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Detection of clothianidin and thiamethoxam in ginger by quantum beads based fluorescent immunochromatography
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Jian-Gang HU1, Yuan CHEN2, Kai LUO2, 3, Xing-Xing LOU1, Lei YAO1, Zhen-Zhen CHEN3, Wei-Hua LAI3, *
Journal of Food Safety & Quality | 2026, 17(3) : 300 - 308
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Journal of Food Safety & Quality | 2026, 17(3): 300-308
• Food Analysis and Detection •
Detection of clothianidin and thiamethoxam in ginger by quantum beads based fluorescent immunochromatography
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Jian-Gang HU1, Yuan CHEN2, Kai LUO2, 3, Xing-Xing LOU1, Lei YAO1, Zhen-Zhen CHEN3, Wei-Hua LAI3, *
Affiliations
  • 1.Shaoxing Testing Institute of Food and Drug, National Center for Quality Inspection and Testing of Chinese Rice Wine, Shaoxing 312000, China
  • 2.Jiangxi Weibang Biotechnology Co., Ltd., Nanchang 330029, China
  • 3.National Key Laboratory of Food Science and Resource Exploration, Nanchang University, Nanchang 330047, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251114001
Outline
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Objective

To establish a quantum dot microsphere based fluorescent immunochromatographic method for the rapid, sensitive and simultaneous detection of clothianidin and thiamethoxam in ginger.

Methods

In this study, the labeling pH, antibody labeling concentration and reaction buffer system of the 2 items were optimized.The fluorescence signal intensity of the test line and quality control line on the test strip was measured by the quantum dot microspheres test strip detector. The concentration of clothianidin and thiamethoxam was used as the abscissa, and the ratio of fluorescence signal intensity of the test line and quality control line was used as the ordinate to establish a standard curve.

Results

The quantitative detection ranges were 0.0625–8.0000 μg/L for clothianidin and 0.125–16.000 μg/L for thiamethoxam. The minimum limits of detection for thiamethoxam were 0.032 μg/L and for thiamethoxam were 0.070 μg/L.The recovery rates of standard addition for clothianidin ranged from 84.60% to 124.05%, and for thiamethoxam from 88.27% to 116.40%. The cross-reaction rate with 8 kinds of common drugs is less than 1%.

Conclusion

This method features unified sample pretreatment, simple and rapid operation, high sensitivity and simultaneous quantitative results for 2 analytes, making it suitable for on-site screening of large batches of samples.

quantum beads  /  clothianidin  /  thiamethoxam  /  detection  /  fluorescent immunochromatography
胡建刚, 陈媛, 罗凯, 娄行行, 姚蕾, 陈珍珍, 赖卫华. 量子点微球荧光免疫层析法检测生姜中噻虫胺和噻虫嗪. 食品安全质量检测学报, 2026 , 17 (3) : 300 -308 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251114001
Jian-Gang HU, Yuan CHEN, Kai LUO, Xing-Xing LOU, Lei YAO, Zhen-Zhen CHEN, Wei-Hua LAI. Detection of clothianidin and thiamethoxam in ginger by quantum beads based fluorescent immunochromatography[J]. Journal of Food Safety & Quality, 2026 , 17 (3) : 300 -308 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251114001
Appendix
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Year 2026 volume 17 Issue 3
8
1
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251114001
  • Receive Date：2025-11-14
  • Online Date：2026-11-05
Article Data
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History
  • Received：2025-11-14
Funding
Affiliations
    1.Shaoxing Testing Institute of Food and Drug, National Center for Quality Inspection and Testing of Chinese Rice Wine, Shaoxing 312000, China
    2.Jiangxi Weibang Biotechnology Co., Ltd., Nanchang 330029, China
    3.National Key Laboratory of Food Science and Resource Exploration, Nanchang University, Nanchang 330047, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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