Full
Outline
To determine the content of 11 kinds of organic acids (citric acid, malic acid, tartaric acid, succinic acid, maleic acid, lactic acid, acetic acid, tannic acid, fumaric acid, gallic acid and coumaric acid) in green plum wine by high performance liquid chromatography (HPLC) and the chemical pattern recognition analysis is performed.
The analysis was performed using HPLC with a CAPECELL PAK ADME C18 (4.6 mm×250 mm, 5 μm)column, where mobile phase A was methanol and mobile phase B was a 0.1% (V:V) aqueous phosphoric acid solution.Gradient elution was employed at a column temperature of 30 ℃, with a detection wavelength of 210 nm. The results were subsequently analyzed using chemopattern recognition software, specifically SPSS 25.0 and SIMCA 14.1.
The 11 kinds of organic acids exhibited excellent linearity (r≥0.9993) within their respective mass concentration ranges, with average addition recovery ranging from 92.69% to 104.35% and relative standard deviations of 0.29% to 4.72%. Cluster analysis of 9 batchs samples revealed 3 distinct groups when Euclidean distance was set to 5. Principal component analysis identified 3 major components, consistent with cluster results: the first principal component included tartaric acid, malic acid, tannic acid, citric acid, succinic acid and fumaric acid; the second principal component comprised acetic acid and gallic acid; while the third principal component consisted of lactic acid, maleic acid and coumaric acid. Orthogonal partial least squares discriminant analysis identified citric acid and malic acid as potential flavor difference markers.
The method is simple, accurate and sensitive, and is suitable for qualitative and quantitative analysis and evaluation of organic acids in green plum wine and other foods.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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