Full
Outline
To construct a fluorescent biosensor composed of 2’-fluoro-substituted nucleic acid aptamer (2’F-Apt) and graphene oxide (GO) for the highly sensitive and specific detection of Staphylococcus aureus in prepackaged foods.
The biosensor was fabricated via non-covalent adsorption and assembly between 2’F-Apt and GO. The fluorescence of the aptamer was quenched by GO. In the presence of target bacteria, 2’F-Apt specifically bound to surface proteins of the bacteria and detached from GO, resulting in the recovery of fluorescence signal, which enabled the quantitative detection of samples.
Compared with unmodified nucleic acids,2’-fluoro substitution reduced the conformational constraints of nucleic acid strands and improved molecular flexibility. It also increased binding sites and specific surface area, strengthened the interaction between 2’F-Apt and GO, effectively inhibited non-specific dissociation and reduced false-positive results. Meanwhile, the target recognition capability and detection sensitivity were enhanced. The test results showed that the linear detection range of the 2’F-Apt/GO biosensor for Staphylococcus aureus was 10 to 109 CFU/mL, with a limit of detection of 5 CFU/mL, presenting excellent detection performance.
The biosensor constructes in this study features simple preparation, good universality and high sensitivity, and can be applied to the rapid detection of Staphylococcus aureus in prepackaged foods.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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