Full
Outline
To establish a quantitative analysis method for determining illegally added uric acid-lowering substances in food by ultra performance liquid chromatography-tandem mass spectrometry.
The sample was extracted with methanol and 5% methanol solution as the extraction solvent, and extracted by ultrasound, and then separated on a C18 column with a gradient elution of 0.1% form acid aqueous solution and methanol as the mobile phase. The detection was performed using multiple reaction monitoring (MRM) mode of mass spectrometry, and quantification was done by external standard method.
In the solvent curve such as allopurinol, colchicine, topiroxostat, lesinurad and tranilast all showed linear correlation coefficients of r>0.999 in the range of 20–400 ng/mL, while risperidone, fenofibrate, febuxostat, probenecid and benzbromarone showed linear correlation coefficients of r>0.999 in the range of 2–40 ng/mL. The recovery rates ranged from 81.62% to 109.61% within 3 levels in 5 kinds of matrices such as tablets (compressed candy tablets), liquid beverage, powdered beverage,pollen and effervescent tablet and the relative standard deviations (RSDs) were 1.0% to 8.6% (n=6).
This method has high accuracy, good precision, high reproducibility and good applicability between instruments, and can meet the needs of qualitative and analysis of allopurinol and other 10 kinds of uric acid-lowering compounds in food.
6
1
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
