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Outline
To establish an improved sample pretreatment procedure combined with gas chromatography (GC) coupled with an electron capture detector (ECD) and gas chromatography-tandem mass spectrometry (GC-MS) for the determination of prochloraz and its metabolites in fruits and vegetables.
Prochloraz and its metabolites were extracted from samples using a solvent mixture based on anhydrous ethanol and dichloromethane. The extract was subjected to rotary evaporation, followed by hydrolysis with anhydrous magnesium sulfate in the presence of pyridine hydrochloride, re-extraction with dichloromethane, purification with sulfuric acid, and concentration. The final residue was reconstituted with a mixture of methyl tert-butyl ether and ascorbic acid-saturated acetone solution (9:1, V:V), filtered through a syringe filter and analyzed.
The spiked recoveries of prochloraz and its metabolites in fruits and vegetables were determined using the proposed method. The GC-ECD method exhibited recoveries ranging from 95% to 118%, with relative standard deviations of 3.95%–4.89% (n=6) and limit of detection of 0.01 mg/kg. The GC-MS method achieved recoveries of 92%–108%, with relative standard deviations of 3.39%–4.35%(n=6) and an limit of detection of 0.001 mg/kg.
Compared with the current national food safety standard GB 23200.121—2021 National standard for food safety food-Determination of residues of 352 pesticides and their metabolites in plant-derived foods-Liquid chromatography-tandem mass spectrometry, this method enables the analysis of prochloraz and its metabolite residues in fruits and vegetables within a shorter time frame while offering advantages in terms of sensitivity, accuracy and cost. It provides a reliable technical approach and research foundation for the detection of prochloraz and its metabolites in complex fruit and vegetable matrices.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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