Full
Outline
To comparatively study the Bact/Alert 3D microbiological detection system method(referred to as the instrument method) in the detection of commercial sterility items of ultra-high temperature instantaneous (UHT) sterilized milk, pure milk and room-temperature yogurt, with reference to the national standard methods.
The inclusivity of the instrument method was assessed by performing suitability tests on 36 kinds of microbial species. The sensitivity and detection time of the 2 methods were compared by detecting UHT pure milk artificially contaminated with Bacillus licheniformis and room-temperature yogurt artificially contaminated with Candida albicans using the GB 4789.26—2023 National food safety standard-Food microbiological examination-Commercial sterility testing (national standard method) and the instrument method; sensitivity was compared using 50% limit of detection (LOD50), and the detection timeliness was evaluated. Additionally, the sensitivity was validated across 5 laboratories.
The instrument method demonstrated 100% inclusivity for the 36 kinds of microbial species tested. The LOD50 of the instrument method was slightly lower than that of the national standard method for UHT pure milk with Bacillus licheniformis, but slightly higher for room-temperature yogurt with Candida albicans. The time to positivity for the instrument method was significantly shorter than that of the national standard method.
The instrument method shows good applicability for the 36 kinds of microbial species tested.While there are slight differences in sensitivity compared to the national standard method depending on the sample matrix, the instrument method offers superior timeliness.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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