Full
Outline
To extract and prepare highly active hypoglycemic polysaccharides from Auricularia heimuer and evaluate their activity.
The enzymatic hydrolysis process was optimized through single-factor and orthogonal experiments to obtain the optimal α-glucosidase inhibitory activity. After deproteinization of the crude polysaccharides used the trichloromethane-n-butanol method, the polysaccharides were purified successively through a diethylaminoethyl cellulose 52 anion exchange packing column and a high-resolution sephacryl S-200 gel column. The refined Auricularia heimuer polysaccharide component (AAPS4s) was obtained.
Under the optimal conditions, the inhibition rate of crude polysaccharides on α-glucosidase was 76.38%±0.94%. The content of AAPS4s polysaccharide was 95.63%±1.46%, and the inhibitory rates against α-glucosidase and α-amylase were 72.56%±2.12% and 41.90%±0.87, respectively. The inhibitory type was mixed; the scavenging rates of 1, 1-diphenyl-2-picrylhydrazyl free radicals, hydroxyl radicals and superoxide anions were 57.39%±0.73%, 64.99%±2.65% and 38.41%±2.80%, respectively.
This study successfully obtains the highly active Auricularia heimuer polysaccharide component AAPS4s with clear activity. This component regulates digestive enzyme activity in a mixed inhibitory mode to exert in vitro hypoglycemic effects and possesses good antioxidant activity, laying a foundation for the development and industrial application of Auricularia heimuer hypoglycemic functional products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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