Full
Outline
To assess the applicability of the MicroFast® Staphylococcus aureus test strips method for microbiological testing of food products.
This study presented a comprehensive validation of the test chart method, followed the performance specifications set out in the International Organization for Standardization ISO 16140—2: 2016. The validation covered its inclusiveness, exclusiveness and consistency with the test results of the current national standards. The results of both methods were statistically analyzed using t-test and Bland-Altman.
All 30 Staphylococcus aureus target strains showed typical purple colony characteristics on MicroFast test slices; 3 of the 30 non-target strains had atypical blue colonies, and no colony growth was observed colony growth on 27 strains the test slices. After correlation analysis, the MicroFast® Staphylococcus aureus test piece method showed a significant positive correlation with the results of the second method of GB 4789.10—2016 Food safety national standard-Food microbiological test-Staphylococcus aureus test, with a correlation coefficient (r) value of more than 0.976. The suitability of the test slices was also evaluated for a variety of food types, and the calculated β-expected tolerance intervals were all within ±0.5 logarithmic range.
MicroFast® Staphylococcus aureus test pieces have good inclusiveness and exclusivity, consistency and equivalence with current national standard methods, and are suitable for the quantitative detection of Staphylococcus aureus in food.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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