Article(id=1320176032066425148, tenantId=1146029695717560320, journalId=1260987677001138203, issueId=1320175721343987874, articleNumber=null, orderNo=null, doi=10.13386/j.issn1002-0306.2025060087, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1749398400000, receivedDateStr=2025-06-09, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1792684093729, onlineDateStr=2026-10-22, pubDate=1781452800000, pubDateStr=2026-06-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1792684093729, onlineIssueDateStr=2026-10-22, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1792684093729, creator=13701087609, updateTime=1792684093729, updator=13701087609, issue=Issue{id=1320175721343987874, tenantId=1146029695717560320, journalId=1260987677001138203, year='2026', volume='47', issue='12', pageStart='1', pageEnd='517', issueExtLink='null', onlineDate='null', pubDate='1781452800000', pubDateStr='2026-06-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1792684019647, creator='13701087609', updateTime=1792684019647, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=412, endPage=419, ext={EN=ArticleExt(id=1320176032271946045, articleId=1320176032066425148, tenantId=1146029695717560320, journalId=1260987677001138203, language=EN, title=Effect of Plasma Activated Water Synergistic Organic Acids on Antibacterial Effect and Quality of Chilled Chicken during Storage, columnId=1261343845820805453, journalTitle=Science and Technology of Food Industry, columnName=Storage and Preservation, runingTitle=null, highlight=null, articleAbstract=

In order to investigate the novel method to extend the shelf life of chilled chicken, thereby addressing the bottleneck restricting that constrains high-quality development of the industry, the antibacterial effect and storage quality at 4 ℃of plasma activated water (PAW) and organic acids (0.2% citric acid, 0.2% lactic acid solution) on chilled chicken were explored in the current investigation. The results showed that PAW synergistic organic acid treatment effectively inhibited the increase of total viable count (TVC) and coliform population of chilled chickens (P<0.05). It reduced the initial TVC by 0.77~1.21 lg CFU/g and the coliform bacteria count by 0.07~0.57 lg CFU/g. During storage, the PAW synergistic organic acid treatment inhibited the increase of the pH, K value and malondialdehyde content of chilled chickens (P<0.05). In the early stage of storage (0~8 d), PAW synergistic organic acid treatment had not an adverse effect on the color. The synergistic treatment of PAW with organic acid reduced the shear force of the sample, but not significant (P>0.05). Although PAW and organic acid treatment increased the nitrite content of chilled chickens (P<0.05), to a maximum of 21.43 mg/kg, which was still within the safe range and would not cause food safety issues. In conclusion, compared with NaClO treatment, PAW and PAW synergistic organic acids treatment effectively extended the shelf life of chilled chicken by 8 and 12 d, and maintain storage quality. This study could provide a theoretical basis for the application of PAW synergistic organic acids in the preservation of chilled chicken.

, authors=Xinyi LI1, 2, Shuangshuang JIN3, Mingyue WANG1, 2, Shaolin DENG2, 3, Xiaohui KONG3, Xiaoming WANG3, Xinglian XU1, 2, Minyi HAN1, 2, 3, *, authorsList=Xinyi LI, Shuangshuang JIN, Mingyue WANG, Shaolin DENG, Xiaohui KONG, Xiaoming WANG, Xinglian XU, Minyi HAN, authorCompany=null, correspAuthors=Minyi HAN, authorNote=null, correspAuthorsNote=null, copyrightStatement=Copyright © 2026 Science and Technology of Food Industry. All rights reserved., copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1320176033492488520, articleId=1320176032066425148, tenantId=1146029695717560320, journalId=1260987677001138203, language=CN, title=等离子体活化水协同有机酸对冰鲜鸡抑菌效果及贮藏品质的影响, columnId=1261343846517059923, journalTitle=食品工业科技, columnName=贮运保鲜, runingTitle=null, highlight=null, articleAbstract=

本文旨在探索延长冰鲜鸡货架期的新方法,解决制约行业高质量发展的瓶颈问题。现以冰鲜鸡为研究对象,探究等离子体活化水(plasma activated water,PAW)协同有机酸(0.2%柠檬酸、0.2%乳酸溶液)对冰鲜鸡的抑菌效果及4 ℃条件下贮藏品质的影响。结果表明,等离子体活化水协同有机酸处理能有效抑制冰鲜鸡菌落总数及大肠菌群数的增长(P<0.05),可使初始菌落总数降低0.77~1.21 lg CFU/g、大肠菌群数降低0.07~0.57 lg CFU/g。在贮藏期间,等离子体活化水协同有机酸处理能显著抑制冰鲜鸡pH、K值、丙二醛含量的增长(P<0.05);在贮藏前期(0~8 d),等离子体活化水协同有机酸处理未对肉色造成不良影响。等离子体活化水协同有机酸处理能降低鸡肉剪切力但并不显著(P>0.05)。虽然等离子体活化水协同有机酸处理使冰鲜鸡亚硝酸盐含量显著增加(P<0.05),最高为21.43 mg/kg,但仍在安全范围内,不会引发食品安全问题。综上所述,与NaClO处理相比,PAW、PAW协同有机酸处理有效延长冰鲜鸡货架期至8、12 d,维持贮藏品质。本研究可为PAW协同有机酸在冰鲜鸡保鲜方面的应用提供理论依据。

, authors=李欣怡1, 2, 靳爽爽3, 王明月1, 2, 邓绍林2, 3, 孔晓慧3, 王晓明3, 徐幸莲1, 2, 韩敏义1, 2, 3, *, authorsList=李欣怡, 靳爽爽, 王明月, 邓绍林, 孔晓慧, 王晓明, 徐幸莲, 韩敏义, authorCompany=null, correspAuthors=韩敏义, authorNote=

李欣怡(2001−),女,硕士研究生,研究方向:肉品加工与质量控制,E-mail:

, correspAuthorsNote=
韩敏义(1975−),男,博士,研究员,研究方向:肉品加工与质量控制,E-mail:。
, copyrightStatement=版权所有 © 2026《食品工业科技》编辑部, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=jMgSyxhLju4k6YnOrS67TQ==, magXml=na0LJoVJbZAj5xoGZj3FSg==, pdfUrl=null, pdf=m7MeEjl8WOzs/7f/VYI+PQ==, pdfFileSize=1502870, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=OwbWzi/uMap3bUu1xsa78Q==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=ZzjvwRECXx/7URQoOvM9Eg==, mapNumber=null, fund=null)}, authors=[Author(id=1320176035392508243, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=lixinyi@stu.njau.edu.cn, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320176035522531670, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, authorId=1320176035392508243, language=EN, stringName=Xinyi LI, firstName=Xinyi, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, address=1Yunfu Branch of Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, Yunfu 527400, China
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Food Science, 2025, 46(2): 20−29.], articleTitle=null, refAbstract=null), Reference(id=1320176049120465361, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=44, rfOrder=53, authorNames=null, journalName=null, refType=null, unstructuredReference=KE Zhigang, TIAN Yuling, LIU Shulai, et al. Effect of plasma-activated water combined with biological preservatives on beef preservation[J]. 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Molecules, 2022, 27(19): 6703., articleTitle=null, refAbstract=null)], funds=null, companyList=[AuthorCompany(id=1320176033714786633, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, xref=1, ext=[AuthorCompanyExt(id=1320176033727369546, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, companyId=1320176033714786633, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1Yunfu Branch of Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, Yunfu 527400, China), AuthorCompanyExt(id=1320176033735758155, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, companyId=1320176033714786633, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1岭南现代农业科学与技术广东省实验室云浮分中心,广东云浮 527400)]), AuthorCompany(id=1320176035203764556, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, xref=2, ext=[AuthorCompanyExt(id=1320176035212153165, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, companyId=1320176035203764556, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2College of Food Science and Technology, Nanjing Agricultural University, National Engineering Research Center for Meat Quality and Safety Control, Nanjing 210095, China), AuthorCompanyExt(id=1320176035224736078, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, companyId=1320176035203764556, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2南京农业大学食品科技学院,国家肉品质量安全控制工程技术研究中心,江苏南京 210095)]), AuthorCompany(id=1320176035296039247, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, xref=3, ext=[AuthorCompanyExt(id=1320176035304427856, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, companyId=1320176035296039247, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3Wen's Food Group Co., Ltd., Yunfu 527400, China), AuthorCompanyExt(id=1320176035308622161, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, companyId=1320176035296039247, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3温氏食品集团股份有限公司,广东云浮 527400)])], figs=[ArticleFig(id=1320176039838470538, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=EN, label=Fig.1, caption=Changes of total viable count (A) and coliforms (B) of chilled chicken with different treatment during storage, figureFileSmall=YGmFHGJIIg0r47p4F1QI8w==, figureFileBig=OwbWzi/uMap3bUu1xsa78Q==, tableContent=null), ArticleFig(id=1320176040140460427, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=CN, label=图1, caption=不同处理组冰鲜鸡贮藏期内菌落总数(A)和大肠菌群(B)的变化

注:图中不同大写字母表示同一时间内不同处理组之间差异显著,不同小写字母表示不同时间内同一处理组之间差异显著(P<0.05),图2~图5同。

, figureFileSmall=YGmFHGJIIg0r47p4F1QI8w==, figureFileBig=OwbWzi/uMap3bUu1xsa78Q==, tableContent=null), ArticleFig(id=1320176040371147148, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=EN, label=Fig.2, caption=Changes in pH of chilled chicken with different treatment during storage, figureFileSmall=zBAcUF3sXFXAULIrREBflA==, figureFileBig=ck8/+5lcS3XYFLLBq2yFPg==, tableContent=null), ArticleFig(id=1320176040576668045, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=CN, label=图2, caption=不同处理组冰鲜鸡贮藏期内pH的变化, figureFileSmall=zBAcUF3sXFXAULIrREBflA==, figureFileBig=ck8/+5lcS3XYFLLBq2yFPg==, tableContent=null), ArticleFig(id=1320176040694108558, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=EN, label=Fig.3, caption=Changes in K value of chilled chicken with different treatment during storage, figureFileSmall=nXtQH8PKaOm0HWy5G3xUyw==, figureFileBig=EZOFuF7j1+f+ihg0csr44Q==, tableContent=null), ArticleFig(id=1320176040970932623, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=CN, label=图3, caption=不同处理组冰鲜鸡贮藏期内K值的变化, figureFileSmall=nXtQH8PKaOm0HWy5G3xUyw==, figureFileBig=EZOFuF7j1+f+ihg0csr44Q==, tableContent=null), ArticleFig(id=1320176041054818704, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=EN, label=Fig.4, caption=Changes in malondialdehyde of chilled chickenwith different treatment during storage, figureFileSmall=JqcfJz3Z4KjmCBQvzYoa0Q==, figureFileBig=gcHX4bGrtcyTxlUFgJq5jg==, tableContent=null), ArticleFig(id=1320176041147093393, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=CN, label=图4, caption=不同处理组冰鲜鸡贮藏期内丙二醛的变化, figureFileSmall=JqcfJz3Z4KjmCBQvzYoa0Q==, figureFileBig=gcHX4bGrtcyTxlUFgJq5jg==, tableContent=null), ArticleFig(id=1320176041226785170, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=EN, label=Fig.5, caption=Changes in nitrite of chilled chicken with different treatment during storage, figureFileSmall=Q/gVxrwDB0JjAHMIyw6OOQ==, figureFileBig=psGAnLYCLFYjfmoSLgrsNA==, tableContent=null), ArticleFig(id=1320176041298088339, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=CN, label=图5, caption=不同处理组冰鲜鸡贮藏期内亚硝酸盐的变化, figureFileSmall=Q/gVxrwDB0JjAHMIyw6OOQ==, figureFileBig=psGAnLYCLFYjfmoSLgrsNA==, tableContent=null), ArticleFig(id=1320176041365197204, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=EN, label=Table 1, caption=

Standards for sensory evaluation of chilled chicken

, figureFileSmall=null, figureFileBig=null, tableContent=
指标得分标准
8~10(分)5~7(分)1~4(分)
色泽色泽正常,有光泽色泽暗淡,光泽度差色泽暗淡,无光泽
气味正常鸡肉气味鸡肉味淡,微异味鸡肉味消失,有异味
表面组织状态外表微干、不粘手外表干燥、微粘手粘手
整体接受性可接受度高可接受度一般不可接受
), ArticleFig(id=1320176041423917461, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=CN, label=表1, caption=

冰鲜鸡肉的感官评分标准

, figureFileSmall=null, figureFileBig=null, tableContent=
指标得分标准
8~10(分)5~7(分)1~4(分)
色泽色泽正常,有光泽色泽暗淡,光泽度差色泽暗淡,无光泽
气味正常鸡肉气味鸡肉味淡,微异味鸡肉味消失,有异味
表面组织状态外表微干、不粘手外表干燥、微粘手粘手
整体接受性可接受度高可接受度一般不可接受
), ArticleFig(id=1320176041511997846, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=EN, label=Table 2, caption=

Changes in colour of chilled chicken with different treatment during storage

, figureFileSmall=null, figureFileBig=null, tableContent=
指标处理条件贮藏时间(d)
02468101214
L*NaClO73.38±0.39Aa72.93±0.91Aa72.87±0.33Ba72.46±0.98Aab72.29±0.44Abc70.86±0.82Ac69.26±0.73Ad67.55±1.51Ae
PAW73.65±0.58Aa73.25±0.43Aa73.15±0.64Aab72.91±0.52Aab72.42±0.61Abc71.28±0.51Ac70.50±1.16Ad68.58±1.21Ae
PACA73.73±0.83Aa73.4±0.66Aa73.63±0.60Aa72.99±0.29Aa72.49±0.46Ab71.20±1.04Ab69.86±1.12Ac68.73±1.42Ad
PALA73.46±0.61Aa73.6±0.64Aa73.69±0.52Aa72.92±0.34Aa72.52±0.86Ab71.27±1.20Ab70.37±1.12Ab68.65±1.40Ac
a*NaClO1.37±0.21Aa1.24±0.17Aa1.32±0.36Aa1.17±0.12Aa0.83±0.23Ab0.74±0.18Ab0.71±0.15Abc0.49±0.35Ac
PAW1.19±0.08Aa1.09±0.09Aab1.04±0.21Aab1.05±0.18Aab0.77±0.25Ab0.71±0.21Ab0.67±0.19Ab0.14±0.75Ac
PACA1.16±0.35Aa1.07±0.12Aa1.05±0.30Aa1.03±0.14Aa0.63±0.28Aa0.11±0.52Bb−0.87±0.51Bc−0.88±0.78Bc
PALA1.01±0.29Aa1.10±0.20Aa1.04±0.11Aa1.05±0.15Aa0.56±0.21Ab0.16±0.53Bc−0.90±0.36Bd−1.09±0.56Bd
b*NaClO1.13±0.15Aa1.20±0.30Aa1.18±0.21Aab1.23±0.37Aab1.26±0.29Aab1.41±0.24Aab1.66±0.31Aab1.70±0.35Aab
PAW1.34±0.40Ab1.32±0.31Ab1.30±0.27Ab1.38±0.28Aab1.42±0.25Aab1.52±0.32Aab1.72±0.41Aa1.78±0.44Aa
PACA1.11±0.27Ac1.26±0.34Aab1.22±0.24Abc1.31±0.19Aa1.35±0.14Aa1.45±0.29Aa1.75±0.33Aa1.81±0.38Aa
PALA1.19±0.28Ab1.25±0.21Aab1.24±0.17Aab1.27±0.24Aab1.39±0.21Aa1.54±0.24Aa1.73±0.30Aa1.82±0.53Aa
), ArticleFig(id=1320176041608466839, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=CN, label=表2, caption=

不同处理组冰鲜鸡贮藏期内色泽的变化

, figureFileSmall=null, figureFileBig=null, tableContent=
指标处理条件贮藏时间(d)
02468101214
L*NaClO73.38±0.39Aa72.93±0.91Aa72.87±0.33Ba72.46±0.98Aab72.29±0.44Abc70.86±0.82Ac69.26±0.73Ad67.55±1.51Ae
PAW73.65±0.58Aa73.25±0.43Aa73.15±0.64Aab72.91±0.52Aab72.42±0.61Abc71.28±0.51Ac70.50±1.16Ad68.58±1.21Ae
PACA73.73±0.83Aa73.4±0.66Aa73.63±0.60Aa72.99±0.29Aa72.49±0.46Ab71.20±1.04Ab69.86±1.12Ac68.73±1.42Ad
PALA73.46±0.61Aa73.6±0.64Aa73.69±0.52Aa72.92±0.34Aa72.52±0.86Ab71.27±1.20Ab70.37±1.12Ab68.65±1.40Ac
a*NaClO1.37±0.21Aa1.24±0.17Aa1.32±0.36Aa1.17±0.12Aa0.83±0.23Ab0.74±0.18Ab0.71±0.15Abc0.49±0.35Ac
PAW1.19±0.08Aa1.09±0.09Aab1.04±0.21Aab1.05±0.18Aab0.77±0.25Ab0.71±0.21Ab0.67±0.19Ab0.14±0.75Ac
PACA1.16±0.35Aa1.07±0.12Aa1.05±0.30Aa1.03±0.14Aa0.63±0.28Aa0.11±0.52Bb−0.87±0.51Bc−0.88±0.78Bc
PALA1.01±0.29Aa1.10±0.20Aa1.04±0.11Aa1.05±0.15Aa0.56±0.21Ab0.16±0.53Bc−0.90±0.36Bd−1.09±0.56Bd
b*NaClO1.13±0.15Aa1.20±0.30Aa1.18±0.21Aab1.23±0.37Aab1.26±0.29Aab1.41±0.24Aab1.66±0.31Aab1.70±0.35Aab
PAW1.34±0.40Ab1.32±0.31Ab1.30±0.27Ab1.38±0.28Aab1.42±0.25Aab1.52±0.32Aab1.72±0.41Aa1.78±0.44Aa
PACA1.11±0.27Ac1.26±0.34Aab1.22±0.24Abc1.31±0.19Aa1.35±0.14Aa1.45±0.29Aa1.75±0.33Aa1.81±0.38Aa
PALA1.19±0.28Ab1.25±0.21Aab1.24±0.17Aab1.27±0.24Aab1.39±0.21Aa1.54±0.24Aa1.73±0.30Aa1.82±0.53Aa
), ArticleFig(id=1320176041688158616, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=EN, label=Table 3, caption=

Changes in shear force of chilled chicken with different treatment during storage

, figureFileSmall=null, figureFileBig=null, tableContent=
指标处理方式贮藏时间(d)
02468101214
剪切力(N)NaClO22.87±0.93Aa19.84±0.70Ab17.88±1.20Ac16.64±0.91Ad15.98±0.90Ade15.10±0.63Aef13.72±1.28Bf12.45±0.85Bg
PAW22.63±0.72Aa19.23±0.65Ab17.14±0.73Ac16.16±0.92Ad15.75±0.58Ade15.07±0.56Aef14.55±0.51ABf13.58±0.60Ag
PACA22.42±0.58Aa19.18±0.91Ab17.11±0.70Ac16.01±0.77Ad15.74±0.39Ad15.00±0.51Ae14.80±0.93Ae13.64±0.71Af
PALA22.55±0.88Aa19.07±1.42Ab17.13±0.63Ac15.92±0.75Ad15.57±0.57Ad15.05±0.67Ade14.65±0.63ABe13.70±0.73Af
), ArticleFig(id=1320176041763656089, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=CN, label=表3, caption=

不同处理组冰鲜鸡贮藏期内剪切力的变化

, figureFileSmall=null, figureFileBig=null, tableContent=
指标处理方式贮藏时间(d)
02468101214
剪切力(N)NaClO22.87±0.93Aa19.84±0.70Ab17.88±1.20Ac16.64±0.91Ad15.98±0.90Ade15.10±0.63Aef13.72±1.28Bf12.45±0.85Bg
PAW22.63±0.72Aa19.23±0.65Ab17.14±0.73Ac16.16±0.92Ad15.75±0.58Ade15.07±0.56Aef14.55±0.51ABf13.58±0.60Ag
PACA22.42±0.58Aa19.18±0.91Ab17.11±0.70Ac16.01±0.77Ad15.74±0.39Ad15.00±0.51Ae14.80±0.93Ae13.64±0.71Af
PALA22.55±0.88Aa19.07±1.42Ab17.13±0.63Ac15.92±0.75Ad15.57±0.57Ad15.05±0.67Ade14.65±0.63ABe13.70±0.73Af
), ArticleFig(id=1320176041839153562, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=EN, label=Table 4, caption=

Changes in sensory scores of chilled chicken with different treatment during storage

, figureFileSmall=null, figureFileBig=null, tableContent=
指标处理方式贮藏时间(d)
02468101214
色泽NaClO9.67±0.52Aa9.00±0.89Aa7.33±0.82Bb5.33±0.82Bc4.67±1.03Bc3.33±0.82Cd2.33±0.82Ce1.83±0.98Be
PAW9.83±0.41Aa9.33±0.52Aa8.00±0.63ABb7.67±0.52Ab7.17±0.75Ab5.33±1.03Bc3.50±1.05Bd1.50±0.55Be
PACA9.83±0.41Aa9.17±0.75Aab8.33±0.52Ab7.33±0.82Ac7.33±0.82Ac7.00±0.89Acd6.17±0.75Ade5.50±1.04Ae
PALA9.83±0.41Aa9.17±0.75Aa8.17±0.41Ab7.50±0.55Abc7.67±0.52Abc7.17±0.75Ac6.00±0.63Ad6.17±0.98Ad
气味NaClO9.50±0.55Aa8.33±0.52Ab5.17±1.17Cc3.33±0.82Cd3.00±0.89Cd2.50±0.55Cd1.50±0.55Ce1.00±0.00Be
PAW10.00±0.00Aa9.00±0.63Ab7.33±0.82Bc5.33±1.21Bd4.67±0.82Bde3.83±0.75Be3.00±0.63Bf1.50±0.55Bf
PACA10.00±0.00Aa9.50±0.55Aa8.67±0.52Ab8.00±0.63Abc7.67±0.52Ac6.00±0.89Ad5.17±0.75Ae3.50±0.55Ae
PALA10.00±0.00Aa9.33±0.52Aa8.50±0.55Ab8.17±0.75Abc7.50±0.55Ac6.17±0.75Ad5.17±0.75Ae3.67±0.82Ae
表面组织状态NaClO10.00±0.00Aa9.17±0.75Aa7.83±0.72Bb6.17±0.75Bc4.50±1.05Cd3.83±0.75Cd2.50±1.05Be1.50±0.55Cf
PAW10.00±0.00Aa9.50±0.55Aab8.83±0.75Ab7.50±0.55Ac6.00±0.63Bd5.17±0.75Be4.17±0.75Af3.17±0.55Bg
PACA10.00±0.00Aa9.33±0.52Aab9.00±0.63Ab7.67±0.82Ac7.17±0.75Ac6.17±0.55Ad5.16±0.72Ae4.83±0.75Ae
PALA10.00±0.00Aa9.50±0.55Aab9.00±0.63Ab7.83±0.41Ac6.83±0.75ABd6.33±0.52Ad5.17±0.75Ae4.67±0.82Ae
整体接受性NaClO10.00±0.00Aa8.83±0.75Ab5.67±0.82Bc3.83±0.75Cd3.83±0.72Cd1.83±0.75Ce1.33±0.52Bef1.00±0.00Cf
PAW10.00±0.00Aa8.83±0.75Ab8.33±0.52Ab6.83±0.41Bc5.50±1.05Bd4.50±1.05Be4.17±0.75Ae2.67±0.82Bf
PACA10.00±0.00Aa9.00±0.63Ab8.83±0.75Ab7.33±0.82ABc8.00±0.63Ac7.17±0.41Ac5.33±1.03Ad4.33±0.82Ae
PALA10.00±0.00Aa9.00±0.63Ab8.67±0.52Ab7.83±0.75Ac7.67±0.52Ac7.17±0.75Ac5.00±0.89Ad4.83±0.75Ad
), ArticleFig(id=1320176041910456731, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320176032066425148, language=CN, label=表4, caption=

不同处理组冰鲜鸡贮藏期内感官评分的变化

, figureFileSmall=null, figureFileBig=null, tableContent=
指标处理方式贮藏时间(d)
02468101214
色泽NaClO9.67±0.52Aa9.00±0.89Aa7.33±0.82Bb5.33±0.82Bc4.67±1.03Bc3.33±0.82Cd2.33±0.82Ce1.83±0.98Be
PAW9.83±0.41Aa9.33±0.52Aa8.00±0.63ABb7.67±0.52Ab7.17±0.75Ab5.33±1.03Bc3.50±1.05Bd1.50±0.55Be
PACA9.83±0.41Aa9.17±0.75Aab8.33±0.52Ab7.33±0.82Ac7.33±0.82Ac7.00±0.89Acd6.17±0.75Ade5.50±1.04Ae
PALA9.83±0.41Aa9.17±0.75Aa8.17±0.41Ab7.50±0.55Abc7.67±0.52Abc7.17±0.75Ac6.00±0.63Ad6.17±0.98Ad
气味NaClO9.50±0.55Aa8.33±0.52Ab5.17±1.17Cc3.33±0.82Cd3.00±0.89Cd2.50±0.55Cd1.50±0.55Ce1.00±0.00Be
PAW10.00±0.00Aa9.00±0.63Ab7.33±0.82Bc5.33±1.21Bd4.67±0.82Bde3.83±0.75Be3.00±0.63Bf1.50±0.55Bf
PACA10.00±0.00Aa9.50±0.55Aa8.67±0.52Ab8.00±0.63Abc7.67±0.52Ac6.00±0.89Ad5.17±0.75Ae3.50±0.55Ae
PALA10.00±0.00Aa9.33±0.52Aa8.50±0.55Ab8.17±0.75Abc7.50±0.55Ac6.17±0.75Ad5.17±0.75Ae3.67±0.82Ae
表面组织状态NaClO10.00±0.00Aa9.17±0.75Aa7.83±0.72Bb6.17±0.75Bc4.50±1.05Cd3.83±0.75Cd2.50±1.05Be1.50±0.55Cf
PAW10.00±0.00Aa9.50±0.55Aab8.83±0.75Ab7.50±0.55Ac6.00±0.63Bd5.17±0.75Be4.17±0.75Af3.17±0.55Bg
PACA10.00±0.00Aa9.33±0.52Aab9.00±0.63Ab7.67±0.82Ac7.17±0.75Ac6.17±0.55Ad5.16±0.72Ae4.83±0.75Ae
PALA10.00±0.00Aa9.50±0.55Aab9.00±0.63Ab7.83±0.41Ac6.83±0.75ABd6.33±0.52Ad5.17±0.75Ae4.67±0.82Ae
整体接受性NaClO10.00±0.00Aa8.83±0.75Ab5.67±0.82Bc3.83±0.75Cd3.83±0.72Cd1.83±0.75Ce1.33±0.52Bef1.00±0.00Cf
PAW10.00±0.00Aa8.83±0.75Ab8.33±0.52Ab6.83±0.41Bc5.50±1.05Bd4.50±1.05Be4.17±0.75Ae2.67±0.82Bf
PACA10.00±0.00Aa9.00±0.63Ab8.83±0.75Ab7.33±0.82ABc8.00±0.63Ac7.17±0.41Ac5.33±1.03Ad4.33±0.82Ae
PALA10.00±0.00Aa9.00±0.63Ab8.67±0.52Ab7.83±0.75Ac7.67±0.52Ac7.17±0.75Ac5.00±0.89Ad4.83±0.75Ad
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李欣怡 1, 2 , 靳爽爽 3 , 王明月 1, 2 , 邓绍林 2, 3 , 孔晓慧 3 , 王晓明 3 , 徐幸莲 1, 2 , 韩敏义 1, 2, 3, *
食品工业科技 | 贮运保鲜 2026,47(12): 412-419
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食品工业科技 |贮运保鲜 2026 , 47 (12) : 412 -419
等离子体活化水协同有机酸对冰鲜鸡抑菌效果及贮藏品质的影响
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2南京农业大学食品科技学院,国家肉品质量安全控制工程技术研究中心,江苏南京 210095, bio={"content":"

李欣怡(2001−),女,硕士研究生,研究方向:肉品加工与质量控制,E-mail:

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李欣怡(2001−),女,硕士研究生,研究方向:肉品加工与质量控制,E-mail:

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李欣怡1, 2 , 靳爽爽3, 王明月1, 2, 邓绍林2, 3, 孔晓慧3, 王晓明3, 徐幸莲1, 2, 韩敏义1, 2, 3, *
作者信息
  • 1岭南现代农业科学与技术广东省实验室云浮分中心,广东云浮 527400
  • 2南京农业大学食品科技学院,国家肉品质量安全控制工程技术研究中心,江苏南京 210095
  • 3温氏食品集团股份有限公司,广东云浮 527400
通讯作者:
韩敏义(1975−),男,博士,研究员,研究方向:肉品加工与质量控制,E-mail:。
作者简介:

李欣怡(2001−),女,硕士研究生,研究方向:肉品加工与质量控制,E-mail:

Effect of Plasma Activated Water Synergistic Organic Acids on Antibacterial Effect and Quality of Chilled Chicken during Storage
Xinyi LI1, 2 , Shuangshuang JIN3, Mingyue WANG1, 2, Shaolin DENG2, 3, Xiaohui KONG3, Xiaoming WANG3, Xinglian XU1, 2, Minyi HAN1, 2, 3, *
Affiliations
  • 1Yunfu Branch of Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, Yunfu 527400, China
  • 2College of Food Science and Technology, Nanjing Agricultural University, National Engineering Research Center for Meat Quality and Safety Control, Nanjing 210095, China
  • 3Wen's Food Group Co., Ltd., Yunfu 527400, China
出版时间: 2026-06-15 doi: 10.13386/j.issn1002-0306.2025060087
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本文旨在探索延长冰鲜鸡货架期的新方法,解决制约行业高质量发展的瓶颈问题。现以冰鲜鸡为研究对象,探究等离子体活化水(plasma activated water,PAW)协同有机酸(0.2%柠檬酸、0.2%乳酸溶液)对冰鲜鸡的抑菌效果及4 ℃条件下贮藏品质的影响。结果表明,等离子体活化水协同有机酸处理能有效抑制冰鲜鸡菌落总数及大肠菌群数的增长(P<0.05),可使初始菌落总数降低0.77~1.21 lg CFU/g、大肠菌群数降低0.07~0.57 lg CFU/g。在贮藏期间,等离子体活化水协同有机酸处理能显著抑制冰鲜鸡pH、K值、丙二醛含量的增长(P<0.05);在贮藏前期(0~8 d),等离子体活化水协同有机酸处理未对肉色造成不良影响。等离子体活化水协同有机酸处理能降低鸡肉剪切力但并不显著(P>0.05)。虽然等离子体活化水协同有机酸处理使冰鲜鸡亚硝酸盐含量显著增加(P<0.05),最高为21.43 mg/kg,但仍在安全范围内,不会引发食品安全问题。综上所述,与NaClO处理相比,PAW、PAW协同有机酸处理有效延长冰鲜鸡货架期至8、12 d,维持贮藏品质。本研究可为PAW协同有机酸在冰鲜鸡保鲜方面的应用提供理论依据。

等离子体活化水  /  有机酸  /  冰鲜鸡  /  贮藏品质  /  货架期

In order to investigate the novel method to extend the shelf life of chilled chicken, thereby addressing the bottleneck restricting that constrains high-quality development of the industry, the antibacterial effect and storage quality at 4 ℃of plasma activated water (PAW) and organic acids (0.2% citric acid, 0.2% lactic acid solution) on chilled chicken were explored in the current investigation. The results showed that PAW synergistic organic acid treatment effectively inhibited the increase of total viable count (TVC) and coliform population of chilled chickens (P<0.05). It reduced the initial TVC by 0.77~1.21 lg CFU/g and the coliform bacteria count by 0.07~0.57 lg CFU/g. During storage, the PAW synergistic organic acid treatment inhibited the increase of the pH, K value and malondialdehyde content of chilled chickens (P<0.05). In the early stage of storage (0~8 d), PAW synergistic organic acid treatment had not an adverse effect on the color. The synergistic treatment of PAW with organic acid reduced the shear force of the sample, but not significant (P>0.05). Although PAW and organic acid treatment increased the nitrite content of chilled chickens (P<0.05), to a maximum of 21.43 mg/kg, which was still within the safe range and would not cause food safety issues. In conclusion, compared with NaClO treatment, PAW and PAW synergistic organic acids treatment effectively extended the shelf life of chilled chicken by 8 and 12 d, and maintain storage quality. This study could provide a theoretical basis for the application of PAW synergistic organic acids in the preservation of chilled chicken.

plasma activated water  /  organic acids  /  chilled chicken  /  storage quality  /  shelf life
李欣怡, 靳爽爽, 王明月, 邓绍林, 孔晓慧, 王晓明, 徐幸莲, 韩敏义. 等离子体活化水协同有机酸对冰鲜鸡抑菌效果及贮藏品质的影响. 食品工业科技, 2026 , 47 (12) : 412 -419 . DOI: 10.13386/j.issn1002-0306.2025060087
Xinyi LI, Shuangshuang JIN, Mingyue WANG, Shaolin DENG, Xiaohui KONG, Xiaoming WANG, Xinglian XU, Minyi HAN. Effect of Plasma Activated Water Synergistic Organic Acids on Antibacterial Effect and Quality of Chilled Chicken during Storage[J]. Science and Technology of Food Industry, 2026 , 47 (12) : 412 -419 . DOI: 10.13386/j.issn1002-0306.2025060087
2024年,我国鸡肉产量为1500万吨,已成为继美国之后的世界第二大鸡肉生产国[1]。近年来,受“禁活”政策以及疫情影响,冰鲜鸡逐渐成为主流消费趋势[2]。然而,冰鲜鸡本身携带的微生物以及环境中的污染源(设备、人员、空气),在屠宰脱毛、掏膛、分割处理等环节侵入组织,并在运输、销售过程中因温度波动而增殖,从而导致其腐败变质甚至引发食品安全问题,严重威胁消费者身体健康,同时也降低产品品质,严重缩短货架期,造成巨大经济损失,阻碍行业高质量发展[3−4]。目前,主要采用的冷藏、添加次氯酸钠、低温等离子体技术等保鲜方法都存在一定的局限性[5],例如,次氯酸钠可能与有机物反应生成致癌物,引发食品安全问题[6];低温等离子体放电过程中产生的部分活性物质会使食物脂质氧化加剧,导致氧化副产物的形成,从而对食品的感官和营养成分产生一定的负面影响、降低消费者对食品的接受度[7−8]。因此,食品行业需要寻求更加绿色、安全、高效的新型抑菌方法,在不损害人体健康的前提下,抑制冰鲜鸡腐败变质、延长货架期、保留冰鲜鸡原有风味特性。
等离子体活化水(Plasma activated water,PAW),是将等离子体发生装置置于水中或水表面进行等离子放电产生[9]。PAW不仅能有效地杀灭食品中的微生物,还能维持食品本身的品质不受显著性影响,并且具有操作简单、处理时间短、效率高等优点[10]。有机酸主要包括柠檬酸、乳酸、乙酸等,由于其具有良好的杀菌效果且安全系数高,已广泛应用于食品行业[11]。然而,单一的PAW或有机酸抑菌效果有限,若将PAW与有机酸联合处理,不仅能增强抑菌效果,还能减少二者使用量,降低成本[12]。Wu等[13]和Zhang等[14]利用PAW与酒石酸联合处理鲜切菠萝及莴苣,可使表面金黄色葡萄球菌分别减少1.92和4.04 lg CFU/g,此外,将PAW与柠檬酸联合使用处理鲜切菠萝抑菌效果也明显提高。Qian等[15]发现,在相同条件下,0.2%乳酸与PAW联合处理能够使牛肉表面沙门氏菌降低3.52 lg CFU/g,而单一的2%乳酸仅降低3.45 lg CFU/g。Kang等[16]将鼠伤寒沙门氏菌接种于鸡胸肉及鸡腿肉表面,采用PAW与乙酸联合处理10 min,鸡胸肉和鸡腿肉表面沙门氏菌分别减少0.98和1.19 lg CFU/g。Jyung等[17]发现有机酸种类不同,协同效果也会表现出差异,甚至不具有协同作用。考虑到乳酸、柠檬酸不仅价格低廉、抑菌效果好且可用于禽类加工,现将同浓度的柠檬酸、乳酸与PAW协同处理,探究能否提高对冰鲜鸡表面微生物的杀菌效果,及其对冰鲜鸡贮藏期间品质的影响。
本文采用PAW、PAW协同有机酸(乳酸、柠檬酸)对冰鲜鸡进行处理,研究不同处理对冰鲜鸡微生物指标(菌落总数、大肠菌群数)、理化指标(pH、色差、丙二醛、K值、亚硝酸盐)及品质(剪切力)的影响,为延长冰鲜鸡货架期以及PAW协同有机酸作为一种新型杀菌技术提供参考。
冰鲜鸡 品种为鲜妃子凤公鸡,45~50日龄,宰后时间为预冷后2 h获得,新兴县温氏佳丰食品有限公司;微生物菌落总数计数测试片、微生物大肠菌群计数测试片 美国3M公司;乳酸、柠檬酸 分析纯,国药集团化学试剂有限公司;高氯酸、氢氧化钾 分析纯,日本QSSOLUTION公司;丙二醛含量检测试剂盒 北京索莱宝生物科技有限公司;食品亚硝酸盐含量测定试剂盒 上海百生跃生物科技有限公司。
PAW-2等离子体活化水设备 苏州屹润食品科技有限公司;SW-CJ-2FD超净工作台 沪净医疗器械上海有限公司;MS 3 basic微型漩涡混合仪 德国IKA公司;DNP-9162恒温培养箱 上海甘易仪器设备有限公司;pH-STAR肉质pH值直测仪 德国麦特斯公司;Chroma Meter CR-400便携式色差仪 日本Konica Minolta公司;C-LM3B数显式肌肉嫩度仪 哈尔滨东北农业大学工程学院;QS-3201鲜度仪 日本QSSOLUTION公司;Infinite200 Pro多功能酶标仪 瑞士帝肯公司;SYG-A2-4电热恒温水浴锅 天津市泰斯特仪器有限公司;UV-6100紫外可见光分光光度计 上海元析有限公司;5810R高速冷冻离心机、5425R高速离心机 德国艾本德公司。
本研究共设四个处理组,分别为NaClO(NaClO浓度为0.005%)、等离子体活化水(PAW)、等离子体活化乳酸(plasma activated lactic acid,PALA)、等离子体活化柠檬酸(plasma activated citric acid,PACA)。
分别配制0.2%(w/v)柠檬酸溶液、0.2%乳酸溶液(v/v)[15],将3L配制的溶液置于等离子体流射装置中,打开等离子体流射装置,处理20 min,处理完成后将其置于密闭容器中冷却至室温。考虑到工厂实际生产情况,料液比1.5:1刚好浸没冰鲜鸡且不造成浪费,将整鸡按照该比例于不同处理组中浸泡15 min后沥干水分,放入无菌袋中,贮存于4 ℃条件下。
冰鲜鸡样品经不同抑菌剂(NaClO、PAW、PALA、PACA)处理15 min后,立即取出装入无菌密封袋中,于4 ℃条件下贮藏14 d,每隔2 d对样品的微生物进行监测。微生物监测采用丁紫璇等[18]的方法,在无菌袋中加入0.9%生理盐水,生理盐水与冰鲜鸡质量比为1:1,将其置于恒温振荡器振荡10 min,转速为300 r/min。对混合液按梯度进行10倍稀释,选取合适梯度,吸取1 mL稀释液滴加于菌落总数测试片和大肠菌群测试片,并于37 ℃恒温培养箱培养48 h。
使用便携式pH计测量鸡肉的pH。在测量前,需要使用pH分别为4.01和7.00的校准液对仪器进行校准。将pH计探头插入鸡胸肉中心,确保探头插入深度一致,待仪器稳定后读数。
使用色差仪评估不同处理组样品的颜色变化。测量前,仪器用标准白板进行校准,观察角为2°,选择C光源。先用滤纸擦拭冰鲜鸡样品表面水分,将孔径为8 mm的探头垂直于样品表面,对冰鲜鸡表皮进行测量。颜色值表示为L*(+亮度,−暗度)、a*(+红色,−绿色)和b*(+黄色,−蓝色)。
取0.2 g鸡胸肉置于2 mL微量离心管中并加入600 μL高氯酸溶液。用剪刀于30 s内快速剪碎样品,再加入氢氧化钾调节样品pH,使得溶液呈中性。将其静置5 min后,取上清液待用。将滤纸固定在电泳槽固定支架上,并将电泳缓冲液均匀喷涂在滤纸上,使滤纸浸透。吸取5 μL上清液滴加在滤纸的指定位置上,推进鲜度仪主机内,在800 V电压条件下,电泳分离5 min。结束后取出滤纸放置于干燥箱干燥2 min。采集影像后,使用K值计算软件即可得到K值。
采用丙二醛检测试剂盒测定不同处理组冰鲜鸡样品的丙二醛含量,称取约0.2 g鸡胸肉,加入2 mL提取液进行冰浴匀浆;于8000 r/min,4 ℃条件下离心10 min,取上清液进行测定。
采用亚硝酸盐含量检测试剂盒测定不同处理组冰鲜鸡样品的亚硝酸盐含量,准确称取0.4 g鸡胸肉,匀浆,加入1 mL提取液一,沸水浴15 min,冷却至室温后,依次加入1 mL提取液二、1 mL提取液三、2 mg试剂三,静置30 min,于8000 r/min,25 ℃条件下离心20 min,取上清液进行测定。
取鸡胸肉剔除表面可见脂肪和肌膜,将其修剪成1 cm×1 cm×3 cm的肉条,置于蒸煮袋中,于80 ℃的水浴锅中加热20 min后取出。利用嫩度计按照垂直于肌纤维方向对样品进行切割,剪切力值单位为(N)。
感官评价参考丁紫璇[19]的方法,评价小组由8名成员组成,从鸡肉的色泽、气味、表面组织状态及整体接受性四个方面进行评价,评价标准如表1所示。
所有样品平行测定3次,数据以平均值±标准差表示。双因素方差分析采用IBM SPSS Statistics 27.0(IBM公司,美国),利用Duncan多重比较对数据的显著性进行分析,显著性水平为0.05。采用Origin 2024(Origin Lab公司,美国)软件绘图。
从图1(A)可知,贮藏第0 d时,与NaClO组相比,冰鲜鸡经PAW、PALA、PACA处理后,表面菌落总数差异显著(P<0.05)。在贮藏期间,NaClO组的菌落总数增长最快,并于第6 d超出GB 16869-2005《鲜、冻禽产品》所规定的临界值6 lg CFU/g,而PAW、PALA、PACA处理组仅为5.68、4.26、3.71 lg CFU/g,远低于NaClO组,其中PACA处理组抑菌效果最佳。从实验结果可知,PAW、PALA、PACA处理组分别于第8、14、14 d超出临界值。由此可见,以NaClO处理组为对照,PAW协同有机酸处理能够延长冰鲜鸡货架期。从微生物方面来看,PAW、PALA、PACA处理能使冰鲜鸡货架期分别延长2、8、8 d。由图1(B)可知,第0 d时,NaClO处理组大肠菌群数为2.82 lg CFU/g,经PAW处理后大肠菌群数仅降低0.07 lg CFU/g,而PALA、PACA处理后,大肠菌群数显著降低至2.25、2.26 lg CFU/g(P<0.05)。随着贮藏时间的延长,PAW、PALA、PACA处理组对大肠菌群均有良好的抑菌效果,其中PACA处理组抑菌效果最佳。研究发现,等离子体活化溶液中的活性氧和活性氮物质具有抑菌作用,氧化应激会降低膜电位,并导致细菌细胞膜破裂,这些物质可以有效抑制肉类在贮藏期间微生物的生长[20]。若将等离子体活化水与有机酸结合,产生的反应性物质可以促进过氧亚硝酸(ONOOH)的形成,ONOOH与H2O2进一步相互作用生成过氧硝酸,从而增强抑菌效果[16,21],这也是PAW协同有机酸抑菌效果更佳的主要原因。综上可知,PAW能有效抑制贮藏期间冰鲜鸡表面菌落总数和大肠菌群数的增长,经有机酸调控后,PACA处理组抑菌效果最佳。
pH是反映肉品新鲜程度的重要指标之一。如图2所示,所有处理组冰鲜鸡样品的pH总体上呈先下降后上升的趋势。屠宰后,肉类中的糖类物质分解产生大量乳酸以及ATP的降解使得pH下降[22]。随着贮藏时间的延长,肉类在内源酶及微生物的作用下,蛋白质分解产生碱性含氮物质导致pH上升[23]。与NaClO组相比,PAW协同有机酸处理组pH显著(P<0.05)降低,造成该现象的原因是PAW协同有机酸溶液中存在大量HNO2、HNO3等酸性物质,这些酸性物质在抑制腐败微生物的同时也使鸡肉pH显著降低[15]。pH在一定程度上可以反映肉品的腐败程度,当pH达到6.20时,肉类开始变质,这与菌落总数变化相对应[24]。由此可见,PAW协同有机酸处理可以有效抑制冰鲜鸡贮藏期间pH的上升,延缓变质。
消费者常将肉色与肉品新鲜程度及品质联系在一起,肉色直接决定了消费者的购买意愿。由表2可知,不同处理组样品的L*值、a*值与b*值在贮藏期的0~8 d无显著差异(P>0.05)。在贮藏末期(12~14 d),PALA、PACA处理组样品的颜色更加偏黄偏绿,其特征是b*值增加、a*值降低变为负值。PAW协同有机酸溶液中的ROS会使肉中肌红蛋白氧化为高铁肌红蛋白,从而导致鸡肉a*值降低[25]。但鸡皮中不含肌红蛋白,故而表皮a*值降低与肌红蛋白变化无关。研究表明,溶液中ROS等强氧化性物质以及低pH对色泽产生不良影响[26–27]。此外,利用有机酸浸泡处理也会造成鸡皮a*值降低、b*值增加[28]。Deumier等[29]研究发现,有机酸处理会导致鸡皮颜色变黄变绿,其中乳酸处理后的b*值高于柠檬酸处理。Kim等[30]的研究也表明乳酸处理会导致鸡翅的a*值降低,在贮藏后期b*值无显著变化。由此推测样品表皮a*值降低可能与溶液性质以及添加有机酸有关。因此,在选择PAW协同有机酸溶液作为鸡肉胴体的消毒剂时,不仅要考虑抑菌效果,还要考虑其对表皮颜色的影响。
由于肉类在屠宰后三磷酸腺苷(ATP)会逐渐转化为磷酸腺苷(ADP)、腺苷酸(AMP)、肌苷酸(IMP)、次黄嘌呤核苷酸(HxR)以及次黄嘌呤(Hx),因此常用ATP降解化合物的相对比例即K值,来预测肉类新鲜程度[31]。由图3可知,随着贮藏时间的延长,冰鲜鸡发生腐败变质,不同处理组样品的K值逐渐增大。贮藏初期(0~2 d),不同处理组冰鲜鸡样品的K值无显著差异(P>0.05)。贮藏至第6 d时,不同处理组冰鲜鸡样品的K值差异显著(P<0.05),这主要是肌苷酸转换为次黄嘌呤和次黄嘌呤苷酸的速度降低导致的[32]。综上可知,等离子体活化水协同有机酸能够抑制冰鲜鸡K值的上升,有利于贮藏期间冰鲜鸡新鲜度的保持,其中,PAW协同有机酸处理抑制效果更佳,PACA与PALA处理组无显著差异(P>0.05),其次是PAW处理组。
脂质氧化是导致肉及肉制品产生异味及食用品质下降的主要因素,并会在贮藏过程中显著加剧[33]。从图4可知,四个处理组中,NaClO处理组脂质氧化现象最为严重,从第0 d的0.15 mg/kg上升至第14 d的1.23 mg/kg。PAW处理组的TBARS含量也在增加,但增加幅度较小,从第0 d的0.14 mg/kg上升至第14 d的0.80 mg/kg;PALA、PACA处理组在脂质氧化方面都表现出良好的抑制效果,在整个储藏期间冰鲜鸡样品的TBARS值都处于较低水平,到了贮藏末期,样品的TBARS值分别为0.70、0.68 mg/kg,丙二醛含量显著降低(P<0.05)。有相关研究表明,肉类的TBARS值在0.5~1.0 mg /kg范围内即发生了脂质氧化[34]。若以0.50 mg /kg作为标准,则NaClO、PAW、PALA、PACA处理组分别于第6、8、12、12 d超出限值。Qian等[35]发现等离子体活化乳酸溶液处理鸡腿肉能够抑制丙二醛含量的增长,这与本文研究结果一致。研究表明,等离子体活化溶液能够抑制鸡肉贮藏期间丙二醛含量的增长,可能与溶液中的NO有关。首先,亚硝酸盐具有抗氧化作用。此外,虽然游离铁会促进脂质氧化的发生,但NO可与肌红蛋白的Fe2+结合,从而减少游离铁的释放来抑制脂质氧化[36–37]。综上可知,PAW、PALA、PACA处理能够延缓冰鲜鸡脂质氧化。
由于亚硝酸盐在一定条件下可能与肉中的蛋白质反应生成致癌物质且过量摄入亚硝酸盐会引发中毒,因此,有必要检测样品的亚硝酸盐含量。从图5可知,冰鲜鸡经等离子体活化溶液处理后,其表面亚硝酸盐含量显著增加(P<0.05),但仍低于检出限值,并不会引发食品安全问题[38]。在贮藏期间,NaClO处理组冰鲜鸡样品的亚硝酸盐含量逐渐增加,从3.69 mg/kg增加至4.67 mg/kg。随着微生物的生长繁殖,硝酸根还原菌及还原酶可将硝酸盐转化为亚硝酸盐[39],从而导致亚硝酸盐含量增加。而等离子体活化溶液处理组样品的亚硝酸盐含量逐渐降低,PAW、PALA、PACA处理组样品的亚硝酸盐含量分别从18.50、19.63、21.43 mg/kg分别降至5.92、7.94、8.04 mg/kg。由于PAW及PAW协同有机酸溶液中含有大量硝酸盐、亚硝酸盐[40],经处理后,冰鲜鸡表面难免会有残留,故而造成冰鲜鸡亚硝酸盐含量显著增加(P<0.05)。在亚硝酸盐还原酶的作用下,亚硝酸盐会被还原为NO,从而导致亚硝酸盐含量降低[41]。
肌肉嫩度是影响鸡肉品质重要因素之一,影响着消费者的偏好和接受程度,可以通过剪切力的大小来评估。从表3可知,随着贮藏时间的延长,不同处理组冰鲜鸡样品的剪切力值呈下降趋势,即冰鲜鸡肉质越嫩。鸡肉在贮藏期间,随着内源性蛋白酶的激活,肌原纤维逐渐降解,肌肉结构松弛,且随着微生物的生长繁殖,肌原纤维降解加剧,从而导致剪切力下降[42]。而PACA、PALA能显著抑制微生物生长繁殖、延缓蛋白质组织的降解,从而能较好地维持鸡肉的嫩度。与NaClO处理组相比,鸡肉经PAW、PALA、PACA处理后,剪切力略有下降,但无显著差异(P>0.05)。研究表明,低浓度的有机酸处理会降低肉品的机械抵抗力并弱化结缔组织,但未对肌肉肌理造成改变,故而PAW协同有机酸处理组样品的剪切力无显著变化[43–44]。此外,还与溶液中的ROS有关。Wang等[45]研究发现,PAW中的ROS能促使氧化应激反应的发生,诱导细胞凋亡,从而改善肉品的嫩度。由此可见,等离子体活化水协同有机酸处理能在一定程度上改善肉类的嫩度。
由表4可知,不同处理组冰鲜鸡样品在色泽、气味、表面组织状态、整体接受性这四个方面的评分随着贮藏时间的延长呈现降低趋势。在整个贮藏期间,NaClO组感官评分下降最快,PAW组下降速度次之,PAW协同有机酸处理组下降速度最慢。在贮藏初期(0~2 d),不同组别感官评分无显著差异(P>0.05)。NaClO组样品在第4 d开始出现表面色泽变淡、鸡肉味淡且伴有些许异味现象但仍处于可接受范围内;到了第6 d出现异味明显、接触样品表面粘手等现象,处于不可接受状态;其余处理组在第6 d仍处于可接受水平。PAW处理组于第10 d达到不可接受状态,PAW协同有机酸处理组在第14 d达到不可接受状态。由于微生物代谢、脂质氧化和降解、蛋白质氧化降解等反应导致鸡肉挥发性成分发生变化,从而导致鸡肉气味发生改变[46],这表明NaClO组很可能在第4 d就已经开始轻微腐败,而PAW、PAW协同有机酸处理具有一定的抑菌效果,故而气味评分降速缓慢。整个贮藏期间,PAW协同有机酸处理组各个方面的评分最高,表明PAW协同有机酸处理能够较好地维持冰鲜鸡的贮藏稳定性。
本文以冰鲜鸡为研究对象,探究了PAW协同有机酸对冰鲜鸡抑菌效果及贮藏品质的影响。研究结果表明,相较于NaClO组,PAW协同有机酸具有显著抑菌效果(P<0.05)。PAW协同有机酸处理在延缓pH上升、维持冰鲜鸡新鲜度以及抑制脂质氧化方面相比NaClO和PAW组具有显著提升(P<0.05)。钱婧[26]的研究也表明,PAW协同有机酸处理可显著抑制鸡肉pH上升并延缓脂质氧化的发生。虽然PAW协同有机酸处理在贮藏前期未对肉色造成不良影响,但到了贮藏末期(12~14 d),鸡肉的a*值降为负数。此外,PAW协同有机酸处理能够较好地维持冰鲜鸡的贮藏稳定性,并在一定程度上能够降低鸡肉的剪切力,改善鸡肉嫩度。综合各项指标,PAW、PALA、PACA处理组的货架期分别从6 d延长至8、12、12 d。本研究结果可为延长肉品货架期及维持贮藏品质提供新的研究思路,为推动PAW在食品领域的应用提供一定的理论参考。然而,目前PAW协同有机酸在肉制品中的应用鲜有报道,该技术的安全性仍待考察,实际应用仍面临挑战;同时PAW协同有机酸的抑菌机理尚不明确,仍待进一步研究。综上所述,PAW在食品领域中具有较高的研究价值,更多的应用领域还有待进一步开发和研究。

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2026年第47卷第12期
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doi: 10.13386/j.issn1002-0306.2025060087
  • 接收时间:2025-06-09
  • 首发时间:2026-10-22
  • 出版时间:2026-06-15
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  • 收稿日期:2025-06-09
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    1岭南现代农业科学与技术广东省实验室云浮分中心,广东云浮 527400
    2南京农业大学食品科技学院,国家肉品质量安全控制工程技术研究中心,江苏南京 210095
    3温氏食品集团股份有限公司,广东云浮 527400

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韩敏义(1975−),男,博士,研究员,研究方向:肉品加工与质量控制,E-mail:。
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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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