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Outline
To establish a method for the determination of illegal additive caramel in Mume fructus by high performance liquid chromatography.
After the sample was extracted with water, ethanol was added for precipitation, the supernatant was discarded, and the sample was reconstituted with water and filtered. Luna SCX 100 Å (250 mm×4.6 mm, 5 μm) chromatography column was selected. The 10 mmol/L sodium dihydrogen phosphate was used as the mobile phase for isocratic elution and detected by a diode array detector.
Caramel exhibited a good linear relationship with peak area within the range of 0.0243–2.4300 mg/mL (r=0.9999);the limit of detection was 0.10 mg/kg; the limit of quantitation was 0.19 mg/kg; the average recovery rate was 90.1%,and the relative standard deviation was 1.8% (n=6).
This method has good sensitivity and high recovery rate, and is suitable for the detection of illegal additive caramel in Mume fructus.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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