Full
Outline
To establish a method for simultaneous determination of 6 kinds of synthetic sweeteners in tea and tea products by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry.
Samples were extracted with acetonitrile-water (8:2, V:V), salted out with anhydrous magnesium sulfate and sodium acetate, and purified via dispersive solid-phase extraction using octadecylsilane adsorbent and N-propylethylenediamine adsorbent sorbents. Chromatographic separation was performed on a Waters ACQUITY UPLC HSS T3 column (100 mm×2.1 mm, 1.8 μm) using gradient elution with a water-acetonitrile mobile phase at a flow rate of 0.2 mL/min and an injection volume of 5 μL. The analytes were detected in electrospray negative-ion multiple reaction monitoring mode and quantified by external standard method.
The linear relationship of the 6 kinds of synthetic sweeteners was good within the mass concentration range of 2–1000 μg/kg (r>0.99). The limits of detection were 5–25 μg/kg and the limits of quantitation were 15–75 μg/kg. The average recoveries at 100, 500, 2500 μg/kg spiked levels ranged from 88.34%–103.21%, with relative standard deviations of 0.56%–7.63%.
The proposed method is simple,rapid, sensitive, accurate and reliable, making it well suited for the simultaneous rapid detection of various synthetic sweeteners in tea and tea products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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