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Outline
Foodborne pathogenic bacteria are one of the major factors leading to food safety incidents.Traditional detection methods have limitations such as time consumption, low sensitivity and complicated operation.In recent years, with the rapid development of molecular biology, immunology, biosensor technology and point-of-care testing (POCT) technology, a variety of rapid detection technologies for foodborne pathogenic bacteria have emerged. This paper systematically reviewed the latest research progress in the field of rapid detection of pathogenic bacteria in food, focused on the principles and characteristiced of molecular biology technologies (mass spectrometry, polymerase chain reaction and its derivative technologies, isothermal amplification, high-throughput sequencing) and immunological technologies (enzyme-linked immunosorbent assay, immunochromatography,immunosensors). It also summarized the application status of POCT technology formed by the interdisciplinary integration of molecular biology, nanotechnology, immunology and information technology. Meanwhile, it analyzed the challenges faced by various technologies, and prospected the future development trends, aimed to provide technical references for the monitoring of pathogenic bacteria in food.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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