖丽辉, 侯艳飞, 秦捷, 等. 蜡样芽孢杆菌根状生长实验方法探讨[J]. 现代食品, 2023, 29(13): 144−147. [LIAO L H, HOU Y F, QIN J, et al. Discussion on the experimental method of Bacillus cereus rhizoid growth[J]. Modern Food, 2023, 29(13): 144−147.]
LIAO L H, HOU Y F, QIN J, et al. Discussion on the experimental method of Bacillus cereus rhizoid growth[J]. Modern Food, 2023, 29(13): 144−147.
24
凌云, 王晶, 王越菲, 等. 不同裂解方式对微生物DNA提取的影响[J]. 江苏农业科学, 2016, 44(12): 61−64. [LING Y, WANG J, WANG Y F, et al. The impact of different lysis methods on microbial DNA extraction[J]. Jiangsu Agricultural Science, 2016, 44(12): 61−64.]
LING Y, WANG J, WANG Y F, et al. The impact of different lysis methods on microbial DNA extraction[J]. Jiangsu Agricultural Science, 2016, 44(12): 61−64.
25
张鑫, 罗雨帆, 许庆方. 一株芽孢杆菌的筛选及其降解木质纤维素性能[J]. 微生物学报, 2025, 65(4): 1512−1528. [ZHANG X, LUO Y F, XU Q F. A Bacillus strain capable of degrading lignocellulose[J]. Acta Microbiologica Sinica, 2025, 65(4): 1512−1528.]
ZHANG X, LUO Y F, XU Q F. A Bacillus strain capable of degrading lignocellulose[J]. Acta Microbiologica Sinica, 2025, 65(4): 1512−1528.
26
ELAHI S, FUJIKAWA H. Comprehensive study of the boundaries of enterotoxin A production and growth of Staphylococcus aureus at various temperatures and salt concentrations[J]. Journal of Food Science, 2019, 84(1): 121−126.
27
陈琛, 李学英, 杨宪时, 等. 环境因子交互作用下蜡样芽孢杆菌生长/非生长界面模型的建立与评价[J]. 现代食品科技, 2015, 31(12): 205−213. [CHEN C, LI X Y, YANG X S, et al. Modeling and evaluating the growth/no growth boundaries of Bacillus cereus: Effect of temperature, pH, and water activity[J]. Modern Food Science & Technology, 2015, 31(12): 205−213.]
CHEN C, LI X Y, YANG X S, et al. Modeling and evaluating the growth/no growth boundaries of Bacillus cereus: Effect of temperature, pH, and water activity[J]. Modern Food Science & Technology, 2015, 31(12): 205−213.
28
MEMBRE J, LAMBERT R J W. Application of predictive modelling techniques in industry: from food design up to risk assessment[J]. International Journal of Food Microbiology, 2008, 128(1): 10−15.
29
中华人民共和国卫生部. 食品安全国家标准 生乳: GB 19301-2010[S]. 北京: 中国标准出版社, 2010. [Ministry of Health of the People's Republic of China. National food safety standards for raw milk: GB 19301-2010[S]. Beijing: China Standards Press, 2010.]
Ministry of Health of the People's Republic of China. National food safety standards for raw milk: GB 19301-2010[S]. Beijing: China Standards Press, 2010.
30
邵景海, 王占东, 王青云, 等. 双蛋白有机酸奶产品的开发[J]. 食品安全导刊, 2021(22): 93−95. [SHAO J H, WANG Z D, WANG Q Y, et al. Development of double-protein organic yogurt products[J]. China Food Safety Magazine, 2021(22): 93−95.]
SHAO J H, WANG Z D, WANG Q Y, et al. Development of double-protein organic yogurt products[J]. China Food Safety Magazine, 2021(22): 93−95.
31
乔霖楠, 刘阳泰, 王晔茹, 等. 中国食品中蜡样芽孢杆菌污染状况荟萃分析[J]. 工业微生物, 2025, 55(1): 155−160. [QIAO L N, LIU Y T, WANG Y R, et al. A meta-analysis of Bacillus cereus prevalence in Chinese foods[J]. Industrial Microbiology, 2025, 55(1): 155−160.]
QIAO L N, LIU Y T, WANG Y R, et al. A meta-analysis of Bacillus cereus prevalence in Chinese foods[J]. Industrial Microbiology, 2025, 55(1): 155−160.
32
BARANYI J, ROBERTS T A. Mathematics of predictive food microbiology[J]. International Journal of Food Microbiology, 1995, 26(2): 199−218.
33
KYRITSI M, TSIOLAS G, TSOUREKI A, et al. Genomic and transcriptomic profiling of Bacillus cereus in milk: Insights into the sweet curdling defect[J]. Foods, 2025, 14(5): 780.
34
AGUILAR C E G, ROSSI G A M, SILVA H O, et al. Gene detection and enzymatic activity of psychrotrophic Bacillus cereus s. isolated from milking environments, dairies, milk, and dairy products[J]. Microorganisms, 2025, 13(4): 889.
2026年第47卷第17期
PDF下载
91
40
引用本文
BibTeX
文章信息
doi: 10.13386/j.issn1002-0306.2025090242
  • 接收时间:2025-09-22
  • 首发时间:2026-10-24
  • 出版时间:2026-09-01
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期:2025-09-22
基金
作者信息
    1西南民族大学药学与食品学院,四川成都 610225
    2康美包(苏州)有限公司,江苏苏州 215021

通讯作者:

唐俊妮(1971−),女,博士,教授,研究方向:食品微生物与食品安全,E-mail:。
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/spgykj/CN/10.13386/j.issn1002-0306.2025090242
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏