Full
Outline
To establish a method for the determination of multiple mycotoxins in wheat and its products by direct extraction-ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).
Samples (2 g) were extracted with 10 mL 80% acetonitrile-water solution (containing 1% formic acid).After ultrasonic extraction, centrifugation, nitrogen blow-drying and reconstitution, the analytes were detected using an electrospray ionization (ESI) source in both positive and negative ion modes. Quantification was performed using the external standard method calibrated with a wheat flour matrix-matched standard curve.
The linear relationship of the 7 kinds of mycotoxins was good within a certain mass concentration range, with a determination coefficient (r2) greater than 0.998. The limits of quantitation were 0.40–7.70 μg/kg. The spiked recovery rates at the 3 levels were 77.9%–109.2%, and the relative standard deviations were 0.9%–9.0% (n=6).
The method has excellent precision and high sensitivity, it is suitable for the determination and high-throughput screening of mycotoxins in wheat and its products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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