Full
Outline
To optimize the process conditions for the preparation of Juglans regia L. antioxidant peptides by echelon enzymatic hydrolysis.
Juglans regia L. antioxidant peptides were prepared from defatted Juglans regia L. powder by alkaline protease, neutral protease and acid protease in a consecutive manner. The enzymatic hydrolysis process was optimized by single factor combined with orthogonal experiment and response surface method with antioxidant activity and hydrolysis degree as indexes.
The optimum process of neutral protease was enzymatic hydrolysis temperature 51 ℃, pH 6.87, substrate concentration 5%, enzyme addition 28000 U, enzymatic hydrolysis time 3 h. The optimum process of acid protease was enzymatic hydrolysis temperature 50.0 ℃, pH 5, substrate concentration 5.0%, enzyme addition 30000 U, enzymatic hydrolysis time 3 h. The degree of hydrolysis was 43.74%, the cation radical scavenging rate of 2, 2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) was 92.14%, and the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging rate was 87.55%.
This study optimizes the protein echelon enzymatic hydrolysis process, which can be used as a feasible strategy for the preparation of Juglans regia L. antioxidant peptides.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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