Full
Outline
To screen lactic acid bacteria from sauerkraut fermented with Wudalianchi natural mineral spring water, and to investigate their probiotic properties.
MRS-CaCO₃ agar medium was used for the primary screening of lactic acid bacteria from the samples. The growth curves of the isolated strains were plotted, and physiological, biochemical and molecular biological identifications were performed. Moreover, the probiotic properties including acid production, acid tolerance, bile salt tolerance and bacteriostatic activity were analyzed.
A total of 6 lactic acid bacteria strains were isolated and identified as Leuconostoc mesenteroides, Lactococcus lactis, Lactococcus cremoris, Lactiplantibacillus pentosus, Weissella hellenica and Weissella confusa, respectively. Growth curve analysis showed that 3 of the strains entered the logarithmic growth phase within 4 h and reached the stationary phase at 18–21 h. The evaluation results of probiotic properties indicated that strain 14-1, KQ15-1 (Lactococcus lactis)had the fastest acid production rate, while strain KQ19-1 (Lactiplantibacillus pentosus) exhibited the strongest acid production capacity with the final pH of 3.3. Strain KQ19-1 also showed the highest acid tolerance, with survival rates of 99%, 90% and 81% at pH 3.5, 3.0 and 2.5, respectively. In addition, strain KQ19-1 presented the highest bile salt tolerance with a survival rate of 97% under 0.3% bile salt concentration, whereas strain KQ10-1 (Leuconostoc mesenteroides) had the weakest bile salt tolerance with a survival rate of only 7%. All the 6 strains were sensitive to penicillins, cephalosporins and macrolides without drug resistance. Notably, strain KQ19-1 exerted bacteriostatic activity against 3 kinds of pathogenic bacteria.
Strain KQ19‑1 screened in this study has the best probiotic characteristics. It can provide basic data for the exploration of probiotic resources from sauerkraut fermented with natural mineral spring water and the quality improvement of sauerkraut fermented with traditional method.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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