Full
Outline
To develop an electrochemiluminescence (ECL) test strip for the detection of aflatoxin B1(AFB1).
The ECL test strip was fabricated by patterning carbon ink electrode onto the surface of a polyvinyl chloride substrate and then assembling them with a lateral flow immunoassay strip. The electrochemical performance of the ECL test strip was investigated using cyclic voltammetry. Meanwhile, Ru-PEI/Ti3C2Tx@Au composites were synthesized and conjugated with AFB1 monoclonal antibodies (Anti-AFB1) to serve as ECL signal probes. Through the competitive immunoreaction between the AFB1-bovine serum albumin conjugate immobilized on the test line of the ECL test strip and the ECL signal probes, colorimetric and ECL signals were obtained.
The fabricated ECL test strips exhibited good stability and specificity. Under optimal conditions, the colorimetric signal showed a good linear relationship with the logarithm of AFB1 concentration in the range from 0.1 ng/mL to 100.0 ng/mL, with a limit of detection of 32 pg/mL. In addition, the ECL signal showed a good linear relationship with the logarithm of AFB1 concentration in the range from 1.0 pg/mL to 100.0 ng/mL, with a limit of detection of 319 fg/mL. Furthermore, satisfactory recovery rates ranging from 92.6% to 110.3% were achieved by the proposed ECL test strips, along with relative standard deviations of 0.9% to 6.2%.
The developed ECL test strip enables highly sensitive and rapid detection of AFB1 in corn samples, providing a new perspective for rapid mycotoxin detection.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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