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Rapid identification of duck-derived components in mutton products by duplex real-time fluorescent polymerase chain reaction
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Shi-Chun YI1, Zi-Ying YAN1, Gang WANG2, Fu-Guo XING2, Chen-Chuan ZHANG3, Hong-Fei LI3, Er-Feng LI1, *
Journal of Food Safety & Quality | 2026, 17(10) : 110 - 118
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Journal of Food Safety & Quality | 2026, 17(10): 110-118
• Special Topic: Food Authenticity and Traceability •
Rapid identification of duck-derived components in mutton products by duplex real-time fluorescent polymerase chain reaction
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Shi-Chun YI1, Zi-Ying YAN1, Gang WANG2, Fu-Guo XING2, Chen-Chuan ZHANG3, Hong-Fei LI3, Er-Feng LI1, *
Affiliations
  • 1.College of Horticulture and Landscape Architecture, Tianjin Agricultural University, Tianjin 300392, China
  • 2.Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • 3.Zhangjiajie Bureau of Agriculture and Rural Affairs, Zhangjiajie 427000, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251217001
Outline
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Objective

To establish a method for the rapid identification of duck-derived components in mutton by duplex real-time fluorescence polymerase chain reaction (dRT-PCR).

Methods

First, the response surface methodology was used to optimize various conditions for the rapid extraction of DNA from animal tissues.Subsequently, multiple sets of specific primers were designed based on the differences between the sheep mitochondrial cytochrome b gene (CYTB) and the duck cytochrome c oxidase subunit III (COX3). The dRT-PCR experiment was conducted using the rapidly extracted animal tissue DNA. By analyzing the melting curve, the sheep and duck-derived components were qualitatively identified according to the differences in peak temperature.

Results

The optimized method for crude extraction of animal tissue DNA could extract 586 ng/μL of DNA suitable for species detection from 20 mg of animal tissue within 5 minutes. The sheep-derived gene products and duck-derived gene products produce melting peaks at 84.79 ℃ and 81.66 ℃ respectively, with no specific amplification for non-target species. It could quickly detect more than 0.01 ng/μL of crude duck DNA within 1 hour.

Conclusion

This method is simple to operate, low in cost, and has high sensitivity, providing effective technical support for the identification of adulteration in mutton products.

mutton products  /  duck-derived components  /  crude DNA extraction  /  response surface methodology  /  duplex real-time fluorescent polymerase chain reaction
易仕春, 燕姿颖, 王刚, 邢福国, 张陈川, 李红飞, 李二峰. 双重实时荧光聚合酶链反应法快速鉴定羊肉制品中鸭源性成分. 食品安全质量检测学报, 2026 , 17 (10) : 110 -118 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251217001
Shi-Chun YI, Zi-Ying YAN, Gang WANG, Fu-Guo XING, Chen-Chuan ZHANG, Hong-Fei LI, Er-Feng LI. Rapid identification of duck-derived components in mutton products by duplex real-time fluorescent polymerase chain reaction[J]. Journal of Food Safety & Quality, 2026 , 17 (10) : 110 -118 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251217001
Appendix
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Year 2026 volume 17 Issue 10
9
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251217001
  • Receive Date：2025-12-17
  • Online Date：2026-11-05
Article Data
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History
  • Received：2025-12-17
Funding
Affiliations
    1.College of Horticulture and Landscape Architecture, Tianjin Agricultural University, Tianjin 300392, China
    2.Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
    3.Zhangjiajie Bureau of Agriculture and Rural Affairs, Zhangjiajie 427000, China
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https://castjournals.cast.org.cn/joweb/spaq/EN/10.19812/j.cnki.jfsq11-5956/ts.20251217001
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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