Full
Outline
To establish a method for the simultaneous determination of 9 kinds of food additives in foods by ultra performance liquid chromatography.
The samples were subjected to ultrasonic extraction with water, purified by a PWA-2 solid-phase extraction cartridge, and then separated on a Poroshell 120 EC-C18 column (150 mm×3.0 mm, 2.7 μm) under gradient elution using a mobile phase composed of 25 mmol/L potassium dihydrogen phosphate solution and acetonitrile. Detection was carried out at 215 nm.
The results showed good linearity for the 9 kinds of analytes in the mass concentration range of 2.0–100.0 mg/L, with correlation coefficients (r2) not less than 0.999. The limits of detection and limits of quantitation ranged from 0.0025 to 0.0050 g/kg and from 0.005 to 0.010 g/kg, respectively. Recovery experiments were performed at low, medium and high spiked levels in 4 kinds of representative matrices (tea beverage, sausage, milk, red wine). The average recoveries were 80%–118%, with relative standard deviations in the range of 2.8%–8.5% (n=6).
The method exhibited high sensitivity, good reproducibility and high accuracy, and is applicable for the simultaneous determination of 9 kinds of food additives,including tannic acid, in food matrices.
6
1
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
