Full
Outline
To develop a nanozyme-based immunochromatographic test strip with high sensitivity for rapid identification of bovine milk or goat milk adulteration in camel milk, utilizing the property that nanozymes can catalyze substrates to amplify detection signals.
The competitive method was adopted to develop the immunochromatography test strip. The rabbit antibody (Ab) of β-casein labeled with cobalt iron oxide (CoFe2O4)nanozyme was sprayed onto the binding pad. The β-casein antigen and mouse anti-rabbit immunoglobulin G (IgG) were respectively coated on the detection line (T line) and the control line (C line) of the nitrocellulose membrane to assemble the nanozyme immuno-chromatographic test strip. The nanozyme test strip was evaluated for consistency, sensitivity, limit of detection and specificity with the commercial colloidal gold immuno-chromatographic kit.
The nanozyme strip demonstrated a sensitivity of 5 μg/mL for antigen detection, which was 200 times higher than that of the commercial colloidal gold strip (1000 μg/mL). The limit of detection was 0.02% (capable of detecting cow milk diluted up to 5000 times), demonstrating a significant advantage over commercially colloidal gold strips with a limit of detection was 0.1%. It exhibited excellent specificity and achieved 100% consistency with the commercial colloidal gold strip.
This method is simple to operate, and can quickly screen out adulteration in camel milk on the spot.It has higher sensitivity than the commercial colloidal gold reagent strip.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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