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Outline
To evaluate the genotoxicity and in vitro hypolipidemic activity of docosahexaenoic acid-eicosapentaenoic acid-rich oil from Schizochytrium (DHA-EPA SO) (DHA content: 37.33%±0.15%; EPA content: 5.82%±0.03%), clarify its safety and functional properties.
Using the bacterial reverse mutation assay, mammalian erythrocyte micronucleus test and mouse spermatocyte chromosome aberration test, the genotoxicity of DHA-EPA SO was systematically evaluated. Using 3T3-L1 preadipocytes as the cell model, the in vitro hypolipidemic activity of DHA-EPA SO was investigated via qualitative observation by oil red O staining, quantitative analysis of optical density value, and determination of intracellular triacylglycerol (TG) and total cholesterol (TC) content.
Within the test dose range, DHA-EPA SO presented negative results in 3 genotoxicity assays. In the bacterial reverse mutation assay, the number of revertant colonies of each strain did not exceed twice that of the negative control group; the micronucleus rate of mouse bone marrow erythrocytes and the chromosome aberration rate of mouse spermatocytes showed no significant differences compared with the control groups (P>0.05), indicating no mutagenicity or genetic damage risk of DHA-EPA SO. The in vitro hypolipidemic tests demonstrated that DHA-EPA SO could effectively inhibit lipid accumulation in 3T3-L1 cells and reduce intracellular TG and TC content, exerting the optimal hypolipidemic effect at a mass concentration of 12.50 μg/mL, while the hypolipidemic activity was attenuated with increased doses.
DHA-EPA SO has no genotoxicity and exhibits significant in vitro hypolipidemic activity, which endows it with broad application prospects in the field of functional foods and health products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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