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Outline
To optimize the fresh juice extraction process of Premna microphylla and evaluate its antioxidant and enzyme inhibitory activities.
Used fresh Premna microphylla leaves as raw material, the extraction conditions were optimized through single-factor tests and response surface methodology. The free radical scavenging ability and total antioxidant capacity of the obtained freshly squeezed juice were determined. An in vitro enzyme inhibition system was established using α-amylase and α-glucosidase to determine the inhibitory effects of the juice on these enzymes.
The optimal extraction conditions were blanching temperature 61 °C, blanching time 56 s and leaf-to-water ratio 1:3 (m:V). Under these conditions, the content of total flavonoids, total protein and pectin in the juice were (15.030±0.039) mg/g, (78.000±0.032) mg/mL and (2.070±0.007) μmol/g, respectively. The half maximal inhibitory concentration values for scavenging 1,1-diphenyl-2-picrylhydrazyl and 2,2’-biazo-bis-3-ethylbenzothiazolin-6-sulfonic acid radical scavenging were 270.70 mg/mL and 219.30 mg/mL, respectively, while the total antioxidant capacity reached 0.572 mmol/g. The inhibition rates on α-amylase and α-glucosidase activities were 4.09% and 5.01%, respectively.
The optimized process significantly enhance the content of active compounds in Premna microphylla fresh juice, demonstrating excellent antioxidant and hypoglycemic potential, providing a foundation for the development of Premna microphylla specialty foods and its use as a food additive.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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