Full
Outline
To investigate the contamination status of Listeria monocytogenes in the production and processing of Flammulina velutipes in Lianyungang City.
Samples were collected from the production and processing chain of Flammulina velutipes in Lianyungang City. Isolation, identification and multilocus sequence typing of Listeria monocytogenes were performed.
A total of 67 samples were positive for Listeria monocytogenes among 359 samples collected from the production and processing chain of Flammulina velutipes, with an overall detection rate of 18.66%. The differences in the detection rate of Listeria monocytogenes among different sampling years and sampling locations were statistically significant (P<0.05), whereas no significant difference was observed among different sample types (P>0.05). The contamination rate in 2024 was relatively high(24.71%), and the positive detection rate of samples from the mycelium scraping room (38.46%) was much higher than that from other sampling sites. Environmental samples showed the highest contamination rate (25.32%).Sequence typing (ST) of the 67 Listeria monocytogenes isolates revealed that ST87 (32 isolates) and ST8 (31 isolates)were the dominant sequence types.
Listeria monocytogenes contamination exists in the production and processing chain of Flammulina velutipes in Lianyungang City, with the scraping room and harvesting and packaging area being the most heavily contaminated sites. It is essential to strengthen monitoring and supervision in these links, and formulate targeted prevention and control measures focusing on highly contaminated areas and environmental management.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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