Full
Outline
To establish an automated and rapid method for the detection of clothianidin, dimethomorph and carbendazim residues in vegetables based on magnetic solid-phase extraction technology and a microarray biochip kit.
Magnetic solid-phase extraction was applied for sample pretreatment. The extraction efficiency for systemic pesticide residues was improved by optimizing sample preparation and pretreatment conditions. An automated and rapid detection method was established using a microarray biochip kit and a fully automatic biochip analyzer. The sensitivity and accuracy of the method were validated using cabbage, eggplant and tomato matrices.
The limits of detection and limits of quantitation for clothianidin, dimethomorph and carbendazim in cabbage, eggplant and tomato matrices met the detection requirements. The spiked recovery rates for the 3 kinds of vegetable matrices ranged from 81.48% to 120.48%, with relative standard deviations between 0.88% and 14.90%, demonstrating good accuracy and stability. The relative standard deviations in results between the proposed method and the confirmatory method for actual positive samples ranged from 0.12% to 9.95%, indicating good consistency.
This method exhibits high sensitivity and accuracy. The application of magnetic solid-phase extraction allows for rapid and simple removal of impurities from samples, effectively reducing instrument costs. The high-throughput and multi-parameter characteristics of the microarray biochip improve the coverage of detection items. Automation through instrumentation reduces the technical requirements for operators, providing technical support and a theoretical basis for the popularization and promotion of food safety testing.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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