To establish a method for simultaneous detection of 38 kinds of veterinary drug residues(including tetracyclines, sulfonamides, quinolones, β-agonists, amantadine, chloramphenicol, thiamphenicol, etc.) in raw pork puree for infant complementary foods by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).

Samples were extracted with acetonitrile and ethylenediaminetetraacetic acid disodium salt (Na₂EDTA)–Mcllvaine buffer, cleaned-up on an Oasis PRiME HLB cartridge, concentrated to dryness under nitrogen at 40 °C and reconstituted in 0.2% formic acid. Chromatographic separation was performed on a Phenomenex Kinetex® 2.6 µm EVO C₁₈ column, and the analysis was conducted using multiple reaction monitoring with dynamic polarity switching via an Agilent Jet Stream electrospray ionization source (AJS ESI), with quantification based on a matrix-matched calibration standard approach.

The 10 kinds of target compounds and 28 kinds of target compounds showed good linear responses in the concentration ranges of 10–400 µg/L and 10–500 µg/L, respectively, and the correlation coefficients (r) of the obtained calibration curves were all no less than 0.995. The limits of detection were determined to range from 0.03 to 1.47 µg/kg, with the limits of quantitation established between 0.10 and 4.90 µg/kg. At 3 spiked levels of 5, 10 and 20 µg/kg (n=6), the average recoveries ranged from 64.32% to 111.66% with relative standard deviations of 2.17%–10.70%, meeting the requirements specified in GB/T 27404—2008 Specifications for quality control in laboratories-Physical and chemical testing of food.

This method offers straightforward operation, high sensitivity, good accuracy and precision,enabling high-throughput screening and confirmation of 38 kinds of veterinary drug residues in pork puree used as raw material for infant complementary foods.