Full
Outline
To establish a method to determine the quality of safflower yellow in different manufacturers by high performance liquid chromatography (HPLC) fingerprint chromatogram method, and perfect the quality evaluation system of safflower yellow extract.
HPLC analysis was carried out on a C18 chromatographic column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.05% trifluoroacetic acid aqueous solution with gradient elution. The flow rate was set at 1.0 mL/min, column temperature at 30 ℃,detection wavelength at 266 nm, and injection volume at 10 μL. The 16 batches of safflower yellow extract were systematically classified and analyzed via similarity evaluation, cluster analysis and principal component analysis;meanwhile, the content of hydroxysafflor yellow A was determined.
The 11 common characteristic peaks were identified in safflower yellow extract, and 6 of these peaks were assigned. Cluster analysis and principal component analysis demonstrated distinct classification differences among samples from different manufacturers.Orthogonal partial least squares-discriminant analysis (OPLS-DA) screened out 5 major marker components with variable importance in the projection (VIP) values>1; these components exhibited significant fluctuations in products from different manufacturers and could serve as key quality indicators. In terms of hydroxysafflor yellow A content,the quality of commercially available safflower yellow extract was uneven, which might be attributed to the planting environment of raw materials, harvesting and processing techniques, as well as storage conditions.
This study established a comprehensive quality evaluation system combining HPLC fingerprint analysis, chemometrics,and marker component quantification, which provides a scientific basis for refining the quality evaluation system and revising the quality standards of safflower yellow extract.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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