Full
Outline
To investigate the extraction efficiency of Pteris vittata polysaccharides via an ultrasound-assisted cellulase method, and explore its hypoglycemic and hypolipidemic activities.
Response surface methodology was applied to optimize the technical parameters for the ultrasound-assisted cellulase extraction of Pteris vittata polysaccharides. The in vitro hypoglycemic activity was evaluated by determining the inhibition rates of α-amylase and α-glucosidase activities, while the in vitro hypolipidemic activity was assessed by analyzing the inhibition rate of pancreatic lipase activity as well as the binding rates to sodium glycocholate and sodium taurocholate.
The optimal extraction parameters for Pteris vittata polysaccharides were determined as follows: solid-liquid ratio of 1:20 (g:mL), cellulase dosage of 2.2%, ultrasonic power of 316 W, ultrasonic temperature of 51 ℃ and ultrasonic time of 22 min. Under these conditions, the extraction yield of Pteris vittata polysaccharides was measured to be 4.19%±0.08%. In vitro bioactivity assessment demonstrated that Pteris vittata polysaccharides possessed certain hypoglycemic and hypolipidemic biological activities. The half-maximal inhibitory concentrations (IC50) of Pteris vittata polysaccharides against α-amylase and α-glucosidase were 3.52 mg/mL and 2.75 mg/mL, respectively. Its IC50 against pancreatic lipase was 4.08 mg/mL, and the half-maximal effective concentrations (EC50) for glycocholate and taurocholate were 4.45 mg/mL and 4.38 mg/mL, respectively.
The ultrasound-assisted cellulase method can efficiently extract Pteris vittata polysaccharides. Pteris vittata polysaccharides are potential functional food material with hypoglycemic and hypolipidemic properties.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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