Article(id=1320882380273988272, tenantId=1146029695717560320, journalId=1260987677001138203, issueId=1320882022160126288, articleNumber=null, orderNo=null, doi=10.13386/j.issn1002-0306.2024120037, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1733241600000, receivedDateStr=2024-12-04, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1792852500264, onlineDateStr=2026-10-24, pubDate=1767196800000, pubDateStr=2026-01-01, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1792852500264, onlineIssueDateStr=2026-10-24, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1792852500264, creator=13701087609, updateTime=1792852500264, updator=13701087609, issue=Issue{id=1320882022160126288, tenantId=1146029695717560320, journalId=1260987677001138203, year='2026', volume='47', issue='1', pageStart='1', pageEnd='498', issueExtLink='null', onlineDate='null', pubDate='1767196800000', pubDateStr='2026-01-01', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1792852414884, creator='13701087609', updateTime=1792852414884, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=63, endPage=71, ext={EN=ArticleExt(id=1320882380555006641, articleId=1320882380273988272, tenantId=1146029695717560320, journalId=1260987677001138203, language=EN, title=Effect of Steaming Time on Structural Characteristics of Pork Steamed Stuffed Bun Wrapper with Different Gluten Content, columnId=1261336277291548795, journalTitle=Science and Technology of Food Industry, columnName=Research and Investigation, runingTitle=null, highlight=null, articleAbstract=

The industrial production of steamed stuffed bun frequently encounters challenges such as dry wrapper and foaming after steaming. To further investigate the influence of the steaming process on the structural characteristics of steamed stuffed bun, this study utilized flour with protein contents of 8%, 10%, and 12% as raw materials. A combination of experimental techniques, including texture analysis, low-field nuclear magnetic resonance (LF-NMR), Fourier transform infrared spectroscopy (FTIR), fluorescence spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), was employed to analyze the structural characteristics, water distribution, secondary structure, tertiary structure, and microstructure of steamed stuffed bun wrapper at various steaming time points. The results demonstrated that the specific volume, elasticity, and resilience of the steamed stuffed bun wrapper increased with both the extension of steaming time and the increase in protein content. Additionally, the levels of bound water and free water rose, enhancing water fluidity. With the rise in disulfide bond content, the disulfide bonds transitioned from t-g-t and g-g-t structures to g-g-g structures. When the protein content of the steamed stuffed bun wrapper reached 12%, the β-sheet and β-turn structures increased by 4.58% and 8.36%, respectively, as steaming time progressed. At a steaming time of 16 minutes, the β-sheet and β-turn structures increased by 0.31% and 5.06%, respectively, with increasing protein content. The tertiary structure of gluten proteins became more compact as steaming time increased. Simultaneously, non-covalent interactions contributed to the formation of a starch-protein network, with non-covalent bonding forces strengthening as steaming time extended. The crystallinity of starch particles decreased, along with a reduction in the intensity of diffraction peaks. As cooking time increased, starch particles diminished in size and became increasingly embedded within the damaged gluten matrix, forming a starch-gluten protein network system. Furthermore, higher gluten proteins content resulted in tighter integration between starch particles and gluten proteins. These findings provide a theoretical basis for addressing issues related to the hardness and foaming of steamed stuffed bun and offer data support for optimizing process parameters and industrial production.

, authors=Yuchang NIE, Siyu ZHANG, Huining LI, Xuehui CAO, Danshi ZHU, Lina YANG*, authorsList=Yuchang NIE, Siyu ZHANG, Huining LI, Xuehui CAO, Danshi ZHU, Lina YANG, authorCompany=null, correspAuthors=Lina YANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=Copyright © 2026 Science and Technology of Food Industry. All rights reserved., copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1320882383574905532, articleId=1320882380273988272, tenantId=1146029695717560320, journalId=1260987677001138203, language=CN, title=蒸制时间对不同面筋蛋白含量猪肉包子皮结构特性的影响, columnId=1261336277849391232, journalTitle=食品工业科技, columnName=研究与探讨, runingTitle=null, highlight=null, articleAbstract=

包子工业化生产过程常面临着汽蒸制熟后面皮干硬、起泡等问题,为了深入了解蒸制过程对包子皮结构特性的影响,本文以8%、10%、12%蛋白含量的面粉为原料,通过质构、低场核磁共振、傅里叶红外光谱、荧光光谱、X射线衍射、电子扫描显微镜等实验方法,分析其在不同蒸制时间点下包子皮的质构特性、水分分布、二级结构、三级结构及微观结构等结构特性。结果表明:随着蒸制时间和蛋白含量的增加,包子皮的比容、弹性、咀嚼度增加;包子皮结合水和自由水含量增加并且水流动性增强;二硫键增加,二硫键发生了由反-扭-反(t-g-t)结构和扭-扭-反(g-g-t)结构向扭-扭-扭(g-g-g)结构转变的变化;包子皮蛋白含量12%时,随着蒸制时间的增加,β-折叠和β-转角分别增加4.58%和8.36%;蒸制时间为16 min时,随着蛋白含量的增加,β-折叠和β-转角分别增加0.31%和5.06%;面筋蛋白三级结构随蒸制时间增加变得更紧密;同时非共价相互作用参与了淀粉-蛋白质网络的形成,并且随着蒸制时间的增加各非共价键作用力增强;淀粉颗粒结晶度降低,衍射峰强度降低;随着蒸制时间的增加,淀粉颗粒变得越来越小,并紧密地嵌入在被破坏的面筋基质中,形成了淀粉-面筋蛋白网络体系;而且,面筋蛋白含量越大,淀粉颗粒与面筋蛋白结合越紧密。为解决包子皮干硬、起泡奠定了理论基础,为优化包子工艺参数和工业化生产提供数据支撑。

, authors=聂宇嫦, 张司雨, 李慧凝, 曹雪慧, 朱丹实, 杨立娜*, authorsList=聂宇嫦, 张司雨, 李慧凝, 曹雪慧, 朱丹实, 杨立娜, authorCompany=null, correspAuthors=杨立娜, authorNote=

聂宇嫦(1999−),女,硕士研究生,研究方向:粮食、油脂及植物蛋白工程,E-mail:

, correspAuthorsNote=
杨立娜(1987−),女,博士,教授,研究方向:粮油产品精深加工与食品营养,E-mail:。
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聂宇嫦(1999−),女,硕士研究生,研究方向:粮食、油脂及植物蛋白工程,E-mail:

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聂宇嫦(1999−),女,硕士研究生,研究方向:粮食、油脂及植物蛋白工程,E-mail:

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LWT, 2021, 150: 112074., articleTitle=null, refAbstract=null)], funds=null, companyList=[AuthorCompany(id=1320882383813980861, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, xref=null, ext=[AuthorCompanyExt(id=1320882383822369470, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, companyId=1320882383813980861, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=College of Food Science and Engineering, Bohai University, Jinzhou 121013, China), AuthorCompanyExt(id=1320882383830758079, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, companyId=1320882383813980861, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=渤海大学食品科学与工程学院,辽宁锦州 121013)])], figs=[ArticleFig(id=1320882387924398822, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=EN, label=Fig.1, caption=Top view and cross-sectional view of pork buns (a), loss of filling core (b) and quality change of pork steamed stuffed bun wrapper (c), figureFileSmall=hHTCx9WUSeWoyQsz0Igahw==, figureFileBig=iaAF2LG4J2pJCxlbZwM5yg==, tableContent=null), ArticleFig(id=1320882388016673511, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=CN, label=图1, caption=猪肉包俯视图、横截面图(a)、馅心蒸煮损失(b)和包子皮质量变化(c), figureFileSmall=hHTCx9WUSeWoyQsz0Igahw==, figureFileBig=iaAF2LG4J2pJCxlbZwM5yg==, tableContent=null), ArticleFig(id=1320882388289303272, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=EN, label=Fig.2, caption=T2 distribution curve, T2 relaxation time and peak area A2 of pork steamed stuffed bun wrapper, figureFileSmall=H3R3uTuyxewc8zhQ26rmvQ==, figureFileBig=8Wv8ff0I2w9Zq7c+rsUxKQ==, tableContent=null), ArticleFig(id=1320882388377383657, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=CN, label=图2, caption=猪肉包子皮的T2分布曲线、T2弛豫时间和峰面积A2, figureFileSmall=H3R3uTuyxewc8zhQ26rmvQ==, figureFileBig=8Wv8ff0I2w9Zq7c+rsUxKQ==, tableContent=null), ArticleFig(id=1320882388490629866, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=EN, label=Fig.3, caption=FTIR spectra and secondary structure of pork steamed stuffed bun wrapper, figureFileSmall=adTloom4t39gjsVt5nsFew==, figureFileBig=9ET0oR9uUlpRtI3BM0PNvA==, tableContent=null), ArticleFig(id=1320882388549350123, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=CN, label=图3, caption=猪肉包子皮的FTIR光谱和二级结构, figureFileSmall=adTloom4t39gjsVt5nsFew==, figureFileBig=9ET0oR9uUlpRtI3BM0PNvA==, tableContent=null), ArticleFig(id=1320882388608070380, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=EN, label=Fig.4, caption=Endogenous fluorescence and surface hydrophobicity of pork steamed stuffed bun wrapper, figureFileSmall=52JfG3rfplF1TLz0xgXSgQ==, figureFileBig=85s7bILWhtvbvfeVOHoNhA==, tableContent=null), ArticleFig(id=1320882388670984941, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=CN, label=图4, caption=猪肉包子皮的内源荧光和表面疏水性, figureFileSmall=52JfG3rfplF1TLz0xgXSgQ==, figureFileBig=85s7bILWhtvbvfeVOHoNhA==, tableContent=null), ArticleFig(id=1320882388754871022, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=EN, label=Fig.5, caption=XRD and SEM of pork steamed stuffed bun wrapper, figureFileSmall=mY4cmGMVrVIPf7Gdum2qfg==, figureFileBig=EVlAfNmkDMiXQEgG+fUdFQ==, tableContent=null), ArticleFig(id=1320882388893283055, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=CN, label=图5, caption=猪肉包面皮的XRD和SEM图, figureFileSmall=mY4cmGMVrVIPf7Gdum2qfg==, figureFileBig=EVlAfNmkDMiXQEgG+fUdFQ==, tableContent=null), ArticleFig(id=1320882388960391920, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=EN, label=Table 1, caption=

Specific volume and texture characteristics of pork steamed stuffed bun wrapper

, figureFileSmall=null, figureFileBig=null, tableContent=
蛋白含量(%)蒸制时间(min)比容(mL/g)硬度(g)粘附性(N·S)弹性咀嚼度(g)
800.52±0.00Bc246.48±22.55Bd0.43±0.07Bc0.40±0.16Ba42.97±20.00Bd
81.02±0.05Cb987.39±107.95Ac0.78±0.06Ab0.26±0.05ABa205.12±62.85Ac
161.12±0.05Ba1548.78±25.81Ab0.91±0.07Aa0.35±0.01Ba487.02±24.44Ab
241.13±0.03Ca1735.22±69.77Aa0.92±0.03Aa0.44±0.07Ba697.74±69.92Ba
1000.59±0.03Ac316.56±62.80Bc0.67±0.03Ab0.82±0.16Aa175.32±60.42Ab
81.11±0.02Bb526.30±60.13Bb0.34±0.03Bc0.31±0.09Ab55.22±9.41Bb
161.17±0.01Ba819.26±67.22Ca0.79±0.05Aa0.83±0.11Aa543.26±121.93Aa
241.20±0.02Ba924.11±32.16Ba0.82±0.06Ba0.90±0.02Aa682.41±63.55Ba
1200.53±0.03Bc476.99±68.44Ac0.35±0.03Bc0.21±0.03Bc35.27±7.71Bc
81.24±0.02Ab887.69±227.00Ab0.32±0.01Bc0.18±0.01Bc50.82±12.95Bc
161.37±0.02Aa1053.46±131.21Bb0.68±0.02Bb0.79±0.07Ab556.30±31.46Ab
241.37±0.03Aa1480.08±305.35Aa0.75±0.02Ba0.97±0.04Aa1067.82±165.23Aa
), ArticleFig(id=1320882389035889393, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=CN, label=表1, caption=

猪肉包子皮的比容和质构特性

, figureFileSmall=null, figureFileBig=null, tableContent=
蛋白含量(%)蒸制时间(min)比容(mL/g)硬度(g)粘附性(N·S)弹性咀嚼度(g)
800.52±0.00Bc246.48±22.55Bd0.43±0.07Bc0.40±0.16Ba42.97±20.00Bd
81.02±0.05Cb987.39±107.95Ac0.78±0.06Ab0.26±0.05ABa205.12±62.85Ac
161.12±0.05Ba1548.78±25.81Ab0.91±0.07Aa0.35±0.01Ba487.02±24.44Ab
241.13±0.03Ca1735.22±69.77Aa0.92±0.03Aa0.44±0.07Ba697.74±69.92Ba
1000.59±0.03Ac316.56±62.80Bc0.67±0.03Ab0.82±0.16Aa175.32±60.42Ab
81.11±0.02Bb526.30±60.13Bb0.34±0.03Bc0.31±0.09Ab55.22±9.41Bb
161.17±0.01Ba819.26±67.22Ca0.79±0.05Aa0.83±0.11Aa543.26±121.93Aa
241.20±0.02Ba924.11±32.16Ba0.82±0.06Ba0.90±0.02Aa682.41±63.55Ba
1200.53±0.03Bc476.99±68.44Ac0.35±0.03Bc0.21±0.03Bc35.27±7.71Bc
81.24±0.02Ab887.69±227.00Ab0.32±0.01Bc0.18±0.01Bc50.82±12.95Bc
161.37±0.02Aa1053.46±131.21Bb0.68±0.02Bb0.79±0.07Ab556.30±31.46Ab
241.37±0.03Aa1480.08±305.35Aa0.75±0.02Ba0.97±0.04Aa1067.82±165.23Aa
), ArticleFig(id=1320882389119775474, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=EN, label=Table 2, caption=

Contents of sulfhydryl group and disulfide bond (500~550 cm−1) in pork steamed stuffed bun wrapper

, figureFileSmall=null, figureFileBig=null, tableContent=
蛋白含量(%)蒸制时间
(min)
游离巯基
(µmol/g)
扭-扭-扭
(%)
扭-扭-反
(%)
反-扭-反
(%)
8013.43±0.08Bc15.98±0.07Ab30.60±0.02ABb53.42±0.05Aa
816.32±0.48Ab25.86±0.11Ab53.22±0.11Aa20.9±0.08Ab
1620.56±0.89Aa50.31±0.05Aa33.72±0.07Ab15.96±0.07Ab
2414.27±1.02Ac58.17±0.02Aa25.86±0.05Ab15.97±0.06Ab
10014.50±0.62Ac18.13±0.05Ac24.36±0.01Bc57.51±0.05Aa
816.02±0.13Ab33.18±0.05Ab50.51±0.03Aa16.31±0.03Ab
1620.95±0.92Aa62.26±0.04Aa22.76±0.01Ac14.98±0.05Abc
2413.67±0.62Ac60.41±0.03Aa32.56±0.06Ab7.03±0.03Ac
12014.37±0.09Ac13.81±0.04Ac34.19±0.08Ab52.00±0.10Aa
816.01±0.12Ab29.51±0.02Ab55.34±0.08Aa15.15±0.06Ab
1621.34±0.33Aa60.28±0.11Aa27.04±0.10Ab12.68±0.06Ab
2413.09±0.05Ad50.48±0.11Aa34.86±0.07Ab14.66±0.08Ab
), ArticleFig(id=1320882389199467251, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=CN, label=表2, caption=

猪肉包子皮的巯基和二硫键(500~550 cm−1)含量

, figureFileSmall=null, figureFileBig=null, tableContent=
蛋白含量(%)蒸制时间
(min)
游离巯基
(µmol/g)
扭-扭-扭
(%)
扭-扭-反
(%)
反-扭-反
(%)
8013.43±0.08Bc15.98±0.07Ab30.60±0.02ABb53.42±0.05Aa
816.32±0.48Ab25.86±0.11Ab53.22±0.11Aa20.9±0.08Ab
1620.56±0.89Aa50.31±0.05Aa33.72±0.07Ab15.96±0.07Ab
2414.27±1.02Ac58.17±0.02Aa25.86±0.05Ab15.97±0.06Ab
10014.50±0.62Ac18.13±0.05Ac24.36±0.01Bc57.51±0.05Aa
816.02±0.13Ab33.18±0.05Ab50.51±0.03Aa16.31±0.03Ab
1620.95±0.92Aa62.26±0.04Aa22.76±0.01Ac14.98±0.05Abc
2413.67±0.62Ac60.41±0.03Aa32.56±0.06Ab7.03±0.03Ac
12014.37±0.09Ac13.81±0.04Ac34.19±0.08Ab52.00±0.10Aa
816.01±0.12Ab29.51±0.02Ab55.34±0.08Aa15.15±0.06Ab
1621.34±0.33Aa60.28±0.11Aa27.04±0.10Ab12.68±0.06Ab
2413.09±0.05Ad50.48±0.11Aa34.86±0.07Ab14.66±0.08Ab
), ArticleFig(id=1320882389283353332, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=EN, label=Table 3, caption=

Starch and protein content in pork steamed stuffed bun wrapper under different treatment methods (%)

, figureFileSmall=null, figureFileBig=null, tableContent=
蛋白含量(%)样品蒸制时间(min)CtrlNaClUreaSDS
8%沉淀蛋白06.81±0.18Cc6.17±0.18Ca5.32±0.18Ca5.43±0.32Ca
87.24±0.18Cb5.64±0.18Cb4.89±0.19Ca5.32±0.18Ca
167.77±0.18Ca5.53±0.18Cbc3.62±0.18Cb4.26±0.49Cb
247.87±0.18Ca5.21±0.18Cc3.51±0.32Bb3.73±0.18Cb
10%08.62±0.00Bd9.15±0.18Ba6.92±0.18Ba7.55±0.18Ba
88.94±0.00Bc8.73±0.18Bab5.85±0.49Bb6.92±0.18Bb
169.26±0.00Bb8.41±0.18Bb4.89±0.18Bc6.49±0.18Bc
249.79±0.18Ba7.77±0.37Bc3.30±0.18Bd6.17±0.18Bc
12%010.11±0.18Ac10.53±0.32Aa8.30±0.32Aa9.15±0.18Aa
810.43±0.18Ac10.00±0.18Aa7.45±0.18Ab8.72±0.18Ab
1611.38±0.18Ab10.53±0.32Aa6.17±0.18Ac8.19±0.18Ac
2411.92±0.18Aa10.11±0.18Aa5.53±0.18Ad7.77±0.18Ad
8%沉淀淀粉063.89±0.69Ad84.11±1.44Aa55.19±0.12Ca62.32±0.07Aa
865.39±0.65Ac78.85±0.72Ab54.86±0.19Ab61.37±0.72Aa
1670.61±0.12Ab77.65±0.07Ab52.37±0.07Ac59.83±0.68Ab
2474.75±0.50Aa73.13±0.07Ac51.67±0.07Ad59.04±0.00Ab
10%062.40±0.12Bd70.27±0.07Ba55.98±0.07Aa60.04±0.12Ca
865.34±0.07Ac65.47±0.07Bb52.08±0.00Bb53.91±0.59Cb
1670.07±0.72Ab60.04±0.65Bc49.60±0.65Bc52.91±0.26Cb
2471.19±0.07Ba57.55±0.00Bd47.78±0.61Bd50.88±0.80Bc
12%061.53±0.00Cd68.87±0.57Ba55.77±0.07Ba60.54±0.22Ba
862.32±0.07Bc63.81±0.07Cb51.84±0.12Bb55.52±0.40Bb
1669.03±0.14Bb59.21±0.07Cc49.97±0.00Bc54.16±0.07Bc
2470.98±0.12Ba56.15±0.07Cd48.19±0.79Bd51.46±0.00Bd
), ArticleFig(id=1320882389379822325, tenantId=1146029695717560320, journalId=1260987677001138203, articleId=1320882380273988272, language=CN, label=表3, caption=

不同处理方法下猪肉包子皮的淀粉和蛋白质含量(%)

, figureFileSmall=null, figureFileBig=null, tableContent=
蛋白含量(%)样品蒸制时间(min)CtrlNaClUreaSDS
8%沉淀蛋白06.81±0.18Cc6.17±0.18Ca5.32±0.18Ca5.43±0.32Ca
87.24±0.18Cb5.64±0.18Cb4.89±0.19Ca5.32±0.18Ca
167.77±0.18Ca5.53±0.18Cbc3.62±0.18Cb4.26±0.49Cb
247.87±0.18Ca5.21±0.18Cc3.51±0.32Bb3.73±0.18Cb
10%08.62±0.00Bd9.15±0.18Ba6.92±0.18Ba7.55±0.18Ba
88.94±0.00Bc8.73±0.18Bab5.85±0.49Bb6.92±0.18Bb
169.26±0.00Bb8.41±0.18Bb4.89±0.18Bc6.49±0.18Bc
249.79±0.18Ba7.77±0.37Bc3.30±0.18Bd6.17±0.18Bc
12%010.11±0.18Ac10.53±0.32Aa8.30±0.32Aa9.15±0.18Aa
810.43±0.18Ac10.00±0.18Aa7.45±0.18Ab8.72±0.18Ab
1611.38±0.18Ab10.53±0.32Aa6.17±0.18Ac8.19±0.18Ac
2411.92±0.18Aa10.11±0.18Aa5.53±0.18Ad7.77±0.18Ad
8%沉淀淀粉063.89±0.69Ad84.11±1.44Aa55.19±0.12Ca62.32±0.07Aa
865.39±0.65Ac78.85±0.72Ab54.86±0.19Ab61.37±0.72Aa
1670.61±0.12Ab77.65±0.07Ab52.37±0.07Ac59.83±0.68Ab
2474.75±0.50Aa73.13±0.07Ac51.67±0.07Ad59.04±0.00Ab
10%062.40±0.12Bd70.27±0.07Ba55.98±0.07Aa60.04±0.12Ca
865.34±0.07Ac65.47±0.07Bb52.08±0.00Bb53.91±0.59Cb
1670.07±0.72Ab60.04±0.65Bc49.60±0.65Bc52.91±0.26Cb
2471.19±0.07Ba57.55±0.00Bd47.78±0.61Bd50.88±0.80Bc
12%061.53±0.00Cd68.87±0.57Ba55.77±0.07Ba60.54±0.22Ba
862.32±0.07Bc63.81±0.07Cb51.84±0.12Bb55.52±0.40Bb
1669.03±0.14Bb59.21±0.07Cc49.97±0.00Bc54.16±0.07Bc
2470.98±0.12Ba56.15±0.07Cd48.19±0.79Bd51.46±0.00Bd
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蒸制时间对不同面筋蛋白含量猪肉包子皮结构特性的影响
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聂宇嫦 , 张司雨 , 李慧凝 , 曹雪慧 , 朱丹实 , 杨立娜 *
食品工业科技 | 研究与探讨 2026,47(1): 63-71
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食品工业科技 |研究与探讨 2026 , 47 (1) : 63 -71
蒸制时间对不同面筋蛋白含量猪肉包子皮结构特性的影响
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聂宇嫦 , 张司雨, 李慧凝, 曹雪慧, 朱丹实, 杨立娜*
作者信息
  • 渤海大学食品科学与工程学院,辽宁锦州 121013
通讯作者:
杨立娜(1987−),女,博士,教授,研究方向:粮油产品精深加工与食品营养,E-mail:。
作者简介:

聂宇嫦(1999−),女,硕士研究生,研究方向:粮食、油脂及植物蛋白工程,E-mail:

Effect of Steaming Time on Structural Characteristics of Pork Steamed Stuffed Bun Wrapper with Different Gluten Content
Yuchang NIE , Siyu ZHANG, Huining LI, Xuehui CAO, Danshi ZHU, Lina YANG*
Affiliations
  • College of Food Science and Engineering, Bohai University, Jinzhou 121013, China
出版时间: 2026-01-01 doi: 10.13386/j.issn1002-0306.2024120037
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包子工业化生产过程常面临着汽蒸制熟后面皮干硬、起泡等问题,为了深入了解蒸制过程对包子皮结构特性的影响,本文以8%、10%、12%蛋白含量的面粉为原料,通过质构、低场核磁共振、傅里叶红外光谱、荧光光谱、X射线衍射、电子扫描显微镜等实验方法,分析其在不同蒸制时间点下包子皮的质构特性、水分分布、二级结构、三级结构及微观结构等结构特性。结果表明:随着蒸制时间和蛋白含量的增加,包子皮的比容、弹性、咀嚼度增加;包子皮结合水和自由水含量增加并且水流动性增强;二硫键增加,二硫键发生了由反-扭-反(t-g-t)结构和扭-扭-反(g-g-t)结构向扭-扭-扭(g-g-g)结构转变的变化;包子皮蛋白含量12%时,随着蒸制时间的增加,β-折叠和β-转角分别增加4.58%和8.36%;蒸制时间为16 min时,随着蛋白含量的增加,β-折叠和β-转角分别增加0.31%和5.06%;面筋蛋白三级结构随蒸制时间增加变得更紧密;同时非共价相互作用参与了淀粉-蛋白质网络的形成,并且随着蒸制时间的增加各非共价键作用力增强;淀粉颗粒结晶度降低,衍射峰强度降低;随着蒸制时间的增加,淀粉颗粒变得越来越小,并紧密地嵌入在被破坏的面筋基质中,形成了淀粉-面筋蛋白网络体系;而且,面筋蛋白含量越大,淀粉颗粒与面筋蛋白结合越紧密。为解决包子皮干硬、起泡奠定了理论基础,为优化包子工艺参数和工业化生产提供数据支撑。

汽蒸时间  /  面筋蛋白含量  /  包子皮  /  结构特性

The industrial production of steamed stuffed bun frequently encounters challenges such as dry wrapper and foaming after steaming. To further investigate the influence of the steaming process on the structural characteristics of steamed stuffed bun, this study utilized flour with protein contents of 8%, 10%, and 12% as raw materials. A combination of experimental techniques, including texture analysis, low-field nuclear magnetic resonance (LF-NMR), Fourier transform infrared spectroscopy (FTIR), fluorescence spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), was employed to analyze the structural characteristics, water distribution, secondary structure, tertiary structure, and microstructure of steamed stuffed bun wrapper at various steaming time points. The results demonstrated that the specific volume, elasticity, and resilience of the steamed stuffed bun wrapper increased with both the extension of steaming time and the increase in protein content. Additionally, the levels of bound water and free water rose, enhancing water fluidity. With the rise in disulfide bond content, the disulfide bonds transitioned from t-g-t and g-g-t structures to g-g-g structures. When the protein content of the steamed stuffed bun wrapper reached 12%, the β-sheet and β-turn structures increased by 4.58% and 8.36%, respectively, as steaming time progressed. At a steaming time of 16 minutes, the β-sheet and β-turn structures increased by 0.31% and 5.06%, respectively, with increasing protein content. The tertiary structure of gluten proteins became more compact as steaming time increased. Simultaneously, non-covalent interactions contributed to the formation of a starch-protein network, with non-covalent bonding forces strengthening as steaming time extended. The crystallinity of starch particles decreased, along with a reduction in the intensity of diffraction peaks. As cooking time increased, starch particles diminished in size and became increasingly embedded within the damaged gluten matrix, forming a starch-gluten protein network system. Furthermore, higher gluten proteins content resulted in tighter integration between starch particles and gluten proteins. These findings provide a theoretical basis for addressing issues related to the hardness and foaming of steamed stuffed bun and offer data support for optimizing process parameters and industrial production.

steaming time  /  gluten content  /  steamed stuffed bun wrapper  /  structural characteristic
聂宇嫦, 张司雨, 李慧凝, 曹雪慧, 朱丹实, 杨立娜. 蒸制时间对不同面筋蛋白含量猪肉包子皮结构特性的影响. 食品工业科技, 2026 , 47 (1) : 63 -71 . DOI: 10.13386/j.issn1002-0306.2024120037
Yuchang NIE, Siyu ZHANG, Huining LI, Xuehui CAO, Danshi ZHU, Lina YANG. Effect of Steaming Time on Structural Characteristics of Pork Steamed Stuffed Bun Wrapper with Different Gluten Content[J]. Science and Technology of Food Industry, 2026 , 47 (1) : 63 -71 . DOI: 10.13386/j.issn1002-0306.2024120037
包子是中国传统发酵类主食之一,由于营养性好、易消化、具有较好的质构和感官品质[1],因此备受人们的喜爱。但是包子工业化生产过程常面临着汽蒸制熟后包子皮起泡、泛白、皱缩等问题,严重影响感官和食用品质。
导致包子皮品质劣变的因素很多,如面粉种类、醒发时间等[2]。但是,目前的研究大多集中在面粉的种类和醒发条件优化等方面,对汽蒸过程中包子皮的结构特性变化研究较少。王晓曦等[3]研究发现高筋面团的发酵活力高于低筋面团,所以用高筋粉制作的包子品质好于低筋粉。张剑等[4]研究表明大多数小麦品种的蛋白质和粉质指标对馒头的比容具有显著影响,蛋白质含量显著影响馒头的弹性和黏性,而沉降值则对馒头内部结构的形成具有重要影响。刘长虹等[5]研究发现,当醒发时间为35 min左右、醒发温度在35 ℃左右、醒发湿度在80%左右时馒头的品质最佳。汽蒸是一种传统热加工工艺,制作出的成品质地湿润、柔软,是面筋蛋白网络结构形成的重要过程。而面筋网络结构的形成与持水能力,很大程度上取决于原料中面筋蛋白的含量,进而改变包子皮的感官与食用品质。
因此,本文以不同面筋蛋白含量的小麦粉制作包子皮,探究包子皮在不同蒸制时间点的水分分布和结构特性变化,明确包子皮最佳生产工艺,为提高包子工业化生产质量提供理论依据。
低筋小麦粉、高筋小麦粉 益海嘉里金龙鱼粮油食品股份有限公司;中筋小麦粉 江苏新良面粉有限责任公司;酵母 安琪酵母股份有限公司;猪肉馅 苏州旭方食品科技发展有限公司;总淀粉试剂盒 北京索莱宝科技有限公司。
TA-XT plus质构仪 上海瑞玢智能科技有限公司;MesoMR23-040H-I低场核磁共振分析仪 苏州纽迈分析仪器股份有限公司;IRTracer-100傅里叶变换红外光谱仪 日本岛津仪器有限公司;Ultima IV粉末X-射线衍射仪 日本理学仪器有限公司;Regulus 8100电子显微镜 日本日立仪器有限公司。
猪肉包子的制备按照朱在勤等[6]的方法并稍作调整。在室温下将500 g面粉(蛋白含量8%、10%、12%)、5 g酵母、260 g水倒入和面机搅拌10 min,用压面机在5 mm辊距下压面10次,将面团均分为30 g,用擀面杖将压扁的面团从边缘向内擀制成面皮,再包入制作好的多汁馅心20 g,放入温度37 ℃,相对湿度75%的醒发箱40 min,上锅分别蒸制0、8、16、24 min,3 min后取出包子盖上纱布冷却30 min,用于包子比容测定。剥取包子皮用于质构和水分分布的测定。将包子皮冻干、磨粉和过100目筛,得到猪肉包子皮冻干粉用于游离巯基、拉曼光谱、FT-IR、荧光特性、表面疏水性、分子间作用力、XRD、微观结构的测定。
将冷却30 min的猪肉包子整个称重,并通过菜籽置换法测量包子体积[7]。
$ \mathrm{包}\mathrm{子}\mathrm{比}\mathrm{容}\left(\mathrm{m}\mathrm{L}/\mathrm{g}\right)=体积/质量 $
参考O'Neill等[8]的方法并稍作修改,记录猪肉包子馅心蒸制前后的重量,计算猪肉包馅心蒸煮损失。计算公式:
$\begin{split}&\mathrm{馅}\mathrm{心}\mathrm{蒸}\mathrm{煮}\mathrm{损}\mathrm{失}(\text{%})=\\&(\mathrm{蒸}\mathrm{制}\mathrm{前}\mathrm{的}\mathrm{重}\mathrm{量}-\mathrm{蒸}\mathrm{制}\mathrm{后}\mathrm{的}\mathrm{重}\mathrm{量})\times 100/\mathrm{蒸}\mathrm{制}\mathrm{前}\mathrm{的}\mathrm{重}\mathrm{量}\end{split}$
参考O'Neill等[8]的方法并稍作修改,用电子秤记录包子皮蒸制0、8、16、24 min时重量,得出猪肉包子皮质量变化。
参考Curti等[9]的方法并稍作修改,将冷却后的猪肉包子用镊子去除外表皮和馅心,将包子皮切割成1 cm×1 cm×1 cm立方体,用圆柱形探头(P/50)进行质构测试。测试参数:距离30 mm,测试前速率3 mm/s,测试速率1 mm/s,测试后速率1 mm/s,下压程度60%,测试力0.08 N,2次压缩时间间隔3 s,测定包子皮的硬度、粘附性、弹性、咀嚼度,每个样品重复测量三次。
参考Yang等[10]的方法并稍作修改,将冷却后的猪肉包子用镊子去除外表皮和馅心,将包子皮切割成1 cm×1 cm×1 cm立方体,置于25 mm的线圈中进行测试,工作频率为1 Hz,共振频率为21 MHz。采用CPMG脉冲序列检测弛豫时间T2。测试参数:射频延时0.04 ms,前放档位2档,等待时间500 ms,累加次数4次,回波个数18000,回波时间0.2 ms。
150 mg猪肉包子皮冻干粉加入1 mL Tris-Gly缓冲液(pH8.0),涡旋混匀,再加入4.7 g盐酸胍,Tris-Gly缓冲液定容至10 mL,涡旋混匀。取1 mL加入4 mL溶液(8 mol/L脲+5 mol/L盐酸胍)和0.1 mL Ellman’s试剂,涡旋混匀后于412 nm测吸光值A,计算巯基含量[11]。
$ -\mathrm{S}\mathrm{H}({\text{μ}}{{\mathrm{m}}}\mathrm{o}\mathrm{l}/\mathrm{g})=73.53{\mathrm{A}}_{412}{\mathrm{D}}/{\mathrm{C}} $
式中,A412为412 nm处的吸光值,D为稀释因子5.02,C为样品浓度(mg(干物)/mL),本实验为15 mg/mL,比色皿光程为10 mm。
拉曼光谱的检测参考Ørskov等[12]的方法并稍作修改。将猪肉包子皮冻干粉置于载玻片上,然后用激光光源进行激光聚焦。激光测试波长为633 nm,功率为10 mW,扫描范围为400~600 cm−1,分辨率为2 cm−1,数据采集时间为5 s,扫描次数为3次。
采用FT-IR测定包子皮的红外光谱特性[13]。将猪肉包子皮冻干粉和KBr按1:100的比例研磨。扫描波数范围4000~500 cm−1,扫描64次,分辨率4 cm−1。
将150 mg猪肉包子皮冻干粉溶于30 mL 0.1 mol/L PBS缓冲液(pH7.0)中,4000 r/min离心20 min,取上清液测定荧光光谱[14]。测试参数:激发波长260 nm,发射起始波长270 nm,发射终止波长450 nm,激发狭缝宽度2.5 nm,发射狭缝宽度5.0 nm。
将10 mg猪肉包子皮冻干粉溶于1 mL 0.02 mol/L PBS缓冲液(pH7.0)中,加入200 μL 1 mg/mL溴酚蓝指示剂。室温下摇床振荡10 min,4000 r/min离心10 min。取上清,稀释10倍,加入96孔板,每孔200 µL,酶标仪595 nm处测定其吸光度A[15],以不加样品的试剂作为空白对照,溴酚蓝的结合量与表面疏水性成正比。
$ \mathrm{溴}\mathrm{酚}\mathrm{蓝}\mathrm{结}\mathrm{合}\mathrm{量}\left(\text{μ}\mathrm{g}\right)=200\times \left({\mathrm{A}}_{\mathrm{空}}-{\mathrm{A}}_{\mathrm{样}}\right)/{\mathrm{A}}_{\mathrm{空}} $
参考Li等[16]的方法并略作修改,称取2.5 g包子皮冻干粉,将其与去离子水1:3混合得到面糊,将面糊分别置于30 mL浓度为2%(w/w)的NaCl、SDS、尿素试剂中,涡旋5 s混匀,得到浓度25%(w/w)的溶液。在25 ℃、200 r/min下振荡4 h后,4000 r/min离心5 min,弃去上清液,用去离子水分3次洗沉淀,每次洗沉淀都以4000 r/min离心5 min,将沉淀物在40 ℃下烘箱干燥24 h,过100目筛网,采用总淀粉测定试剂盒测定淀粉含量,参考GB 5009.5-2016《食品中蛋白质的测定》中凯氏定氮法测定蛋白质含量。沉淀中的淀粉和蛋白含量与分子间相互作用力成反比。
将包子皮冻干粉过100目筛网,取适量粉末置于XRD衍射仪上,工作电流为27 mA,电压为50 kV。扫描速度为4°/min,衍射角θ的扫描范围为5°~50°[17]。
将包子皮冻干粉固定在观察台,真空下喷金,15 kV加速电压下,通过电子扫描显微镜放大1500倍观察其微观结构。
所有数据均进行3次重复,以$ \bar{\mathrm{x}}\pm \mathrm{s} $表示,应用SPSS 23.0软件对数据进行单因素方差分析,以P<0.05为差异显著,Origin 2023对数据作图处理,通过OMNIC和PeakFit软件进行数据分析。
如表1所示,随着蒸制时间的增加,包子皮的比容、硬度、粘附性、弹性和咀嚼度均显著增加(P<0.05),并在16 min后趋于稳定,均未出现收缩现象。在蒸制时,包子皮内部温度不断升高,无定形的面筋蛋白网络逐渐形成蛋白凝胶网络结构,硬度增加[18];淀粉颗粒糊化,粘附性增加[19];蒸制前酵母菌快速生长繁殖,产生大量CO2,包子皮形成气孔,随着蒸制时间的增加,气孔体积增大,使包子比容增大,包子皮富有弹性[20]。同一蒸制时间时,随着蛋白含量的升高,面皮的硬度先降低后升高,粘附性降低,而比容、弹性、咀嚼度逐渐增加。8%蛋白含量的包子皮硬度和粘附性高,弹性和咀嚼度低,可能因为面筋蛋白含量低、结构松散、猪肉馅心质量减少,汤汁和水蒸气易渗入包子皮(如图1b、c所示),使得其面筋网络结构稳定性变差,持气能力降低,硬度增加。12%蛋白含量的包子皮硬度和粘附性适中,弹性和咀嚼度高,可能因为面筋蛋白含量高、网络结构稳定、水分逸出缓慢,赋予包子皮丰富的弹性结构,更耐咀嚼。因此,12%蛋白含量蒸制16 min有助于提高猪肉包子皮的质构特性。
T2代表水的迁移能力,T2值越小,这部分水的流动性越弱,与其他组分的结合越紧密。猪肉包子皮T2主要有三个峰,T21峰(0.1~1 ms)视为强结合水,是以共价键形式与面筋蛋白和淀粉结合的水,流动性弱;T22峰(6~20 ms)视为弱结合水,是存在于蛋白和淀粉分子间的水,流动性较强;T23峰(60~200 ms)视为自由水,流动性更强[21]。如图2所示,蒸制8 min时,各组包子皮均呈现T21、T22显著增加和T23显著降低(P<0.05)。可能是由于加热导致水的运动加剧,面筋蛋白、淀粉的强结合水和弱结合水流动性增强;蒸制8~24 min时,各组包子皮面筋蛋白网络体系逐渐形成,淀粉糊化,水分流动性减弱。T21和T22组分的面积占比大于90%,说明水分主要以结合水状态存在。随蒸制时间增加,A21没有明显变化,A22增加最为明显,A23缓慢增加,可能是因为淀粉胀润和面筋的形成过程吸收水分,而且蒸制过程一部分水汽和馅心汤汁进入面团内部存在于淀粉-蛋白凝胶框架的内部空间,提高了弱结合水和自由水含量[22]。不同蛋白含量的面皮呈现相同的水分变化趋势。
二硫键的形成与游离巯基含量呈负相关,影响淀粉-面筋蛋白网络结构的稳定[23]。如表2所示,蒸制时间从0 min到16 min,包子皮中游离巯基呈现增加趋势,包子皮中的面筋蛋白发生变性从而展开,游离巯基含量增加[24]。蒸制时间为24 min时,游离巯基含量下降,随着温度的上升,包子皮中的游离巯基发生氧化还原反应生成了二硫键[25]。包子皮蛋白含量对游离巯基含量基本未见影响。
在拉曼光谱中波段为500~550 cm−1的特征峰是二硫键的特征谱带,其中510 cm−1附近为扭-扭-扭(g-g-g)结构、527 cm−1附近为扭-扭-反(g-g-t)结构、546 cm−1附近为反-扭-反(t-g-t)结构,其中g-g-g结构最稳定[26]。二硫键结构的稳定性与吸收频率有关,随着吸收频率的增加,二硫键稳定性降低[27]。如表2所示,随着蒸制时间的增加,二硫键中g-g-g结构含量增加,t-g-t结构降低,包子皮中的二硫键由t-g-t结构和g-g-t结构向g-g-g结构转变,二硫键的稳定性增加。因此,包子皮中淀粉-面筋蛋白的网络结构增强。不同蛋白含量的包子皮呈现相同的二硫键变化趋势。
图3为不同蒸制时间下猪肉包子皮的FTIR光谱,各组包子皮在3600~3000 cm−1处均有一个强且宽的吸收峰,是-OH的特征,随着蒸制时间的增加,此吸收峰强度逐渐变弱,可能是由于淀粉分子的O-H基团发生了交联缩合[28]。
1600~1700 cm−1是酰胺I带吸收峰的范围,反映蛋白的二级结构[29]。α-螺旋特征峰出现在1646~1664 cm−1,与面团弹性呈负相关;β-折叠特征峰集中在1600~1637 cm−1和1682~1700 cm−1,影响面筋蛋白的稳定性;β-转角特征峰集中在1664~1682 cm−1,与面团的延展性和黏性有关;而无规卷曲特征峰出现在1637~1645 cm−1,与面筋蛋白的不稳定性相关[30]。随着蒸制时间的增加,α-螺旋和无规则卷曲减小,β-折叠和β-转角含量增加,这可能是面筋蛋白分子链在加热过程中展开,发生了二硫键、氢键和疏水相互作用的重排[29],使包子皮弹性和黏性增加。
不同面筋蛋白含量的面粉制作而成的包子皮蛋白的二级结构也不同,随着蛋白含量的增加,α-螺旋相对含量降低,β-折叠和β-转角相对含量增多,无规则卷曲相对含量无明显变化。说明面筋蛋白聚集程度越高,形成的面筋网络结构越致密,面皮的筋力越强,面筋结构的稳定性越强,与淀粉结合程度越高。
色氨酸常作为内源荧光探针来检测蛋白质三级结构变化[31]。由图4可知,蒸制16 min,包子皮中面筋蛋白的荧光光谱发生明显红移,蒸制16~24 min,荧光光谱红移减弱,荧光强度随着蒸制时间的延长而降低。在加热过程中色氨酸残基暴露增多,随后色氨酸可能与其他侧链氨基酸发生聚集和交联,促进面筋蛋白聚集,三级结构逐渐紧密,进而导致荧光强度降低[32]。荧光强度随着包子皮中蛋白含量的增加而增加,但不同蛋白含量的包子皮荧光光谱峰没有发生位移。
蛋白表面疏水性反映了蛋白分子表面疏水基团的数量和蛋白三级结构的变化[32]。由图4d可以看出包子皮表面疏水性随蒸制时间的增大呈现先增加后降低的趋势,蒸制8 min时,表面疏水性最高,面筋蛋白分子链展开,疏水区暴露[33];同时色氨酸残基暴露于亲水环境,迫使疏水基团相互结合[34],面筋蛋白聚集,包子皮表面疏水性降低。蛋白含量从8%增加到12%,包子皮的表面疏水性增大,面皮结构稳定性增强。
猪肉包子皮的三级结构、交联聚集程度受淀粉与面筋蛋白的分子间非共价键影响,主要包括氢键、静电作用、疏水作用等[35]。尿素会破坏氢键,NaCl会影响静电作用,SDS会影响疏水性[16]。如表3所示,随着蒸制时间的增加,未添加试剂的包子皮蛋白和淀粉含量增加,可能是因为蒸制后的淀粉-面筋蛋白分子间作用力增强,溶解度降低;尿素和SDS的添加导致包子皮中蛋白质和淀粉含量降低,并且随着蒸制时间的增加降低越多,可能是因为尿素和SDS的添加分别破坏了氢键和疏水性[36],从而促进蛋白和淀粉浸出到溶液中,导致沉淀物中二者含量降低;在蒸制初期,添加NaCl后包子皮的蛋白质和淀粉含量高于未添加试剂的包子皮(除蛋白含量8%组),可能是因为蛋白质中可电离的氨基酸残基较少,离子间相互作用较弱[37]。因此,非共价键参与了淀粉-蛋白网络的形成。而且,面皮的蛋白含量从8%增加到12%,会导致沉淀样品中蛋白含量增加,淀粉含量减少。
包子皮弹性和结晶度呈负相关[38]。如图5所示,0 min时包子皮在15°、18°、20°和23°处出现峰,呈a型晶体结构[21],随着蒸制时间的增加,15°、18°和23°处的衍射峰消失,可能是因为淀粉颗粒在蒸制过程中糊化。在蒸制0~8 min时,淀粉颗粒呈a型晶体结构保持不变。蒸制8 min之后,包子受热逐渐增加,15°、18°和23°处衍射峰消失,淀粉颗粒结晶度降低,衍射峰强度降低[39]。
猪肉包子皮的微观结构如图5所示,蒸制0 min时,淀粉颗粒呈现球状并且被面筋蛋白包裹;蒸制8 min时,部分淀粉颗粒糊化,蛋白分子变性,淀粉-蛋白网络体系初步形成;随着蒸制时间增加至16 min,蛋白分子进一步变性,淀粉糊化完全,并紧密地嵌入被破坏的面筋基质中,形成淀粉-面筋蛋白网络体系;蒸制24 min时,包子皮的形貌没有明显的变化。蛋白含量越大,淀粉颗粒与面筋蛋白结合得越紧密。
随着蛋白含量和蒸制时间的增加,包子皮的结构特性和品质得到改善,其中蛋白含量为12%的包子蒸制16 min时,包子皮的质构特性最佳。随着蒸制时间和蛋白含量的增加,包子皮的比容、弹性增加,包子皮的品质提高。蒸制过程为包子皮提供了能量和水蒸气。同时,随着蒸制时间的增加,蒸制温度上升,面筋蛋白发生变性从而展开,游离巯基含量增多,促进了二硫键的生成。随着蒸制时间的增加,β-折叠和β-转角含量增加,面皮面筋蛋白结构稳定性增强;包子皮三级结构随蒸制时间增加而逐渐紧密。同时非共价键参与了淀粉-蛋白质网络的形成,并且随着蒸制时间的增加各非共价键作用力增强,提高了淀粉-面筋蛋白结构的稳定性,进而提高了包子皮的品质。

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2026年第47卷第1期
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doi: 10.13386/j.issn1002-0306.2024120037
  • 接收时间:2024-12-04
  • 首发时间:2026-10-24
  • 出版时间:2026-01-01
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  • 收稿日期:2024-12-04
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    渤海大学食品科学与工程学院,辽宁锦州 121013

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杨立娜(1987−),女,博士,教授,研究方向:粮油产品精深加工与食品营养,E-mail:。
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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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