Full
Outline
To determine the best medium composition and fermentation conditions to maximize the production of exopolysaccharides from Lentinula edodes through submerged fermentation, as well as to analyze the structure and assess the antioxidant properties of the extracted exopolysaccharides.
Single-factor experiments were conducted to identify the optimal carbon and nitrogen sources and their concentrations. Furthermore, 3 key factors influencing the yield of Lentinula edodes exopolysaccharides—pH, liquid volume and agitation speed were selected for response surface methodology optimization under controlled fermentation parameters (pH, liquid volume, rotation speed, inoculum size and fermentation time). The exopolysaccharides produced under these optimized conditions were characterized using scanning electron microscopy, ultraviolet spectroscopy, infrared spectroscopy and thermogravimetric analysis. Their ability to scavenge free radicals, including 1, 1-diphenyl-2-trinitrophenylhydrazine(DPPH), 2, 2-biazo-di (3-ethyl-benzothiazolin-6-sulfonic acid) diammonium salt, and hydroxyl radicals, was also evaluated.
Glucose at 20 g/L and yeast extract at 5 g/L were identified as the optimal carbon and nitrogen sources, respectively, for submerged fermentation of Lentinula edodes. The ideal fermentation parameters were pH 6, liquid volume of 101 mL, agitation speed of 149 r/min, inoculum size of 5% and an 8-day fermentation period. Scanning electron microscope images showed that the exopolysaccharides had a loose structural morphology. Ultraviolet spectroscopy revealed no absorption peaks indicative of nucleic acids or proteins, while infrared spectroscopy displayed characteristic polysaccharide absorption bands at 3408.1, 2920.5, 1646.1 and 1324.5 cm–1. Thermogravimetric analysis demonstrated that the polysaccharides maintained good thermal stability between 28 ℃ and 180 ℃. The exopolysaccharides exhibited strong antioxidant activity, with scavenging rates of 60.14% for DPPH radicals, 53.47% for ABTS cationic radicals and 93.94% for hydroxyl radicals.
The study optimize the submerged fermentation process for producing exopolysaccharides from Lentinula edodes, confirming the method’s stability and practicality. These findings provide a theoretical foundation for enhancing fermentation efficiency, increasing polysaccharide yields and supporting further development and application of Lentinula edodes polysaccharides.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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