Full
Outline
To compare the pretreatment methods for the determination of aflatoxin in tea by ultra performance liquid chromatography-tandem mass spectrometry.
The 3 kinds of different pretreatment methods, immunoaffinity column method, QuEChERS and multifunctional column were adopted to pretreat the tea, the aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1) and aflatoxin G2 (AFG2) in tea were detected by ultra performance liquid chromatography-tandem mass spectrometry, and the effects of 3 kinds of different pretreatment methods on the determination results were compared. The purification effect, matrix effect, stability, repeatability, limits of detection and recovery rates of the 3 kinds of pretreatment methods were compared.
The immunoaffinity column method had low matrix effects and less impurities. The 3 kinds of different pretreatment methods exhibited a good linear relationship for aflatoxin in the concentration range of 1–60 μg/L, the correlation coefficients were not less than 0.999. The average recoveries ranged from 78.6%–107.1% with relative standard deviations of 1.3%–3.1%. The limits of detection ranged from 0.04–0.22 μg/kg. There was no significant difference in the limits of detection between immunoaffinity column method and multifunctional column method, but the results of limit of detection showed that immunoaffinity column method and multifunctional column method were better than QuEChERS method. The results obtained from the quality control samples of tea matrix after 3 kinds of pretreatment methods were used, the difference was within the acceptable range. The 3 kinds of methods could meet the experimental requirements in the detection of AFB1, AFB2, AFG1, AFG2 in tea matrix.
These 3 kinds of methods are all suitable for the determination of AFB1, AFB2, AFG1, AFG2 in tea. The immunoaffinity column method has low matrix effects and high sensitivity, and therefore can be used to determine low concentration samples. QuEChERS method is better in terms of simple operation and instrument maintenance cost saving than multifunctional column. QuEChERS method is simple and efficient, and it is suitable for large-scale samples detection.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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