Full
Outline
To establish a method for the determination of total arabinoxylan content in cereals by microwave digestion-high performance liquid chromatography-evaporative light scattering detection.
Following digestion with 1.0 mol/L trifluoroacetic acid at 120 ℃ for 30 min, the samples were de-acidified,re-dissolved and subsequently analyzed. Separation was performed on an NH2 chromatographic column (4.6 mm×250 mm, 5 µm) with isocratic elution using acetonitrile-water (90:10, V:V) as the mobile phase. Detection was conducted using an evaporative light scattering detection, quantification was performed by the external standard method, and the developed method was applied to the analysis of common cereal samples.
After methodological verification, good linearity was observed for xylose and arabinose in the range of 0.2 to 2.0 mg/mL,with coefficient of determination exceeding 0.999. The limits of detection were 1.0 mg/g for xylose and 1.5 mg/g for arabinose, and the corresponding limits of quantitation were 2.5 mg/g and 5.0 mg/g. Recoveries ranged from 86.8% to 93.1% (relative standard deviation all less than 3.5%).
The method is accurate, reliable and sensitive,making it suitable for the determination of total arabinoxylan in cereals and providing a reference for cereal quality evaluation, processing adaptability analysis and dietary nutrition research.
7
1
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
