Full
Outline
To investigate the blood glucose regulatory effects of Pediococcus acidilactici AUI2505 and Limosilactobacillus reuteri AUI2506 on hyperglycemic Danio rerio.
The in vitro probiotic properties and safety of 2 strains of lactic acid bacteria were evaluated through simulated gastrointestinal fluid tolerance tests,antibiotic susceptibility assays, hemolytic activity assessment and analyses of hydrophobicity and auto-aggregation ability. A hyperglycemic Danio rerio model was established by immersing the fish in water containing 3% glucose to induce blood glucose metabolism disorder, after which the Danio rerio were treated with the bacterial strains. After intervention, the survival rate, blood glucose levels, insulin levels, inflammatory factors and glucose tolerance of the Danio rerio in each group were measured.
Strains AUI2505 and AUI2506 exhibited survival rates greater than 80% in simulated gastric fluid and greater than 40% in simulated intestinal fluid, respectively. Both strains were sensitive to most antibiotics tested and non-hemolytic. The 2 strains exhibited favorable hydrophobicity and auto-aggregation capacity. In the hyperglycemic Danio rerio model, it was demonstrated that following intervention with bacterial suspensions of AUI2505 and AUI2506, all experimental groups exhibited normal larval survival rates,blood glucose and inflammatory cytokine levels were significantly decreased (P<0.05), and glucose tolerance was significantly enhanced (P<0.05). Additionally, a significant reduction in insulin level was shown in the AUI2505 group (P<0.05).
Pediococcus acidilactici AUI2505 and Limosilactobacillus reuteri AUI2506 have significant hypoglycemic effects, providing a theoretical foundation for their use in adjunctive probiotic treatment for type 2 diabetes mellitus.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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