To investigate the amino acid composition characteristics and nutritional value of Cyperus esculentus from 11 provinces and autonomous regions in China (Xinjiang, Hebei, Henan, Shandong, Hunan, Ningxia,Yunnan, Inner Mongolia, Heilongjiang, Liaoning and Jilin), and establish a geolocation model based on amino acid composition.
Amino acid analyzers were used to test Cyperus esculentus samples from different varieties across these 11 provinces and autonomous regions, analyzing the effects of origin and varying climatic conditions on amino acid content. From a nutritional perspective, the nutritional value of Cyperus esculentus amino acids across the 11 provinces and autonomous regions was comprehensively evaluated through analysis of essential amino acids,branched-chain amino acid composition and amino acid scoring results.
Cyperus esculentus exhibited a rich variety of amino acids, with essential amino acids accounting for 24%–36% and branched-chain amino acids ranging from 12.02%–17.43%. Amino acid scoring indicated that methionine was the primary limiting amino acid in Cyperus esculentus across all 11 provinces and autonomous regions. In actual consumption, it was recommended to address methionine deficiency through dietary combinations or nutritional fortification. Under the subtropical monsoon climate of Southwest Asia, the levels of glutamic acid and aspartic were significantly higher than under other climatic conditions. Using chemometric software, 3 kinds of models—K-nearest neighbor algorithm (KNN), partial least squares discriminant analysis (PLS-DA) and support vector machine (SVM)—were applied for origin tracing. The KNN model demonstrated the best performance, achieving a training set recognition rate of 96.97% and a cross-validation recognition rate of 98.99%.
This study investigates amino acid content variations in Cyperus esculentus across different provinces and autonomous regions, providing a reference for Cyperus esculentus cultivation and subsequent industrialization of Cyperus esculentus products.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |